DIY Thin Layer Chromatography (TLC) of cannabinoids at home - tutorial

Discussion in 'Advanced Marijuana Cultivation' started by PhenoMenal, Nov 16, 2017.

  1.  
    dogcat

    dogcat Member

    thanks man, much appreciated! This thread opened my eyes to the world of TLC, and made it possible for me to participate. I can't wait to do it again on some cbd strains. I'm such a newb, I thought the plates we reusable, I wasted space on that first one. Live and learn!

    By the way, any chance to explain "forums dont use HTML, you'll need to use the icons that are made available when posting for various formatting options"? Thank you
     
  2.  
    PhenoMenal

    PhenoMenal Well-Known Member

    see "BB Codes" at https://www.rollitup.org/help/
    You can either manually type them, or highlight the text you want to affect and then use the various icons just above the textbox that you type these replies in

    And yes the TLC plates are single-use! especially considering cannabinoids from resin are seemingly sticky, and Fast Blue is a staining dye, i'm not sure how easy or possible it would be to get that out of the silica without making an absolute dogs breakfast out of it lol
     
  3.  
    dogcat

    dogcat Member

    perfect, thank you
     
  4.  
    guerreiroweed

    guerreiroweed Member

    Can anybody link me with a place that sells TLC plates and ships internationally?I can find everything easily in my country, but the tlc plates are impossible to obtain, they only sell for juridic persons...
     
  5.  
    PhenoMenal

    PhenoMenal Well-Known Member

    TLC plates, the ones we use in this thread, are simply silica on an aluminium sheet base, so there is no reason why they would be hard to find in your country, and theyre used for science in all countries. Your google search terms might need refining :) try "silica plates aluminium"
    Also, simply try emailing some labs in your country.
    Can probably find on ebay too.
    specifically, the ones i use are German-made by Merck, "TLC Silica gel 60", aluminium, 5 x 10 cm. (no fluoro indicator)
     
    Last edited: Jun 11, 2018
  6.  
    guerreiroweed

    guerreiroweed Member

    I found one, pretty expensive but better than importing, there are 2 options, 5x10 with fluoro indicator and 5x20 without it, can I just get the 5x20 and cut them all in half?
     
  7.  
    PhenoMenal

    PhenoMenal Well-Known Member

    yes, aluminium plates are easy to cut with a regular pair of scissors
     
    guerreiroweed likes this.
  8.  
    cogitech

    cogitech Well-Known Member

    Love this thread!!! Ordered some KOH and going to be trying the Beam's test on some (supposedly) high CBD weed I am ready to harvest.
     
  9.  
    raggyb

    raggyb Well-Known Member

    Ran a Beam's test on 4 just dried strains plus Afghan which I know has no CBD. I decarbed first. I didn't decarb before but it worked both ways.
    [​IMG]
    I could not get a picture that shows what it truly looks like. I ordered them from clearest to yellowest from left to right. But all you can see in the picture is that the one on the left is different from the other 4. As expected that's Afghan and it confirmed it had no CBD. The 4 have CBD and It's just my guestimate, but I believe the order of increasing CBD is Blue Dream CBD, Blueberry, Candida and my Canafdida cross. So two surprises, one being the Blueberry > Blue Dream CBD, and two Canafdida > Candida. Canafdida = (Cannatonic x Afghan) x Candida.
     
    cogitech likes this.
  10.  
    PhenoMenal

    PhenoMenal Well-Known Member

    CBD/CBDA (Beam's doesn't seem to matter if it's decarb'd or not) gives it a red/purple tinge, perhaps there is some CBD in that Afghan afterall. TLC would confirm
     
  11.  
    raggyb

    raggyb Well-Known Member

    I want to do TLC. But I don't know why my camera makes the one on the left look red, it's actually clear. The others are yellow. But I'm using grain alcohol not the other stuff you said, because I have some I don't need for anything else anyway. Maybe that makes the color indications different too.
     
  12.  
    PhenoMenal

    PhenoMenal Well-Known Member

    yeah thats weird if its clear but looks so red on camera! maybe put some white paper or something behind it when you photo. btw a bottle of methylated spirits is < $5 but id guess grain alcohol would work if it's 50+% ... methylated spirits is usually 95-98% ethanol
     
    raggyb likes this.

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