Cloning Plants By Tissue Culture

Bonkleesha said:

i was just reading that root cuttings of varigated plants will never produce a varigated clone. also, was reading its easier for a root to grow chutes than it is for a chute to grow roots. just got my mind spinning.

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never tc'd a variegated houseplant, but my understanding is that the ones that are chimeras, actually containing tissues of genetic make up growing adjacent to eachother, that one would have difficulty exact cloning. however I may give this a whirl on a chimera in my home. the aberrant characteristic is limited to epidermal tissue, which is already commercially cultured this way.

You are correct though, a root snip from a chimera(some thornless berries,seedless oranges,grapes) will not contain both genes(maybe if lucky ??). Marijuana is not a variegated chimera(YET !!) so no worries.But if one did, for instance, make a bioluminescence marijuana plant into a variegated chimera, they could all but guarantee a "clone only " strain, increasing its value immensely I imagine, just sayin.

great idea in the name of corpocracy !

nitro20% said:

thats a question i had on that other tc thread,,,how come all the pics of MJ look like they just got jared,,clean and look just like a potted MJ plant....not the ones of real results though,,,,they look like kinda what u describing! And u never hear from those dudes having pic of a perfect looking, fresh jared culture...i aint scared to show a screw up..or even lame results,,,i-aint-a-lyin-2-myslf.....

I have tryed to find definitions for plant preserve,,but the same thing is found,,,hydrates,,and has a wide soectrum for killing contamination in TC..a must for tissue cultures...I knew this was somthing that was not replaceable,,,and i hope that the 30ml bottle will be here this week!!

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here ya go. I threw in some mold, for good, honest measure. but the shooting/rooting one has been cut and is hardening/or in veg already. maybe i get some pics of hardening if you want? or vegging?
that callus produces clumps of roots and shoots with roots, continually, for me to cut off of. I scalpel the mini plant right off the callus and carefully ,on its side, cut through the roots, to preserve roots with the shoot, into another jar to grow more roots if I have time or straight to hareden/veg. but first, a dip into dutch master Replicator gel and into rockwool in a dome tray.

that ppm is a mystery, but it works. i dont want to know. i dont care. i'll support their biz and allow their function of profitability, because its the american way, and they did all the work.

peace

That problem is easily solvable through in vivo (not in vitro) chromosomal differentiation. Innate parthenogenisis-oriented plasticity methodology is a requirement though.

wow, you smart..thanks.. what does this mean though?

peace

nitro20% said:

thats what i mean, those dont look any thing like plants to me,,,,were is the stem u cut to split...were is the roots? why is it green all the way through ur gel? so many questions..those are real progress pics..not just jared cuttings/bleached and clean...this is actually getting to the point were i might be SCARED....a culture with roots,,the one u might be getting ready to pot..pics.

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cut on the line, transfer the callus into another shooting rooting compound for more divisions. this explant has small roots I cant seem to focus on, beneath the gel. she has lots of time to develop roots in another stronger rooting jar, or dipped in rooting compound and put in rockwool. all my cultures are green, for the love of proper nutrition, including sugar. when roots fully develop in vitro mine need to be lite tight for some reason, so no photos of those, but I shoot some hardening/vegging soon. maybe get a real digital microscope too!

ok, now I see what you've done. the sticks you're starting with need to be scraped, and trimmed to a nub the size of a marijuana seed. look at one of those new shoots on your stick..thats the part you want to start with from the host plant, a new meristem growth shoot, about mid way down the stalk, but grown away from it, like the tip of a branch used to clone. just the very tip of new growth, not the whole stick. near impossible to support this stick, or sterilize it. Currently you are making simple clones in a jar, expensive time consuming ones, but they are clones, and can be dipped into gel and rooted as such. host moms should be clean, dust and mold free before explants are taken, mornings only, just like clones.growth shoot TIPS only, will do what you're looking for. scrape the cambium layer because.....mitosis takes place in the shoot apex (meristem), root tip meristems, and the meristematic cambium layer of the stalk. we using that knowledge to hasten this procedure. kit worked awesome, but I was bought out. sorry.

your pics prove to you that your mix is supporting growth, let them grow and see what kind of growth they show, keep you r notes, yo umay like what you find. I always take alot of cuts from callus, hoping I get that freak someday.also, I found the gene that bioluminecses in plants and animals. its available to me, pretty expensive and so far the bids for my offer are too low.

Where did you find it and where do you keep it currently?

supplier contact, recumbant genes-r-us type of outlet. I'll store the flock in a heated area, um, but I never said I had them yet dude.

as said earlier, anyone can culture bioluminesce genes, some are on raw fish, just waiting to be cultured. not sure if those can be used for anything other than study though, however, the one from the jellyfish has already been used on plants and several animals, fish, cats, rabbits, mice, etc.

pharmacoping said:

as said earlier, anyone can culture bioluminesce genes, some are on raw fish, just waiting to be cultured. not sure if those can be used for anything other than study though, however, the one from the jellyfish has already been used on plants and several animals, fish, cats, rabbits, mice, etc.

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What are you going to do once you have cultured some bacteria that can luminesce? That doesn't really get you anywhere except for having some bacteria on an agar plate.

nitro20% said:

holly shit man thats a lot of words,,,,,i dont think it will go for long..plus i need to get something together now...so scrap all the outer layer off and start with a part out of the nod,,, not the nod itself?mmmmmm just a 1/4 inch of that sprout of the mother nod,,brand new little things..how do you push those WIMPY things into the gel? that ppm tech. store hasnt sent or processed my order yet..they have the order but nothing happened,,,i called but,,their closed...now,,,,I have a desperate feeling,,,that nadcc will not last much longer,,, i can see it wearing off now,,,i cant re-jar them again with out that ppm,,it makes no sense,,,till i think about it!!!! ALL YOU WANNA BEESSSS LIKE ME DONT START WITHOUT PPM!?!@#$#@

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Yes, part of the node, the newest part of it. I dont push them in, I ease them down, and settle them, or make a little piece of paper like a bridge, two sides in, loop up and out, and then set the culture on top of that. I'm jsut starting to play with this suspended technique. evidently they sell jars made to empty and refill without disturbing the suspended culture. might be cool.

WolfZen said:

What are you going to do once you have cultured some bacteria that can luminesce? That doesn't really get you anywhere except for having some bacteria on an agar plate.

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yep, especially if you weren't paying attention in class, tissue culture 101. Then, we eat the bio luminescent material for the glow in the dark poop silly, what else ? nitro might have some ideas, but I'm too busy to play.

nitro20% said:

HELL YEAH. AND THAT SOUNDS FUN TO..GROWING THING FROM CELLS IS COOL......BETTER THEN LOOKING OUT THE WINDOW ALL DAY.....

Ordered a set of 10in. stainless forceps friday at midnight from this aquamarine place 4.95..and they have the bend at the end,,,,,they are already here...WERES MY PPM.....

but,,,, thats cool though huh???that i got one of the hardest parts to start spouting,,,and im doing it ALL WRONG!!!TAKE THAT!!! one for the GRASSHOPER!!!

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those forceps will make things easier for sure, and a good price too ! you may as well keep those cultured clones going, they are possibly viable, and definately study worthy.