Cloning Plants By Tissue Culture

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nitro20%

Active Member
yeah i see where you are at...I thought he meant that IF we put the RIGHT amount,, of the RIGHT things,,, then,,, that plant would FLOURISH,,thought about allllllll the different recipes in TC for every plant....there not the same. WOW ALL the things that i know now......

U ARE RIGHT about NEW nutrients for hydro/dirt,,, IF used correctly,,all the TECH. that we know from TC is changing the way to grow plants (ANY)... changes the way I SEE it!!!

PHARAMACOPING DUDE is there a label on the medium u suggested with a basic macro-nutrient count,,ive looked for days!!! seriously,,thought i saw one that said 10-10-10 n-p-k..and you said to get the carrot medium mix and mix to a no more then 150 ppm,,,right....WELL!!! (I DIDNT GIVE UP)its coming and cant seem to find out how to do that with out a ppm meter.......???:wall:
OR just put the whole pack in a liter??done....STILL WANT TO KNOW A LOW AND A HIGH n-p-k ppm for my average medium..IF anything just a ppm on the nitrogen......have a recipe @ 90 ppm total macro-nuts. IS THIS TO MUCH? IT has 33 ppm nitrogen???? backed out of my math and it was 11.2% more then a seedling.....OR WHATS STRONGER? seedling or a just cultured "culture"

THIS IS WHAT I THINK I SEE!!!! GRASSHOPPERS;-)......... THE CULTURES ARE DOING WELL AND IT SEEMS LIKE THEY ARE PERKY AND WANT TO GROW THINGS,,THE PROBLEM IS,,, THE NUTRIENTS,,,I ADDED ONLY 2 DROPS OF LIQUID KARMA WITCH ONLY IS ABOUT 6-9 PPM OF A SEAWEED EXTRACT THAT IS .1-.1-.5 N.P.K. BUT IS FULL OF AUXINS AND CYTOKININS THAT HELP PLANT CELL DIVISION,,,BUT IF THE PLANTLET HAS NO OTHER MEANS OF SUPPORT FOR GROWTH...WWWWWWEEELLLLL...THIS IS WERE THE LA-BUR-I-TORYS DO THAT WORK FOR YOU,,,THEY KNOW WHAT LIKES WHAT,,,THIS HAS BEEN GOING ON SINCE THE 50s..........


AND THATS WHAT THIS GRASSHOPPER THOUGHT IT SAW WHEN IT JUMPED:bigjoint:
 
N

nitro20%

Active Member
IF YOU NEVER HAD BUGS OR MOLD.... THEN YOU SEEM LIKE A NEWBIE TO ASK HOW TO KILL IT!!!! WE ALL KNOW THAT THERE IS NO CURE ALL FOR BUGS OR WHATEVER...NUTRIENTS,,,SHIT FIND YOUR "THANG" FOR UR "GANG" AND SICK TO THE SAME "THING":lol:
 
N

nitro20%

Active Member
There is so much to learn AND remember before taking on a TC formula...like read ur dudes messages before asking a question!!! ALRIGHT then..now im some where..NO where can i find a dumb dummy ppm for these callus mediums..there secret..thought the secret was in the other things they added... oh well,,, so 150 ppm...one drop quality nute..is about 3-20 ppm..right..RIGHT!!! 3 days 3DAYS!!! I looked!!!!
 
N

nitro20%

Active Member
OK FUKRS!!!;-) I SEE WERE U ARE BOTH AT WITH THIS PHILOSOPHER DUDE,,AND I CAN SEE WERE WE THINK THAT THIS IS THE "END" BUT LOOK AT IT THIS WAY,,,WE HAVE PUSHED ALL PLANTS TO THE LIMIT,,IN A WAY,,RIGHT, TO THE POINT WHERE "OUR" TECH SEES NO MORE OPTIONS FOR FASTER GROWTH OR NOT SO BIG OF A DIFFERENCE TO CALL HOME OVER..RIGHT? WELL THERE WILL BE THAT ONE MORE THING THAT WE WILL DISCOVER,,,THAT WILL HELP A PLANT USE MORE OF A SUBSTANCE THAT "WAS" ALL "WE" COULD GIVE IT FOR THE MINIMAL LAW...WE SEE THAT "THIS IS THE END" 20 YEARS FROM NOW,,SOME ONE WILL SAY THE SAME THING..."THAT PLANT CANT GROW ANY FASTER" I DONT KNOW BUT EVEN THE "THEORY OF RELATIVITY" IS IN QUESTION!!! SAME WITH HUMANS,,,WE'RE ONLY AS STRONG AS OUR WEAKEST PART,,, IQ:-P, BROKEN LEG, NO DRIVERS LICENSE, A WIFE:cuss:,E.C.T. ALL THESE THINGS CAN CHANGE IF WE ADD THE RIGHT "OTHER" THING!!!!!I LOVE TO THINK
 
pharmacoping

pharmacoping

Active Member
[h=3]16 oz. Ecogrow Standard (10-8-14)[/h]Ecogrow Standard (10-8-14 N-P-K) was developed for the Pacific N.W. Climate (temperate zone). It is ideal for our most popular hydroponic plants -..


ms carrot culture will arrive at around 150-200 ppm, if its a one litre kit. more will kill. hormones just make more things happen, at varying speeds, not the nutrients, assuming they are well balanced and complete. I'm guessing that liquid karma alone would not grow a healthy plant through its cycle(but I dont know). if thats tru, then it wont hellp in tc either. use eco nutes (period) or buy pre mixes like murishage and skooter for carrots.

if you use basal salts, you must have a ppm meter. hanna, 29$ amazon. batteries last for years, my hanna is 15 years old and cost me 39$. ph paper is amust also...6.5 is good. adjust before you hormone though. if food is right, your growth speed is caused/limited by the hormones used, hosts original health, and contaminants in vitro.
 
wyteboi

wyteboi

Well-Known Member
guile an pharm , thank you very much for the info ..... dont worry you didnt scare me away .....yet , i just been a lil busy. im readin every word and am gonna start testing in a few months , once i get enough knowledge to at least try.




soil
 
pharmacoping

pharmacoping

Active Member
wyteboiiiiii !,

thanks for stopping by man, cant wait to see your creations ! I threw eggs(fresh, from chickens, even, not sure which came first though, dont ask) in a jacket pocket this morning, with my digi camera, and one broke ! otherwise I'd end this note with some cultured cuties. I think the cam will be ok.

peace
 
N

nitro20%

Active Member
got fresh eggs too...man oh man,,,i eat 2 dozen a week,,of GOOD chicken eggs...4.95 a dozen...it gets expense...


i read back through this a little,,,some where on the rule list is: dont over think it!!!

and maybe that simply hydroponics you tube vid. may have a working mix...IM sure glad nobody reads this shit!!! if u go on the net.. and start lookin for a tc recipe/dip n grow you will end up seeing these threads all over...


no... liquid karma can hurt u with to much/with no n/p/k..it is just a general additive... starting to see another way of looking at plant growth...
 
pharmacoping

pharmacoping

Active Member
the nutrient mix is a no brainer. quit thinking about it at all, buy the basal salts, or a muri skoog mix and do it right without a thought. You could mix ANY complete hydro nute, very weak, under 200ppm, and it will work well !!. if its got iron, a multivitamin works great even. the hormone mix will need your duty.

we keep free range chickens forever. we dont eat the chix, but we do breed and eat/gift the organic eggs. if people knew what an egg went through to get to their plate, they would do the same I think. rabbits to, got a buck and two does, they very intimate, when visiting eachother, but we do eat their young, right after they get off the nipple !, for free, without feeding them. they'll never taste any better, and we have hundreds in a couple months if we want.

for the cost of three dozen eggs, you could have all the hormones you need for a year you know......
 
Bonkleesha

Bonkleesha

Active Member
i was reading somewhere that someone was crushing up a B-vitamin and throwing in a drop of superthrive with good results. i wonder if he was using an antibiotic.
 
pharmacoping

pharmacoping

Active Member
I used to use a multi, before basal salts were available in the 70'ss-80's, did have results of live tissue, but would never calll them good results. no results using just those for nutes have ever produced anything that left the culture vessel, that I know of, or ever seen. the culutre may live for sure, but will not yield any of the token results we're looking for, except a chunk of callus materiL, OR A SPROUT, FOR OBSERVATION.
 
pharmacoping

pharmacoping

Active Member
plant preservative is a given, a standard, and should not be given a second thought. just get some, use it, and never look back. I dont know what it does, but I cant operate my way without it. most bio hackers use it in vitro for better results.

explants will never look like mj, until hardened and planted in medium outside of the vessel. they are bonzai's , I think due to lack of photosynthesis. hope that helps

Big Tom Callahan: Of course, I can get a hell of a good look at a T-Bone steak by sticking my head up a bull's ass, but I'd rather take the butcher's word for it.
 
pharmacoping

pharmacoping

Active Member
dontexist21 said:
How long can the root balls be stored before you are forced o put them in veg?
Click to expand...
I'm never forced to put any in veg. my rootballs thrive indefinately(can be divided over and over too) after a month or two sometimes negative changes can be seen, suggesting it be transferred into a freshly mixed vessel. The root ball continues to grow, so theoritically it could outgrow the vessel too.
My rootballs are stored until they need to be sprouted, then hardened off then to veg.
 
pharmacoping

pharmacoping

Active Member
yes I have(hundreds)in vitro, to complete harvest and use. The biggest difference I see, which is huge to me, is a uniformity thats lost eventually when traditional clones are taken, and a very pure, more pronounced growth,pattern,terpine profiles, without any kind of issues, including pests, and no knots on the stems, so I think they are super girls, caused by a purified dna, free of any replication infection/dna changing virus. also, the vegging is much more efficient, due to roots already being present at sprouting, and tighter foliage, ending in a sort of self pruning action, as all but the fan leaves are gone inside the tight flowers. lab results show consistancy on the tc plants not seen with clones/seeds of the same variety.

but I think you're talking about cloning from a root snip sor so? I have tried this too(hundreds) with (hundreds) many failures, and some success. but again, success is what? I saw sprouts of plants that grew slow, were obviously infected, and yielded very low. not sure, but I think the reason is because those roots, without the protection of photosynthesis, or a sterile environment, are susceptible to disease. And, sugar must be provided, as there is no photosynthesis without leaves, and sugar in your roots/medium come with all sorts of problems,pests/disease unless sterile/in vitro.

when harvested, if you leave one tiny branch at the bottom, and a bit of the thick stalk cut correctly, and vegged again, will result in another full size plant soon, complete with multiplying problems that may or may not have been evident in the first fruiting. they often look like a clone taken from a "topping" of a plant, which is not ideal, at least for me. this area has the most infectious contaminated material, right down to the dna, of the whole plant. thats why they appear differently.

peace
 
Bonkleesha

Bonkleesha

Active Member
i was just reading that root cuttings of varigated plants will never produce a varigated clone. also, was reading its easier for a root to grow chutes than it is for a chute to grow roots. just got my mind spinning.
 
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