mr. childs
Well-Known Member
running these now, too far back in the tent to check, but i must now...
HydroRed's Horticultural Whorehouse
- Thread starter HydroRed
- Start date
thenasty1
Well-Known Member
where do you get those little tubes that you have the pollen in?
smokebros
Well-Known Member
They're called eppendorf / microcentrifuge tubes and can be purchased on Amazon, Ebay, or other similar online retailers.
HydroRed
Well-Known Member
If you are running the FEM's, you definitely wanna check them for balls. I planted the whole pack and almost every one threw balls. I did get 2 out of the 12 that were pretty solid representations of the legit cut. Just way too much BS to deal with to get em between the balls, runts and "crinkle leaf" mutants.
Look for the huge indica fat fan leaf pheno. Those were the ones that were like the cut.
mr. childs
Well-Known Member
i dropped 5 of them, looks good so far, too lazy to check thoroughly last night. i essentially ran them to get rid of them. i have one of those gigantic fan leaf phenos, i'll try to take pics tonight. i cheated & put 2 of each in a 3 gallon airpot with water only soil under cree 3590 cobs.
HydroRed
Well-Known Member
HOW TO COLLECT FEM (REVERSED) POLLEN
There may be other, better methods. This one is mine.
Supplies needed
Razor Blade
2 small pill bottles with lids
Dark colored reflective faced construction paper
Ball Point Pen or any item similar in length with a rounded end (I used a file and it worked great)
My method for collecting:
1. I plucked off the mature sacks from the reversed plant. I spread everything plucked onto a piece of paper towel (or similar) to dry. I chose paper towel because it has texture, and feel it aided in the sacks not getting blown around off the paper as they dried.
2. I dried it for 2 days in 70*F 40% RH
3. Once dry, I put everything in a small pill bottle. As you can see, I didnt use much.
Use the back of a ball point pen (or similar item) sure to crush all the nanners in the mix. You arent looking to pulverize...but just bust open the dry nanners holding the pollen.
4. Once crushed up, cap it and shake the bottom portion of the bottle vigorously for 30 sec or so to shake out the freshly released pollen. You'll see the pollen sticking to the insides of the bottle. Tap the bottom of the bottle on its bottom edge (on a solid surface) in the same spot to get the pollen to fall to one side of the bottom. You'll see it piling up and seperating from the plant material as you tap it.
5. Once the plant material is seperated from the pollen, just uncap it and reach in with your finger and slide out the bulk of the plant material and keep it on one side of the collection paper. Your not gonna get it seperated perfect so dont try to get too much or you will end up grabbing pollen too. You just want to get the bulk of the plant material without disturbing the little pile of pollen you have seperated in the bottom edge of the bottle. Any pollen that falls from this will be collected later.
6. I poured the remaining contents onto the other side of the reflective faced construction paper. This type of paper makes it simple to scrape up all of the pollen with a razor, and hasnt been affected by static electricity (which can have your pollen moving all over the place making it virtually impossible to scrape and collect).
I seperated the plant material with the razor blade and put it back into the "crush bottle". Your gonna have a slight bit of plant material in the pollen still (as seen in the pile on the right pictured above), so I slid the pollen pile around on the piece of paper and the pollen would basically stick to the paper while the small plant material would move. Pour the traces of plant material (and likely some of the pollen) back into the 1st pill bottle that you crushed everything in. Be sure just to let the plant material slide into the bottle because you want the pollen to stick to the paper -so dont tap the paper on the bottle -just let the plant material slide into the bottle.
**It wont look like much pollen is stuck on the paper until you scrape it with the razor.
Thats when you will realize exactly how much is really there on just 1 pass (as seen in the 2 attachment pictures below).
7. Use the razor blade to scrape the pollen clinging to the paper into a pile. Now put that clean, usuable pollen (now virtually free of plant material) into what we'll call the 2nd "collector" pill bottle.
8. Repeat steps 6-7 until no more pollen is being retrieved.
* I did about 4 passes before I wasnt getting enough pollen to warrant these steps any further.
I then put the collected pollen into a small vial, labeled it, and put it in the fridge for later use.
In total, this complete process took me about 35 minutes start to finish.
There may be other, better methods. This one is mine.
Supplies needed
Razor Blade
2 small pill bottles with lids
Dark colored reflective faced construction paper
Ball Point Pen or any item similar in length with a rounded end (I used a file and it worked great)
My method for collecting:
1. I plucked off the mature sacks from the reversed plant. I spread everything plucked onto a piece of paper towel (or similar) to dry. I chose paper towel because it has texture, and feel it aided in the sacks not getting blown around off the paper as they dried.
2. I dried it for 2 days in 70*F 40% RH
3. Once dry, I put everything in a small pill bottle. As you can see, I didnt use much.
Use the back of a ball point pen (or similar item) sure to crush all the nanners in the mix. You arent looking to pulverize...but just bust open the dry nanners holding the pollen.
4. Once crushed up, cap it and shake the bottom portion of the bottle vigorously for 30 sec or so to shake out the freshly released pollen. You'll see the pollen sticking to the insides of the bottle. Tap the bottom of the bottle on its bottom edge (on a solid surface) in the same spot to get the pollen to fall to one side of the bottom. You'll see it piling up and seperating from the plant material as you tap it.
5. Once the plant material is seperated from the pollen, just uncap it and reach in with your finger and slide out the bulk of the plant material and keep it on one side of the collection paper. Your not gonna get it seperated perfect so dont try to get too much or you will end up grabbing pollen too. You just want to get the bulk of the plant material without disturbing the little pile of pollen you have seperated in the bottom edge of the bottle. Any pollen that falls from this will be collected later.
6. I poured the remaining contents onto the other side of the reflective faced construction paper. This type of paper makes it simple to scrape up all of the pollen with a razor, and hasnt been affected by static electricity (which can have your pollen moving all over the place making it virtually impossible to scrape and collect).
I seperated the plant material with the razor blade and put it back into the "crush bottle". Your gonna have a slight bit of plant material in the pollen still (as seen in the pile on the right pictured above), so I slid the pollen pile around on the piece of paper and the pollen would basically stick to the paper while the small plant material would move. Pour the traces of plant material (and likely some of the pollen) back into the 1st pill bottle that you crushed everything in. Be sure just to let the plant material slide into the bottle because you want the pollen to stick to the paper -so dont tap the paper on the bottle -just let the plant material slide into the bottle.
**It wont look like much pollen is stuck on the paper until you scrape it with the razor.
Thats when you will realize exactly how much is really there on just 1 pass (as seen in the 2 attachment pictures below).
7. Use the razor blade to scrape the pollen clinging to the paper into a pile. Now put that clean, usuable pollen (now virtually free of plant material) into what we'll call the 2nd "collector" pill bottle.
8. Repeat steps 6-7 until no more pollen is being retrieved.
* I did about 4 passes before I wasnt getting enough pollen to warrant these steps any further.
I then put the collected pollen into a small vial, labeled it, and put it in the fridge for later use.
In total, this complete process took me about 35 minutes start to finish.
HydroRed
Well-Known Member
Eagerly awaiting to see what you get from the testers! I appreciate your updates. Thats a nice looking F&D setup too
Madriffer
Well-Known Member
I copied a guy on the interweb.Eagerly awaiting to see what you get from the testers! I appreciate your updates. Thats a nice looking F&D setup too
HydroRed
Well-Known Member
Plemon Day 57
These are the strains I should have available in FEM seed by late July:
- "Monkey Juice" (Gorilla Glue #4 x Mimosa)
- "Citrus MILF" (Mothers Milk x Plemon)
- "Purple Primate" (Gorilla Glue #4 x Plemon)
Once these projects are completed, I'll go on to pheno hunt the perfect "Orange Julius" (Mothers Milk x Mimosa) and make some S1's. This has been my baby for almost a year now so Im ready to see it to fruition and take it to the next level.
These are the strains I should have available in FEM seed by late July:
- "Monkey Juice" (Gorilla Glue #4 x Mimosa)
- "Citrus MILF" (Mothers Milk x Plemon)
- "Purple Primate" (Gorilla Glue #4 x Plemon)
Once these projects are completed, I'll go on to pheno hunt the perfect "Orange Julius" (Mothers Milk x Mimosa) and make some S1's. This has been my baby for almost a year now so Im ready to see it to fruition and take it to the next level.
ThatSpudGuy
Well-Known Member
i would gladly pay for all 3 of them crosses, whatever the price. that GG4 x Plemon sounds awesome man
Madriffer
Well-Known Member
1st pic seed mum
2nd rooted clones in super soil
3rd fresh cuts in RW cubes
Once fresh cuts root will throw them in to the 12/12 flood tray.
So far these 2 mums are doing good.
HydroRed
Well-Known Member
HydroRed
Well-Known Member
Thanks Spud! Im working on making them available to everyone right now (even folks outside of the US). Great things are on the horizon, but I'll make that "official" announcement in the next day or so.....
horribleherk
Well-Known Member
nice to see you still at it & thriving I'm gearing up to fire one of my tents back up in a few weeks
Madriffer
Well-Known Member
The cuts root faster than the GG4 I have.
