Cloning Plants By Tissue Culture

nitro20% said:

yeah im not agrueing,, AT ALL,,,,I like the 3 drop thing,wish i had a ppm meter...right,would that be helping me...
so i wonder when the contamiates will show up,,,tonight,tomorrow,,beginners luck @ 100%

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Hope you don't think I'm stressing, I like these sorts of things... its interesting to look back and see where I make my mistakes. I just hope your not getting too frustrated or feeling talked down upon, there is no intent for that here (but I know how it can feel when its hard to read the language)...

A PPM (TDS) meter might confuse things further at this point... They work by converting the electric conductivity into a ppm (or Total Dissolved Solids) reading. The odds are that your Dip'n Grow will not read 15,000ppm (10,000 IBA plus 5,000NAA) and the water you use to dilute it is unlikely to be 0...

We are just working with weight and volume here.. We assumed that the Dip'n Grow had a specific gravity around that of pure water 1.0 (thats 1 kilogram per liter). for comparison sake 100 proof alcohol has a specific gravity around 0.9 and "Kool aid" has a specific gravity around 1.1 (think about how much sugar you use to make that crap)..

Assuming the Dip'n Grow (concentrate) had a specific gravity between that of 100 proof vodka and Kool aid (say the specific gravity of water, 1.0) we speculated that 1% of the 1 kilogram that a liter of Dip'n Grow weighs should be IBA weighing about 10 grams (within a 10% margin of accuracy).

Latter we were validated when the mixing instructions were brought to light.. They clearly state that there is in fact 10,000ppm IBA in the undiluted Dip'n Grow concentrate.. This means that it has a specific gravity of 1.0, that there is in fact 10,000 miligrams (or 10 grams) of IBA in a liter of Dip'n Grow weighing 1 kilogram (1,000 grams).

Now its just a matter of figuring out how many parts containing 10,000ppm (10,000mg/L) we need to dissolve in a larger volume of water to obtain 1.5mg IBA per liter (1.5ppm).

We know that a "drop" is considered to be 1/20th of a milliliter, and a milliliter is considered to be 1,000th of a liter (There are 20,000 drops in a liter.).

If we wanted say 1ppm (1mg/L) IBA and our concentrate is 10,000ppm (10g/L) we would simply dilute it to 10,000 times its volume with water (our solvent of choice).
So 1 drop of Dip'n Grow to 9,999 drops water (to make 10,000 parts total) would give us 1ppm (1mg/L) IBA. We know there are 20,000 drops in a liter so 10,000 drops would be 1/2 liter (or 500ml)

But we want 1.5ppm (1.5mg/L) thats 50% more than 1.0 so if we doubled the water but added triple the concentrate we would get a 50% stronger solution making 1.5ppm (1.5mg/L)..

Thats 3 drops per liter...

I think I might keep confusing the issue by giving you the 1ml per 7 quart thing but what I am really saying is 1ml per 6.666 liters. The thing is that 6.666 liters just seems like an odd measurement to work with (I can see someone using more than one measuring device in an attempt to be accurate) 7 quarts are equal to 6.624 liters which is only 42 milliliters off (that's better than 1% accuracy) If you realy wanted to be a stickler you would just add a teaspoon extra water to each quart, and another one for good measure (but now you are using a second measuring device again, which kinda defeats the purpose. Besides do you really need to be under 1/2 if 1% accurate?)

I have GOOD news from the tc thread>>>>as of 72 hours of culture, i see growth of 1/8 to 1/4 inch on all 15 cultures....and no signs of contamination,,,mediums look smooth with a tiny bit of liquid on top,tiny bit.one has a "rough" look,, but it shows no signs of contamination as of yet,,culture is growing...every thing is going BETTER then i expected..ive only entered the area 5 times in 72 hrs..alcohol misting all the way..the tape i used isnt the best..got to get REAL seal tape i guess..planning to culture more when agar arrives. (today)HHEEELLLLL yah!

i see the same thing as you do,,,i saw two ways to look at it,,,your way i understand and is correct. but all the math i did and every thing i thought i read,other then the matrix break down,says 1part con. is diluted by say,,, 4 ml. water, to repeat the exact doubling process i must and 4ml. more water witch gives me 8 ml,,, to the now to break that down double again,,, i would just add,,, i thought,,, 4ml. more water,,,,,not 8ml as i would have from the last...now you say,,take the 8ml. and add 8ml more,gives me 16ml.,,,then add 16ml. to double the original consentrate @ 32ml to the one part we started with,,,, witch intern breaks the PPMs down to what we are after...add that is what the dilution equation is not saying on your behalf,,, as my 1 IQ understands it m1v1=m2v2... maybe im doing the math wrong,,but u tube has step by step instructions on chemistry solution dilution math..in dummy terms,,haha maybe i need STUPID DUMB DUMMY terms.


but thats it though... 1.5 mg/l of 1% iba,, in a solution that is 57.7 g (57700ml/g) total v/m,,, to 1000ml. parts /water ,,,u will need 2.5 to 3 drops of said consentrate....to achieve 1.5 mg/l or 1.5ppm iba...

oh I GOT PICS IF ANY ONE WANTS TO SEE THEM ..I MUST E-MAIL THEM THOUGH .

sweeeet !!! so happy to hear that marijuana tissue culture is possible at home, for more people everyday !! imagine the progress in a few years, now that this mystery is being solved by us stoner alchemists. Pretty sure they try real hard to make this look near impossible, as indicated by many comments made here. I wonder what corporate benefits they may have by keeping this tech out of our hands? govern-mental process

pharmacoping said:

sweeeet !!! so happy to hear that marijuana tissue culture is possible at home, for more people everyday !! imagine the progress in a few years, now that this mystery is being solved by us stoner alchemists. Pretty sure they try real hard to make this look near impossible, as indicated by many comments made here. I wonder what corporate benefits they may have by keeping this tech out of our hands? govern-mental process

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What kind of mixtures have you used to make your gel medium for cannabis cuttings? what were your experiences with them?

HEY EVERYONE!!!HERE IS ANOTHER UP-DATE FROM TISSUE CULTURE 101 (tc101inprogress)
THE BEGINNER!!! i guess thats what we're doin.. for all of YOU that skip over the entries to find the latest(me to),,,heres the deal...I decided to study this, for the knowledge, 3 weeks ago...as of 80 hrs ago, I TCed my first ever cultures..I cut all green leafy material from 19 pieces of branch/nods....i have witnessed the growth of 1/16 to as much as 3/8 inch on all cultures..no signs of contamination as of 1 hr. ago one culture seems to be darkening a little, but i still see growth..still not sure when contamination shows its ugly head?..someone here does though,,im sure? i am not a beginner farmer,,as it may seem,,the last few weeks made me wonder how those big ass pumpkins even lived or those Fresno peppers,,those colorful fukrs are HOT/good taste too..
i believe that TC may have a huge affect,as it has for the SEASONAL PLANTS to be sold yearly,to the FOOD GROWER or one who like farmers market where one/I might be able to supply a whole bunch of "pure" vegetable plants or such..no more GOV. genetically modified shit that goes through our nation one way or the other....given one might have to find seeds from natural/organic food store to ensure of not getting a modified seed to start with,,but after that one study finds it possible to make 1,000,000,000 plants from 1 in 1 year...even at half that a number of people "COULD" start a whole new place or places to get pure 100% FOOD (that shit sounds like a movie)...personally i eat ONLY food that i can read,,(i miss restaurants)but MY OPINION.and if you read back a little,,,dont look like im surrounded by a hole bunch off IQ's,,damn there goes the last ONE!

i've try and built everything on the market, seriously i have,,,,except for all the 1000000000000000 new cloning gels and things. i even had a version of that cloner every body talks about,,7-8 yrs. ago...and i even updated it to what these new ones look like,,but the look of it didnt help...
i have my best results,,to were i only have to take what i need to fill one of those kiddy pools.(they make great plant base).and i use some sort of pellet,clean not jiffy or something from pay-less.work better i think.. then snip,dip into Olivia's cloning gel and with the pellets soaked in a (im not saying it) a SOLUTION that contains 1 gal. water and about mmmmmmm bout 1 1/2 tsp of liquid karma.i might put less if its dead winter due to to cold weather,, i find that just a pioneer jr. in a oak cabinet no pad, and 10 cfms.(fan modded)they want to purple up on me,,u know what that means...me it means "clones" plus "veg./flowering" divided by "JUST for personal"= not worth my time/energy.
oh and i use bleach on ALL things use to cut or store clones every time around.i hate being so clean some time, it make pot taste like bleach after.wish i knew cleanness 15 yrs ago.

so i was om the web dddduuuuhhhhh,,,, and typed in dip n grow ppm,,know were it took me HHHEEERREEE!! WOW!!! dip n grow is getting more of a name for itself. so lets help,,"i like to help" (fedX)ha ha ha remember that comedy with steve martin,,,
Having results, like this, may need to be heard,,there's not some GRAND BREAKTHROUGH that will make u a $1,000,000,000 in 10 to 14 weeks indoors HAHAHAHA.(thats the newbie goal,,,, right,RIGHT) but for some it might be a new thing to smoke on and maybe play with...with that in mind,, if u search this thread u may find allot of "man, what do they think they are changing" ora,,or math problems (ignore all that). i heard about TISSUE CULTURE 14 yrs ago,and the guy made GOOD acid ,,,and the shrooms,,,wow! I heard what noise came out, but never listened,,,,,80 hrs ago i started the process of "tissue culture" by way of Micro-Reproduction,(just as a,,,, lets see if i could, project) I did not by a kit,and with a little research found that i could BUILD MY OWN KIT (sorry kit and gel makers,,no hard feelins) all of the things can be bought at one huge all in one store..i try to stay out of "THE WALMART" kind of stores!!!!we have to stand up for something...hears a list of what i used and the medium recipe..again,,sorry kit and gel makers, Any how, after research kits was not the choice for me.

-canning jars/plastic lids, and a few extras, pressure cooker, all the items to cut hold plantlets and seal jars properly,all types of ways,, tote or a fish tank/plastic works youll need it for the clean
GROW area.i dont know but a hepa filter will help CLEAN air and better the odds. just think about what u cant see that make u sick,im sure that you've already done some research at this point..So you know bout cleanness and lighting and cleanness & cleaning supplies,,get the hint.
this is basically a med. that,,, with no experience,,, came up with and as 82 hours ago their all growing, with no signs of contamination:

all per ml. and stuff,, is dedicated to guile!!!HAHA even though I went with my last second thoughts.

-mix pool sanitizer (NADCC/plant preserve) @ 1/8 teaspoon per 750ml,,this will be your rinsing and cleaning solution for ur cuttings.unlike bleach,this will not have to be rinsed off before you transfer,although i mixed very little consentrate to get the cuttings to the place of culture.
dont think this has storage life,chuck it when ALL done.
-fill 1000ml to 500ml
-add 30 ml + of your NADCC/ppm note:i added 30ml but will try 5ml/L. more next time.
-add 15 drops dip n grow,,, this will be your iba/naa nutrients for shoot production.
I might have added more then intended,1 to 3 drops was to verdict
-2 tablespoons sugar
-add 500ml water to make 1 litre.
-and your agar agar,, i used flakes at the lables instructions.
in that order
-this is a part were things may fall in ur mix,,so cover it, open the edge dummy,,,and microwave on high.get to boil then try to a just so you dont burn it. i think my first batch burnt,i chuck it...
you can tell when you've cooked it enough,,,common sense,,ull need that.
at this point fill your dishes TRY NOT TO REACH OVER THINGS!shave you arms haha.lids on and to the pressure cooker,,lids lose,tools and things too...

well if you dont know what to do after you pressure cook then "stop" you wasted money, u must study WHY this is preformed before before YOU PREFORM it.or whats the point. Keeping a clean story short,cut nods,some to all "leafy material",3/8 to 2 inch pieces and plant in your chlorine jars,,i saw things happen within 10 hours!SERIOUS.
i have pics but my puter is putering and that aint happen.ill email them though! iaintalier', its true, this is highly possible in your own home/kitchen...my man, pharmacoping,the starter of this high jacked thread, knew it all along, and if "I" can do it, HEAINTALIEIN' either!!!!!!!!!!!!
heres what im basing my info from:http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf

guile,

I've used all the above that nitro used, with many different results. over the counter carrot callus recipe works to get you started. when ready for a change, cytokins and auxins are added at tiny varying amounts, until your desired results are achieved. I never fussed with the math much, and didnt use pool sanitizer(awesome nitro) as I always had acess to the culture wares in the last5 yrs. but before that.....
I've used store gelatin, crushed vitamins, antibiotics, colchicine,bleach,different types of sugar,crushed and sterilized foreign pant material, crokus bulb extracts, banana peels,cloning gels,bushmaster type additives,pk's,salts, etc. lots of successes, and many freaks wanting to break out of the jar, some grew tall, some bonzai, some had barely any leaves,some with dusted calyx clusters(think dr grinspoon). I dont want to manipulate any other way(for now) and just keep producing pure callus material for the mj community, the ones in the know.
I'm not doing it the right way, just my way. we can see several applications in the thread already. I grow callus and rootballs,purify strains, dr tissue cultures perfected clones...and nitro is going to show us "roses with thc" soon !!! lol

Do either of you guys use any kind of nutrients or B vitamins?

I like the NADCC tip too.. Next time I'm in town I'm going to stop by the pool place and pick some up.. Have you tried using it in the place of peroxide (or enzymes) in your reservoir?

You have better experiences with Olivia's gel, peat pellets, and liquid karma than anything else? Do you use peroxide, enzymes, or that NADCC.. Who about B vitamins?

I have to agree about the kiddy pool, I useto do that back in the day.. Best damn flood tables/reservoirs you could buy for $5-10.oo apiece. And they get you decent light distribution with homemade aluminum flashing parabolic reflectors too (took the stitching out of an umbrella once to use as a pattern because I didn't want to figure out the math). To use with the reclaimed oil cooled vertical HID bay lighting out of factory's and such (that was before I started building my own switchable ballasts using sodium rebuild kits and switches).

Do you fill yours with aggregate, like crushed brick or screened gravel? I remember once using 1gal pots to try and get about 25 plant per square meter spacing, (putting clones directly into flowering as soon as the roots broke through the peat pellets)..

You ever make 5 gallon buckets of wine to place in the corners of your white washed paneling "gorw room" around your swimming pools (for Co2 enrichment)? Every 2 weeks I would start a new one. Usually apple, grape, lemon, and tea.. Bummed me out when they started marketing "hard lemonade" and "twisted tea" that could have been me (mine weren't carbonated though). After the first couple weeks fermentation would slow down and by 8 weeks things were usually ready to bottle/drink.. I had a pretty ghetto 1 gal still too, made a couple glass of brandy at a time though .. To be young and creative... I've gotten so square...

SO it been 4 days and i see that 2 jars containing 3 plantlets (2/1 and 1/1) might be not doing well,,not concerned YET no sign of contamination...note: NADCC only will have its benefits for about 72+ hours..plant preserve material has a wider scope for TISSUE CULTURE .. growth IS noticeable, essentially in the widening of the stem,,jars look good.....
-----important message------(error0425)-----important message------
AFTER CAREFUL CALCULATIONS AND A KICK TO MY ESTEEM,,,THE RIGHT DILUTION IS 5/16ML PER LITRE OR 99.8% SAY 20 DROPS IS A ML.,,SO AT THAT IS...3.2 DROPS OF DIP N GROW PER LITRE IS 1.56 PPM..
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Im not seeing the growth that i dreamed i read about,,but i know from every thing i read,the harder wood plants that are in lower ppm of iba do much better.
1-2.5 ppm iba with the naa at .5 lower.(dip n grow 1v/w iba and .5v/w naa) but at 13to15 drops,,there still growing..
yep,, auxins and cytokinins,, haven't found a source that i could measure haha. but ive looked into willow tree water (got that) and coconut water,but if you know something better/easier id love to here it. that,,, i will need to add in a few weeks. `
I dont know bout thc roses,,,im sure stranger things will happen,,like,, 60 miles south,I-5 corridor,,its snowed once,ski park,, no go,,some wonder if its cause they complained enough,,the gov. finally let it snow on em, ONCE!! couple feet.mmmmmmmmm

Nope i thought about using willow tree water,,but i never knew what was in it..now i think that i will use dip n grow to soak my peets,, all the hubub about the cloning gel/powders that's all that's in them is auxins and cytokinins.
i put 1 gal pots in there transplanted from clone cab. then 1 1/2-2 wk.veg/then flower,,same thing..nothing huge,,but it supplies my demand for bout $100 in electric/month.i smoke ALOT!!!!AAALLLOT!!!!!!!one of those raptor hoods would over it be sweet the 1000 hortiluxes (hps/mh) would love it.. maybe soon,, local dude,,, $200.00 for the, made in the U.S.A. one.
As for any thing beyond basic n/p/k with high Ps at bloom,, nope,, consistency defines ALL failure..Im not saying that there is no benefit to all the (tech stuff) there is some excellent mj/and things...hormones are auxins and cytokinins,,and the b1 and thingslike "super thrive" i thought correct me though.please!
sure seems like alot of people high as fuk for there to be 1 right way, pharmacoping? I glad theres more than one way...

Guile,
I dont add vitamin b or superthrive or over the counter nute mixes. Eco has pure basal salt nutrients, with msds, since the seventies. I've used nothing but Eco nutes for......ever, until 3yrs ago and swithced to dutch master gold line, nver looked back either.Nothing wrong with pure salts, but..DM makes life and exposure much easier.
These eco salts contain every major and minor ingredient necessary, without additives or hormones. I use a tsp of these salts in my litre mix of agar. My nutrient ratio has not changed in vitro since I switched from a crushed vitamin to Eco salts.
s
My largest reservoir(100 gallon) is a big round plastic kiddie pool too !! every tote I've used breaks down (from the nutes, I guess, as there is no uv exposure under trays) and the pools work great for 20 bucks !

I used to use willow water for cloning. it's salycilic acid and the branches were chewed historically for ions, for pain management. if discovered today this acid has so many uses it would be a miracle acid. Replicator(DM) roots clones and makes a killer foliar for seedlings too, faster than any willow stick I've soaked. I use the Rep now exclusively when I clone traditionally

Ive had my pool for 3 years and i cant tell you how many cycles have been through there.....cleaning with bleach every time,,that thing is still like new..way cheaper then 24 plant bottoms...

cool,,but do use rep.for ur auxins and cytokinin? or is that ur secret...what are vitamins compared to n/p/ks..or is that the deal ,, salts and organics.Are the p/ks the ones im looking for to achieve my goal for the nexted medium?

I buy the diluted hormones as necessary from various online suppliers. I used to use cloning dips for tc, but not in awhile, or with the level of success I was looking for, only a training exercise for me. check out the pure salts at Eco online, they're cheap too. mixed and ready to go, and pure.
You're looking for a weak version of exactly what you would use for tender seedlings, all the way through tc, only hormones change. sugar is constant also, as there is very little photosynthesis happening for carb production. you really should have a 30$ hanna ppm dip checker and start with RO water. otherwise months of trial and error could be yours.

nitro20% said:

SO it been 4 days and i see that 2 jars containing 3 plantlets (2/1 and 1/1) might be not doing well,,not concerned YET no sign of contamination...note: NADCC only will have its benefits for about 72+ hours..plant preserve material has a wider scope for TISSUE CULTURE .. growth IS noticeable, essentially in the widening of the stem,,jars look good.....
-----important message------(error0425)-----important message------
AFTER CAREFUL CALCULATIONS AND A KICK TO MY ESTEEM,,,THE RIGHT DILUTION IS 5/16ML PER LITRE OR 99.8% SAY 20 DROPS IS A ML.,,SO AT THAT IS...3.2 DROPS OF DIP N GROW PER LITRE IS 1.56 PPM..
----------------------------------------------------------------------------------------
Im not seeing the growth that i dreamed i read about,,but i know from every thing i read,the harder wood plants that are in lower ppm of iba do much better.
1-2.5 ppm iba with the naa at .5 lower.(dip n grow 1v/w iba and .5v/w naa) but at 13to15 drops,,there still growing..
yep,, auxins and cytokinins,, haven't found a source that i could measure haha. but ive looked into willow tree water (got that) and coconut water,but if you know something better/easier id love to here it. that,,, i will need to add in a few weeks. `
I dont know bout thc roses,,,im sure stranger things will happen,,like,, 60 miles south,I-5 corridor,,its snowed once,ski park,, no go,,some wonder if its cause they complained enough,,the gov. finally let it snow on em, ONCE!! couple feet.mmmmmmmmm

Nope i thought about using willow tree water,,but i never knew what was in it..now i think that i will use dip n grow to soak my peets,, all the hubub about the cloning gel/powders that's all that's in them is auxins and cytokinins.
i put 1 gal pots in there transplanted from clone cab. then 1 1/2-2 wk.veg/then flower,,same thing..nothing huge,,but it supplies my demand for bout $100 in electric/month.i smoke ALOT!!!!AAALLLOT!!!!!!!one of those raptor hoods would over it be sweet the 1000 hortiluxes (hps/mh) would love it.. maybe soon,, local dude,,, $200.00 for the, made in the U.S.A. one.
As for any thing beyond basic n/p/k with high Ps at bloom,, nope,, consistency defines ALL failure..Im not saying that there is no benefit to all the (tech stuff) there is some excellent mj/and things...hormones are auxins and cytokinins,,and the b1 and thingslike "super thrive" i thought correct me though.please!
sure seems like alot of people high as fuk for there to be 1 right way, pharmacoping? I glad theres more than one way...

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I don't think that many people realize that to be successful (not just at growing either) you only need to be resourceful, otherwise spend a bunch of money... The only thing better than seeing "made in the USA stamped on something is a complete lack of a stamp (because you made it yourself). If you can do it out of recycled/repurposed stuff you are saving the environment to boot. Biulding your own stuff is environmentally friendly in alot of ways, think of all the logistics that have circumvented (transportation = poor air quality and depleted natural resources). Every time you reuse/repurpose something you have cut out at-least 3 round trips (some of which might be to the other side of the world). Not to mention the damage to the economy caused by shipping your money over there... Think locally for the sake of the earth globally...

What nutrients do you use? I useto use Shultz or Peters "complete" fertilizers and add Epsom salt to for extra magnesium that pot plants need... I switched to the GH "flora series" using only the "micro" and "bloom" but have considered changing back to powdered nutrients again as I am spending nearly $1 a day on nutrients (despite buying in bulk and recycling as much as possible). From what I understand anything that works for tomato's (like the GH series) should have high enough magnesium so you don't need to add Epsom salts. I haven't been lately, but I've been using KoolBloom which has magnesium sulphate (Epsom salts) in it.

By the way I am incredibly unclear on what goes into most rooting gels/powders other than the auxins IBA and or NAA. I have been trying to sort out the best possible rooting solution I can come up with, and I am quite certain it will contain a bit more.. I think these culturing mixtures are an excellent place to start... as you are doing essentially the same thing, just on a different scale. None the less I plan to add Superthrive (B vitamins) to my mixture and maybe potassium sorbate (@ 150ppm or so) to my mix (I have a bit of it around for preserving meat and wine) otherwise something else to keep yeasts and molds down...

If you are trying to promote "shoot development" its my impression that you want to be working with both Cytokinins and Auxins, not just Auxins... If I were to guess it would probably be something like BAP and NAA... From what I understand NAA alone will not aide in rooting as much as IBA alone However NAA is more active in other plant development, making it more favorable for shoot development.. I have often wondered why they are not commonly used as a trio, and I'm still a bit unclear (perhaps getting a cutting to do more than one thing at a time increases the likelihood of failure).

It seems that when trying to provoke a plant to develop both new shoots and roots its often done in 2 stages... First shoots, using predominantly Cytokinins and Auxins then transplant to new medium for developing Roots using Auxins (and sometimes Gibberellins).

Yep Im seein that one little drop more/less may per litre changes ALOT of the values of the medium. they started out like things were goin to happen,,but maybe a few are not growing so much at a time....I had this feeling it was to much iba,,,but im goin to make up a new batch here soon and with the right amount this time,and transfer 4 plantlets to new medium and do some other cultures,,(veggie seeds)...so a drop wouldnt, hurt of this eco or of a o/1//1 hydro nutrient additive...
AND my agar powder says 2 tbls per 1 pint about a half litre right? would you use this amount in your mediums,,,cause my readings say about half that in TC.. your opinion...

Had a thought on a new KIT... ALL is in there. tent,lights,cooker,tots,etc,, just like hydro did when it went public....I was 17 in my first appt. (with a 19 yr.old chick)22 years ago,,put a seed on my fish tank and it sprouted,,the next issue of high time had a article on HYDRO,,kidda like what we're doing right now...but im not just going to think about it this time...objectives/goals=SHOT ASS LIFE!!!

yep,for some reason i just cant stand the wastefulness i was teached my whole life!! fukin schools/intsitutions...shit head lie'in gov..its not my fault that the world hates us,,,i just figured it out...theres nobody really helping the USA thats not in it to GAIN SOMEHTHING PERSONAL.Its not their fault either,they got lied to, too!

I use the GH flora series,with liqiud karma,,i use to use all organic (to slow indoors/$),,this is way faster,, and easier,,with the temp change here(day/night),you got to watch what soil/nut. u use..fungus nats are my little freinds..,fly tape tape helps me warn the others,,,ha ha ha..I save the $ for organics for the outdoor crop... im glad that i studied this, cause there is alot of interesting things that makes cloning alot easier,,,this is one of those things that Will always be LEARNIN' me.

nitro20% said:

Yep Im seein that one little drop more/less may per litre changes ALOT of the values of the medium. they started out like things were goin to happen,,but maybe a few are not growing so much at a time....I had this feeling it was to much iba,,,but im goin to make up a new batch here soon and with the right amount this time,and transfer 4 plantlets to new medium and do some other cultures,,(veggie seeds)...so a drop wouldnt, hurt of this eco or of a o/1//1 hydro nutrient additive...
AND my agar powder says 2 tbls per 1 pint about a half litre right? would you use this amount in your mediums,,,cause my readings say about half that in TC.. your opinion...

Had a thought on a new KIT... ALL is in there. tent,lights,cooker,tots,etc,, just like hydro did when it went public....I was 17 in my first appt. (with a 19 yr.old chick)22 years ago,,put a seed on my fish tank and it sprouted,,the next issue of high time had a article on HYDRO,,kidda like what we're doing right now...but im not just going to think about it this time...objectives/goals=SHOT ASS LIFE!!!

Click to expand...

I way over did it on my first hormone experiment, still suffering the fallout. I still have 3 tables exposed to my "bad" mix in rotation and atleast 1 of them looks like it will almost be a complete loss...

If you want to get technical a pint is 473 milliliters but I think 500ml (1/2 liter) would be close enough for a gelling agent...

If you want to be really accurate just weigh 2 tablespoons of powder and do the math

By volume package instructions give you what looks like 1cc per 15.76ml or 1 teaspoon to every 78.83ml
Where as 4 tablespoons per liter (or 2 tablespoons per 500ml) would give you more like 1cc per 16.66ml (or 60cc's per liter)
If you have a 1/3 teaspoon measure and use it in conjunction with your 2 tablespoons it would (more or less) account for the extra volume (water) in a half liter. Hell 1/4 teaspoons are fairly common and will get you closer to the label concentrations than adding nothing extra at all (just make it a "heaping" 1/4 teaspoon).

Did you look into the "SuperNova" or "Nitrozyme" products the guy in the video mentioned? Atleast one of them is a seaweed extract (whatever that means anymore, I know that is used as a base to some "miracle" additives, that they manage to sneak other "proprietary" stuff into).

that chinese paper lays it all out specifically whith correct ratios for desired affects. ppm, sugar,eco salts,water are the same for me in every culture. iba naa and bap are in every culture vessel I have, just at varying amounts, two drops of this to root, with one drop of that, and this many to divide, etc. no sense on me reinventing the wheel here, scientists have already done it. I never use gibberellins. eco is mixed according to directions to attain a under 200ppm mix and works here consistantly, with no other nutrients, besides sugar. I dont even care why it works, now that I know it does !! The public carrot callus recipe is successful for callus production and division also. a necessary starting point for tissue culture.
Three stages, three different vessels, only three hormones, used in conjunction, at varying rates, depending upon desired effects. the difference between my callus and rooting gel is two drops more of one hormone.

I'll be honest, I built every system I ever had in 20 yrs or so, and used off the shelf stuff constantly. but when i got legal all of that stuff went into the barn, and I wrote a check for pro systems, equipment, and supplies that was paid for in a short time, and have nevr looked back. It was fun, even getting wet, or stinky, or having strange results.....but not fiscally responsible. now I get no surprises ever,no leaks,no experiments or additives, and consistant results...which I never got from home depot supplies. cant say i regret either way, but should have wrote a check yrs ago,instead of trying to reinvent this damn wheel. from lighting to nutrients to c02 to temps and humidity, I started to feel like a retired old dude farting around tinkering, now I feel like a retired old dude farting around and tinkering, but getting consistant results and cashing in from them.

peace


Callus Production/division

Rooting


Shooting

hardening off

pharmacoping said:

that chinese paper lays it all out specifically whith correct ratios for desired affects. ppm, sugar,eco salts,water are the same for me in every culture. iba naa and bap are in every culture vessel I have, just at varying amounts, two drops of this to root, with one drop of that, and this many to divide, etc. no sense on me reinventing the wheel here, scientists have already done it. I never use gibberellins. eco is mixed according to directions to attain a under 200ppm mix and works here consistantly, with no other nutrients, besides sugar. I dont even care why it works, now that I know it does !! The public carrot callus recipe is successful for callus production and division also. a necessary starting point for tissue culture.
Three stages, three different vessels, only three hormones, used in conjunction, at varying rates, depending upon desired effects. the difference between my callus and rooting gel is two drops more of one hormone.

I'll be honest, I built every system I ever had in 20 yrs or so, and used off the shelf stuff constantly. but when i got legal all of that stuff went into the barn, and I wrote a check for pro systems, equipment, and supplies that was paid for in a short time, and have nevr looked back. It was fun, even getting wet, or stinky, or having strange results.....but not fiscally responsible. now I get no surprises ever,no leaks,no experiments or additives, and consistant results...which I never got from home depot supplies. cant say i regret either way, but should have wrote a check yrs ago,instead of trying to reinvent this damn wheel. from lighting to nutrients to c02 to temps and humidity, I started to feel like a retired old dude farting around tinkering, now I feel like a retired old dude farting around and tinkering, but getting consistant results and cashing in from them.

peace


Callus Production/division

Rooting


Shooting

hardening off

Click to expand...

Which was the "Chinese" one? What plant variety/s did they base their information on (Isn't pot one of those things that gets you killed in China). I have read things about tropical fruit that just doesn't seem like it would yield the same results in marijuana..
I have seen similar experiments conducted: one with cannabis the other an ornamental flower, cereal crop, vegetable or fruiting plant and the hormone concentrations/ratios were completely different...

I can relate with what you are saying to some extent... Since I became legal I have done 2 complete grow room redesigns using all the hydro shop stuff (once for just me, and again when I picked up a partner, to take advantage of "shared growing space"). To be honest with you I have been disappointed many times with the limitations of the hydro store stuff...
My Batinacare 3' tables are more like 42 inches and don't properly fit in the only 3 foot stands I could find (clearly meant for like 38") .. A 6.5' grow tent I bought was more like 6'2" or 6'4" (don't remember now because its been given away.. Hydro shop pumps are expensive for what they are, $150-200.oo reflectors that don't distribute light any better than home made ones...
My grow room looks impressive to everyone who has seen it (been offered store credit if I slap a bumper sticker on my system and take a picture of it for advertisement), yet it rarely fails to piss me off more than most the setups I built from scratch... There are some hydro store parts I have evicted from my garden... The overflows on my flood tables for instance got replaced with pluming parts from Lows because the hydro shop stuff has no adjustment finer than about 1.5-2" But nobody can see that so I still look high-tech.... Not to mention finding flood table covers with any spacing other than 25 plants per square meter (obviously I still make my own)..

To be honest I'm pretty well fed up with the entire hydro shop industry... Literally nothing it has to offer lives up to the expectations they imply, so you will buy more crap to reach your otherwise failed expectations, then they sell you something else so you can be as efficient as you should have been considering your previous upgrades. Its rediculis, and the impressions they place people under... Have you looked around here and seen what some people are spending on nutrients (not even fully knowing whats in them). I read an article recently about Dutch Masters Superbud... I'm sure its old news now but it ilistrates my point perfectly..

I deal with greenhouse supply companies now, they will tell me whats in almost anything I buy (so there are no mysteries) and everything is sold in bulk making it convenient and cost efficient. And most the freight truck drivers have no issues putting things where you want them... Beats the hell out of struggling with 5-6 gallon jugs in/out of the shop, your car, and your house. When I built my latest grow room I made sure it had an outside door and small porch (makeshift loading dock) to make deliveries easier (room construction would have been a nightmare without it).

http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf
thanks to nitro ! for this one. It's almost the same ratios I use in vitro. i wish he showed this to me twenty yrs ago !!