laywhoish12
Well-Known Member
Damn , i want some shroomskiss-ass
Bwpz' First PF Tek Attempt
- Thread starter Bwpz
- Start date
Bwpz
Well-Known Member
Bwpz
Well-Known Member
Spore Syringe: $15
Half-pint jars: $7
Vermiculite: $8
Aluminum Foil: $1
Brow Rice Flour: $3
Perlite (24qts): $16
Terrarium: $15
Alcohol Lamp: $2
Analog Thermometer/Hygrometer: $10
Total: $77 ($41 not needed for 1 month after start of process) So $36 for first month to do it, not bad
Half-pint jars: $7
Vermiculite: $8
Aluminum Foil: $1
Brow Rice Flour: $3
Perlite (24qts): $16
Terrarium: $15
Alcohol Lamp: $2
Analog Thermometer/Hygrometer: $10
Total: $77 ($41 not needed for 1 month after start of process) So $36 for first month to do it, not bad
tryingtogrow89
Well-Known Member
total invest was 2 syringes for 18 and like 40 the rest of supplies which once started you can grow a lifetime of shrooms without ever ordering more spores again!Then grow someIt costs like $40...
Bwpz
Well-Known Member
total invest was 2 syringes for 18 and like 40 the rest of supplies which once started you can grow a lifetime of shrooms without ever ordering more spores again!
Yeah that's the good thing, free spores from there on unless you'd want a different strain
Do you have a good tutorial on making the syringes?
uromastyx
Well-Known Member
making syringes from prints can be a little tricky. Not the actual process but creating a steril environment. I can type some stuff up tomorrow morning when i have more time if no one else beats me to it, but cloning them is the way to go in my opinion no fluctuations in potency.
uromastyx
Well-Known Member
This is a good outline
http://www.shroomery.org/8405/How-to-make-a-spore-syringe
http://www.shroomery.org/8405/How-to-make-a-spore-syringe
Bwpz
Well-Known Member
Thanks man, I appreciate your input. Gives me another reason to invest in a glovebox xD
tryingtogrow89
Well-Known Member
MAKING A SPORE SYRINGE
Use a plastic (disposable) syringe with an 18 gauge 1 1/2 inch needle. You can blunt the needle to make it safe by simply sniping the tip of the needle with a wire cutter. You can resterilze the syringe and needle (wraped in tin foil) by boiling it for an hour.
Prepare a small bottle of sterilized water. Place the bottle and cap into the pot with a tight lid and boil for one hour. Then with a forceps or tong, get the cap loosely back on the bottle (while under the surface) and remove it. After cooling, tighten the bottle cap.
When ready, pour a little of this sterile water into the spore print jar and with a sterile knife blade, scrape the spores into the water. The water will become thick with purple specks. Swirl the water and load the syringes. Do the operation quickly and smoothly. Expose the jar opening as little as possible. Don't breathe on it. This syringe is good for several months. The spores fare well in their watery environment.
You can improve the sterile process by using the long needle technique. With a needle hole in the lid, water and spore solution can be loaded and unloaded from the jar with little air contact. Spores can be scraped into solution through the hole in the lid with a sterilized long blunted needle or implement, improving isolation and sterility. Extra long syringe needles (heavy gauge 3 1/2 inch - science supply) have their use here. The needles have to be blunted to work correctly at scraping spores off the bottom of a jar. Blunt them with a large wire cutter and finish the tip with a file. These type of needles can be bought, but finding them is the task. If questioned by the seller on what the use will be, just tell them the truth. Mycology is OK . Try your local veterinarian or science catalog retailer. The needle techniques are very versatile and can be employed in many ways good for spore solution preparation without the usual lab setups.
Use a plastic (disposable) syringe with an 18 gauge 1 1/2 inch needle. You can blunt the needle to make it safe by simply sniping the tip of the needle with a wire cutter. You can resterilze the syringe and needle (wraped in tin foil) by boiling it for an hour.
Prepare a small bottle of sterilized water. Place the bottle and cap into the pot with a tight lid and boil for one hour. Then with a forceps or tong, get the cap loosely back on the bottle (while under the surface) and remove it. After cooling, tighten the bottle cap.
When ready, pour a little of this sterile water into the spore print jar and with a sterile knife blade, scrape the spores into the water. The water will become thick with purple specks. Swirl the water and load the syringes. Do the operation quickly and smoothly. Expose the jar opening as little as possible. Don't breathe on it. This syringe is good for several months. The spores fare well in their watery environment.
You can improve the sterile process by using the long needle technique. With a needle hole in the lid, water and spore solution can be loaded and unloaded from the jar with little air contact. Spores can be scraped into solution through the hole in the lid with a sterilized long blunted needle or implement, improving isolation and sterility. Extra long syringe needles (heavy gauge 3 1/2 inch - science supply) have their use here. The needles have to be blunted to work correctly at scraping spores off the bottom of a jar. Blunt them with a large wire cutter and finish the tip with a file. These type of needles can be bought, but finding them is the task. If questioned by the seller on what the use will be, just tell them the truth. Mycology is OK . Try your local veterinarian or science catalog retailer. The needle techniques are very versatile and can be employed in many ways good for spore solution preparation without the usual lab setups.
tryingtogrow89
Well-Known Member
it seems they are pretty easy, and quite a bit of info out there on how to do it, i think eventually ill try some agar methods for cloning.
Here is a pic of my Albino A+ inoculated 7 days ago, starting to get real rhizomorphic and spread real quick.
this is my first attempt with growing shooms.
Here is a pic of my Albino A+ inoculated 7 days ago, starting to get real rhizomorphic and spread real quick.
this is my first attempt with growing shooms.
Blackhash
Active Member
What strain are you growing? Didn't catch it.
I have a WBS bulk rezz tray going right now, the strain is PF classics.
I also have a full syringe of Albino A+'s. (got a free syringe for the price of 1)
Then I also have 4 BRF jars going of the PF classics also.
Man I love shrooming so much.
edit: im gonna start a journal on this website once I get them into fruiting conditions
I have a WBS bulk rezz tray going right now, the strain is PF classics.
I also have a full syringe of Albino A+'s. (got a free syringe for the price of 1)
Then I also have 4 BRF jars going of the PF classics also.
Man I love shrooming so much.
edit: im gonna start a journal on this website once I get them into fruiting conditions
Bwpz
Well-Known Member
Definitely link me to your thread when you get it started, very interested in seeing it
And all 24 of mine will be Golden Teacher's.
Bwpz
Well-Known Member
it seems they are pretty easy, and quite a bit of info out there on how to do it, i think eventually ill try some agar methods for cloning.
Here is a pic of my Albino A+ inoculated 7 days ago, starting to get real rhizomorphic and spread real quick.
this is my first attempt with growing shooms.
Lookin good man, can't wait to see em
Thanks for the info on making spore syringes as well, definitely gonna help
Blackhash
Active Member
Are you going to experiment with LC's? They are an easy way to make mycelium that can directly be injected into any substrate and since its already alive growing mycelium, it doesn't need to germinate like the standard MS(multispore) syringes do.
oooh golden teacher. Might have to get a print off you
oooh golden teacher. Might have to get a print off you
Bwpz
Well-Known Member
Are you going to experiment with LC's? They are an easy way to make mycelium that can directly be injected into any substrate and since its already alive growing mycelium, it doesn't need to germinate like the standard MS(multispore) syringes do.
oooh golden teacher. Might have to get a print off you
I'll definitely trade prints man
And I've never heard of LC's, but it definitely sounds interesting. Got a guide/link on it?
Blackhash
Active Member
Sure, heres a little simple guide. You will need glovebox to minimize contam risk. ( I made mine for a little less than $15)
And LC stands for Liquid Culture.
Materials
-Jars w/lids (if you use 1/2 pints use 1 teaspoon, pints use 1 tablespoon, quarts use 3 tablespoons)
-Pure all natural honey (grocery store)
-PC
-Micropore tape
-Syringes
-glove box
-1 empty pint jar "sterilized"
-Alcohol (preferably 91%)
Step 1
Create a lid(same method as creating lids for your jars) but make another hole for the vaccum that gets created while pressure cooking. cover both with micropore tape.
What I do for my lids, is make 1 hole in the center.
Then cover that hole with micropore tape(for air exchange).
You can also use high temp silicone to make self-healing injection ports.
Step 2
Put 1 teaspoon honey into a sterile half pint jar(if you use that, its what I use for now)
After putting in the honey, put in some HOT tap water and fill it a little higher than 1/2 full.
Step 3
*this step is important*
loosen the jar lids so that they can jiggle a little bit, but they should still stay on. (if you don't do this step, your jar/jars will crack inside the pressure cooker)
Now you need to cover the top with foil(to keep water from getting in through the hole in the top)
Now you need to pressure cook for 30 minutes at 15psi.
Once it cools, open up your pressure cooker and take it out. There might be some stuff floating in the liquid and that's normal.
Step 4
You will need to have the following things inside your glove box.
-LC jar
-2 paper towels soaked in 91% alcohol (one to wipe your syringe and one to wipe the lid of the LC)
-spore syringe
Things you will need OUTSIDE your glove box
-Alcohol lamp(I use a flipped upside-down half pint with some 91% alcohol poured in the concave bottom, it makes a clean & steady flame for a few minutes)
Step 5
Your going to want to wipe your LC lid with that paper towel with alcohol on it.
after that, flame sterilize your needle until it is red hot(takes about 15 seconds, but it REALLY gets red hot)
Then wipe with the other paper towel with alcohol on it.
Expel at least 5ccs of spores into the LC.
Then cover the inoculation points with micropore tape.
You may want to label with the species, and the date so you don't forget.
Step 6
Your final step is to start incubating it, and in about 10 days, you have pure mycelium growing inside the LC.
When you want to innoculate some jars or whatever, just shake up the jar so the mycelium gets spread evenly throughout the liquid, suck up some mycelium with the syringe(you may need a lower gauge to suck up some really thick mycelium.) then expel it just as you would with a multispore syringe.
And LC stands for Liquid Culture.
Materials
-Jars w/lids (if you use 1/2 pints use 1 teaspoon, pints use 1 tablespoon, quarts use 3 tablespoons)
-Pure all natural honey (grocery store)
-PC
-Micropore tape
-Syringes
-glove box
-1 empty pint jar "sterilized"
-Alcohol (preferably 91%)
Step 1
Create a lid(same method as creating lids for your jars) but make another hole for the vaccum that gets created while pressure cooking. cover both with micropore tape.
What I do for my lids, is make 1 hole in the center.
Then cover that hole with micropore tape(for air exchange).
You can also use high temp silicone to make self-healing injection ports.
Step 2
Put 1 teaspoon honey into a sterile half pint jar(if you use that, its what I use for now)
After putting in the honey, put in some HOT tap water and fill it a little higher than 1/2 full.
Step 3
*this step is important*
loosen the jar lids so that they can jiggle a little bit, but they should still stay on. (if you don't do this step, your jar/jars will crack inside the pressure cooker)
Now you need to cover the top with foil(to keep water from getting in through the hole in the top)
Now you need to pressure cook for 30 minutes at 15psi.
Once it cools, open up your pressure cooker and take it out. There might be some stuff floating in the liquid and that's normal.
Step 4
You will need to have the following things inside your glove box.
-LC jar
-2 paper towels soaked in 91% alcohol (one to wipe your syringe and one to wipe the lid of the LC)
-spore syringe
Things you will need OUTSIDE your glove box
-Alcohol lamp(I use a flipped upside-down half pint with some 91% alcohol poured in the concave bottom, it makes a clean & steady flame for a few minutes)
Step 5
Your going to want to wipe your LC lid with that paper towel with alcohol on it.
after that, flame sterilize your needle until it is red hot(takes about 15 seconds, but it REALLY gets red hot)
Then wipe with the other paper towel with alcohol on it.
Expel at least 5ccs of spores into the LC.
Then cover the inoculation points with micropore tape.
You may want to label with the species, and the date so you don't forget.
Step 6
Your final step is to start incubating it, and in about 10 days, you have pure mycelium growing inside the LC.
When you want to innoculate some jars or whatever, just shake up the jar so the mycelium gets spread evenly throughout the liquid, suck up some mycelium with the syringe(you may need a lower gauge to suck up some really thick mycelium.) then expel it just as you would with a multispore syringe.
Blackhash
Active Member
Yes. But you need pure raw honey.
There are many ways to do this, including Karo corn syrup and malt extract, but I find that the honey method would be the easiest.
The mycelium should last however long you need it, but if the mycelium starts looking like its going to solidify the LC, just make another LC out of that LC, haha.
I'd say that a half-pint jar of LC should last at least 3 weeks.
But if you did a quart jar, it would last substantially longer, and not to mention waaay more LC.
There are many ways to do this, including Karo corn syrup and malt extract, but I find that the honey method would be the easiest.
The mycelium should last however long you need it, but if the mycelium starts looking like its going to solidify the LC, just make another LC out of that LC, haha.
I'd say that a half-pint jar of LC should last at least 3 weeks.
But if you did a quart jar, it would last substantially longer, and not to mention waaay more LC.
