skunkushybrid
New Member
If you mean am I satisfied that seeds are predisposed to be one sex or the other then, yes... although I have been satisfied of that for a long time before this thread.First page to last. Have you satisfied yourself?
Seed Predisposition
- Thread starter skunkushybrid
- Start date
MsMILFweed
Well-Known Member
Hey, I remember back on Overgrow there was a thread which was about how you could sex seeds (now if this article were true this would be presuming that seeds are pre-determined). I can't remember the exact details, but it was something like if there was a hole/dip in the tip of the seed, or something like a coconut then the seed was either male/female. Sorry.. .stoner brain, 
I can't remember the details 
I had saved the photo, but lost it when OG went down. I've searched over the net since for it, but never been able to find it. Anyone ever seen/heard anything about it?
I can't remember the details I had saved the photo, but lost it when OG went down. I've searched over the net since for it, but never been able to find it. Anyone ever seen/heard anything about it?
skunkushybrid
New Member
Yes fdd has it, although it has proved to be inaccurate.Hey, I remember back on Overgrow there was a thread which was about how you could sex seeds (now if this article were true this would be presuming that seeds are pre-determined). I can't remember the exact details, but it was something like if there was a hole/dip in the tip of the seed, or something like a coconut then the seed was either male/female. Sorry.. .stoner brain,
I had saved the photo, but lost it when OG went down. I've searched over the net since for it, but never been able to find it. Anyone ever seen/heard anything about it?
I find that males will grow tall and slim and the fem's short and squat, they do this right from the start.
The plants sex is NOT predetermined from seed. environmental factors play a huge roll in sexing a plant. It is evolution. survival of the species. Red light will make a plant think it doesn't have enouph time to mature into a budding plant so it becomes a male. its no secret that males flower before females.but it wouldn't suprise me if skunk wants to argue that too. george cervantes and a group of scientists funded by dutch passion seed company proved this theory over thousands of tests. heres what they came up with.
Environmental factors don't begin influencing your plants sex until there are 3 pairs of true leaves.(not counting cotyledons)
1)higher nitrogen levels during the first two weeks of vegging makes more female plants.
2)lower temps increase the ammount of female plants
3)high humidity increases the number of female plants
4)low growing medium moisture increases male tendencies
5)blue light (MH) increases the chances of a female. choose a red light(hps) for males
6)fewer hours of daylight increases the ammount of females (14 hours)
this information was taken from Henk's archives. the owner of dutch passion seeds
Environmental factors don't begin influencing your plants sex until there are 3 pairs of true leaves.(not counting cotyledons)
1)higher nitrogen levels during the first two weeks of vegging makes more female plants.
2)lower temps increase the ammount of female plants
3)high humidity increases the number of female plants
4)low growing medium moisture increases male tendencies
5)blue light (MH) increases the chances of a female. choose a red light(hps) for males
6)fewer hours of daylight increases the ammount of females (14 hours)
this information was taken from Henk's archives. the owner of dutch passion seeds
skunkushybrid
New Member
if you'd read through this entire thread then you would realise that I have argued this point many times... over and over again. The tests were for feminised seed... and even in that, they are still a load of bollocks.
It is NOTHING like having a baby.
skunkushybrid
New Member
Nope, just you don't...
I read the original and full experiment, from start to finish... it was about feminised seed. Read through this thread, you really need to.
skunkushybrid
New Member
That's where you're wrong... plants are very similar to mammals, and in this respect (breeding) plants are more similar to humans than we are to birds.
OMG. skunk i know you read a lot. but maybe your experimentation time would be cut in half if your IQ went up about 60 points. For those people who want to know the truth click the link. page 20 Marijuana Horticulture: The Indoor ... - Google Book Search
skunkushybrid
New Member
So you expect people to wade through a whole book to get to the 'truth'... why don't you just cut n paste the 'truth' for us all to see?
Plants are more like mammals when it comes to breeding and the transferrance of genetic code than mammals are to birds... fact, in fact it's only just been recently discovered.
If your interest in cannabis stretched as far and wide as mine then maybe you would be up to spec. too...
Plants are more like mammals when it comes to breeding and the transferrance of genetic code than mammals are to birds... fact, in fact it's only just been recently discovered.
If your interest in cannabis stretched as far and wide as mine then maybe you would be up to spec. too...
panhead
Well-Known Member
I agree that the whole theory is seriously flawed,i'd love to see a few peer reviewed white papers devoted to this subject.
There has to be some half crazed botanist somewhere who researched the shit out of this i would think.
its on page 20 . you are talking about something different now. either you are confusing yourself or you are trying to confuse your subscribers.
skunkushybrid
New Member
I'm not trying to confuse anybody... just because you are confused does not mean that this is what i set out to do.
mal_crane
Well-Known Member
I may not be a half crazed botanist, but I've attempted to test some of these theories. My findings were a few pages back, but of course skunk shot them down. Didn't you know that mods are always right?
mal_crane
Well-Known Member
It's a little bit of both. He just doesn't want to lose his battle and tarnish his godly repute as a mod. Arguing your point with him won't work either, he'll just ignore you or become a raging lunatic. I even attempted to get him to test my findings himself and give me scientific evidence to prove that my findings were incorrect. All he could come up with were cut and paste links from his own website without original sources.
skunkushybrid
New Member
I don't need to do your silly little tests.
Anyone that has grown more than one crop will tell you that when it grows like a female, it's 99% of the time a female, likewise for a male.
I have my fem's and males picked from just a few days of veg', and so long as the plants are of good genetic stock... I pick right 99% of the time.
I've done this under 18/6, 20/4 and 24/0. Always the same.
Seeds are PREDISPOSED (different word to predetermined, munch box) to be one sex or the other.
Anyone that has grown more than one crop will tell you that when it grows like a female, it's 99% of the time a female, likewise for a male.
I have my fem's and males picked from just a few days of veg', and so long as the plants are of good genetic stock... I pick right 99% of the time.
I've done this under 18/6, 20/4 and 24/0. Always the same.
Seeds are PREDISPOSED (different word to predetermined, munch box) to be one sex or the other.
mal_crane
Well-Known Member
Well here you go Mr. Always right about everything no matter what evidence is brought forth God-like mod. This was published by a group of scientists who tested the theories of environmental factors affecting the sex of cannabis strains. Some real scientific evidence, not the horseshit plastered all over your own site.
Proceedings of the XLV Italian Society of Agricultural Genetics - SIGA Annual Congress
Salsomaggiore Terme, Italy - 26/29 September, 2001
ISBN 88-900622-1-5
Oral Communication Abstract
SEARCH FOR THE GENES INVOLVED IN SEXUAL DIFFERENTIATION OF CANNABIS SATIVA L.
MOLITERNI V.M.C.*, CATTIVELLI L.**, MANDOLINO G.*, RANALLI P.*
* Istituto Sperimentale per le Colture Industriali. Via di Corticella 133, 40129 Bologna
[email protected]
** Istituto Sperimentale per la Cerealicoltiura, Sezione di Fiorenzuola d'Arda, Via S. Protaso 302, Fiorenzuola d'Arda, Piacenza
Cannabis sativa L., sexual differentiation, cDNA AFLP
Cannabis sativa is a naturally dioecious species with heterogametic males ( 2n = 18+XY ) and homogametic females ( 2n = 18+XX ). The sexual differentiation of C. sativa is strongly influenced by environmental factors such as temperature and photoperiod. Anomalies also occur in floral development like the presence of reproductive structures of the opposite sex, or the development of bisexual inflorescences (monoecious phenotype).
By means of optical microscopy, we have identified the earliest step of apex sexual differentiation as the leaves of the fourth node rise on. More than 50% of the samples observed at this stage have indeed developed some floral meristem buds.
In order to identify the genes involved in these earliests stages of the sexual differentiation of C. sativa, we have carried out an analysis of gene expression by means of the cDNA AFLP technique. Apices from the fourth node of male and female plants, grown in controlled conditions were collected, than the mRNA was extracted and used as template for the cDNA synthesis. Double stranded cDNA was digested with the restriction enzymes BstY1 and Mse1. Analysis of amplified fragments obtained using 60 combinations of the BstY+1 and Mse+3 primers enabled us to identify few hundreds of fragments with an apparent differential expression in male and female samples. In order to verify their actual differential expression, these fragments were eluted from the gel and re-amplified with the same primers combinations that were used to generate them. Subsequently they were blotted in double copies and than hybridised respectively with the total cDNA from males and females apices collected at the fourth node. This approach allowed the reduction of the fragments number to 12. Subcloning and sequencing will permit to track back to the genes having a differential expression at this stage of sexual development of C. sativa and that may be involved in its regulation.
Did you want me to rephrase all that so you can understand it in low-level IQ terms, or can you manage to read it?
Proceedings of the XLV Italian Society of Agricultural Genetics - SIGA Annual Congress
Salsomaggiore Terme, Italy - 26/29 September, 2001
ISBN 88-900622-1-5
Oral Communication Abstract
SEARCH FOR THE GENES INVOLVED IN SEXUAL DIFFERENTIATION OF CANNABIS SATIVA L.
MOLITERNI V.M.C.*, CATTIVELLI L.**, MANDOLINO G.*, RANALLI P.*
* Istituto Sperimentale per le Colture Industriali. Via di Corticella 133, 40129 Bologna
[email protected]
** Istituto Sperimentale per la Cerealicoltiura, Sezione di Fiorenzuola d'Arda, Via S. Protaso 302, Fiorenzuola d'Arda, Piacenza
Cannabis sativa L., sexual differentiation, cDNA AFLP
Cannabis sativa is a naturally dioecious species with heterogametic males ( 2n = 18+XY ) and homogametic females ( 2n = 18+XX ). The sexual differentiation of C. sativa is strongly influenced by environmental factors such as temperature and photoperiod. Anomalies also occur in floral development like the presence of reproductive structures of the opposite sex, or the development of bisexual inflorescences (monoecious phenotype).
By means of optical microscopy, we have identified the earliest step of apex sexual differentiation as the leaves of the fourth node rise on. More than 50% of the samples observed at this stage have indeed developed some floral meristem buds.
In order to identify the genes involved in these earliests stages of the sexual differentiation of C. sativa, we have carried out an analysis of gene expression by means of the cDNA AFLP technique. Apices from the fourth node of male and female plants, grown in controlled conditions were collected, than the mRNA was extracted and used as template for the cDNA synthesis. Double stranded cDNA was digested with the restriction enzymes BstY1 and Mse1. Analysis of amplified fragments obtained using 60 combinations of the BstY+1 and Mse+3 primers enabled us to identify few hundreds of fragments with an apparent differential expression in male and female samples. In order to verify their actual differential expression, these fragments were eluted from the gel and re-amplified with the same primers combinations that were used to generate them. Subsequently they were blotted in double copies and than hybridised respectively with the total cDNA from males and females apices collected at the fourth node. This approach allowed the reduction of the fragments number to 12. Subcloning and sequencing will permit to track back to the genes having a differential expression at this stage of sexual development of C. sativa and that may be involved in its regulation.
Did you want me to rephrase all that so you can understand it in low-level IQ terms, or can you manage to read it?
mrskitz
Well-Known Member
well i think you could be right skunk,,,but (this is just a thought,NOT fact) feminized seeds will grow into females coz they were bred to be that,they could turn herm but thats a different story!they deffinatly will be female at first,but regular seeds are not bred to be one sex or the other so maybe the inviroment they grow in does determine what sex they eventually become?MAYBE,,,,kinda like how crocidile eggs have temperature dependant sex determination,,maybe that kinda thing happens with regular cannabis seeds once they sprought,,,just a thought,,,,,
skunkushybrid
New Member
I can understand it perfectly, but it appears that you can't. I see nothing about sex reversal in those words, only a brief mention of hermaphroditism.