My Clone From Leaf Experiment

jriggs nice avatar "watershipdown" i havent seen that for 20 years brings back memories of childhood i use to watch that all the time. interesting work with those sprouts

How long does this process take?? I still don't think its any easier than pruning
a branch and tossing it in the cloner.

Budsworth said:

How long does this process take?? I still don't think its any easier than pruning
a branch and tossing it in the cloner.

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well, it took me a year to finally come up with this working protocol, now that i've figured all that stuff out it would take roughly 2 or 3 months to go from seed to rooted clone.

Still a lot easier to have a good mother plant and take clones from her.

I've never done a tissue culture (never saw the reason to) but this website has lots of info on the subject:

Kitchen Culture Kits - Intro Page

It seems like a pain in the butt compared to clones.

hey tmb77 thanks for the quick tutorial.I have had many leaves take root accidentally over the years.This prompted me to try quite a few different ways to clone them.I have to say in all the rooted leaves I've seen none have produced a plant. Previous to the culture post I was wondering if maybe the selected test leaves were already in the dieing phase of a leafs life.Your experience with the culture method makes me think this even more.Maybe the newest smallest leaflets should be used. Or perhaps forcing a flowering plant back into veg and using the first single blade leaves.Fun to think about these things , but overall I concur with most that unless you are going to tissue culture 1000's in the lab it's probably not worthwhile.

well ouizer, I really believe that people are not far off. if you can form roots you're halfway there...all you need to do after the roots have formed is supply a different hormone ratio to encourage fast shoot formation.

basically when the roots are forming you have a high Auxin:cytokinin ratio, if you can switch it to a high cytokinin:auxin ratio shoots should form. I'm currently trying to find cheap sources of cytokinins instead of buying the stuff my lab usually uses...cuz it's mad expensive.

I've not forgotten about all this...I'm hoping to start a new thread (including....PICTURES!) in the next few months where I actually make this happy.

Nitrozyme is a product that contains cytokinins.


Nitrozyme - Organic Fertilizer, Hydroponics, Diatomaceous Earth, Hydrogen Peroxide, Agricultural Fertilizers, Gardening Products

techhead420 said:

Nitrozyme is a product that contains cytokinins.


Nitrozyme - Organic Fertilizer, Hydroponics, Diatomaceous Earth, Hydrogen Peroxide, Agricultural Fertilizers, Gardening Products

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ah, nice find man...I might have to check that out.

although, it will have to wait till I feel like spending 30 bucks or so on an experiment for this crowd, I really dont have much use for the stuff myself. I was going to crack some old texts tonight to see if there is a local plant I can grab and process in some way to get the hormones I need, but it's nice to know I could just order some if i wanted. (kicking myself once again that I didnt grab some hormones from the lab before leaving for my new job)

tmb77 please help me you know your stuff so i figured id ask
ive been looking up micropropagation and every time i try to create sterile conditions the water ends up slightly yellow and the leaves die when i open up the test tubes they kind of smell bad
ive done dozens of cultures and kind of have limited materials(no access to rooting hormones or agar) but have done my best to provide nutrients for the cuttings

im open to anything please help

Guerilla Grower said:

tmb77 please help me you know your stuff so i figured id ask
ive been looking up micropropagation and every time i try to create sterile conditions the water ends up slightly yellow and the leaves die when i open up the test tubes they kind of smell bad
ive done dozens of cultures and kind of have limited materials(no access to rooting hormones or agar) but have done my best to provide nutrients for the cuttings

im open to anything please help

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I need a lot more details than that, what exactly are you trying to do?

I gather you're using test tubes filled with water to try and propagate herb?

where are you getting the water from...the tap?

what nutrients?

what sterilization method?

what tissue from the plant, at what age of growth?

Another source of cytokinins is coconut milk. You can find some recipes for tissue culture on google.

coconut milk tissue culture - Google Search

these are good links you're supplying

my one concern with methods such as this is the unknown amount of the hormones in them. I cant seem to find any amounts (as in mg/l) or any molar concentrations. when i've done this before it was with known concentrations, so I was able to supply exactly 2mg/l cytokinins to 0.1mg/l auxin, the magical shoot regeneration formula. I suppose at worst it would take some experimenting with amounts to figure out what is needed.

anyways, good research tech, you planning on trying this yourself?

good work impressive

"anyways, good research tech, you planning on trying this yourself?"

Nah, I was going to get into years back and I went out and bought a bunch of books on the subject but the more I understood the more I realized that, for pot at least, it just wasn't worth the hassle considering how easy cloning is. Experimenting with cytokinins, auxins and gibberellins is interesting though.

I do, however, encourage people to try tissue culture; it could be a fun way to expand a hobby.

MsMILFweed said:

Is that you who has?? What happened after it rooted.. nothing. You just had a rooted leaf?

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that is some Dank ass bud!
LOL

TMB77:

What's your opinion on the methods from the original post? Do you think it's possible to propogate with the techniques that Tet and Milf tried? Or was Woo full of shit?


I just spent an hour reading this thread

It was incomplete and overly optimistic



https://www.rollitup.org/advanced-marijuana-cultivation/88059-fanleaf-clone-protocol-homegrown-science.html

ya know i have been watching this post and all i can say is in the time we have spend with it we could have harvested about a metric ton of sticky each from conventional cloning.

if it aint broke dont fix it.

by the way i am still waiting for the op to show us one example of this actually working as he claims he does constantly.

i suspect he is blowing smoke up our asses and finding all of this amusing.

i just stumbled on something that may clear up some stuff

Super Starts Plant Tissue Culture - Home
it seems that a company makes a tissue culture kit. it aint cheap
the trick is to figure out what it is and reproduce it and bypass the 250 pricetag

mrbuzzsaw said:

i just stumbled on something that may clear up some stuff

Super Starts Plant Tissue Culture - Home
it seems that a company makes a tissue culture kit. it aint cheap
the trick is to figure out what it is and reproduce it and bypass the 250 pricetag

Click to expand...

Includes:
30 Baby food Jars and PP tray
60 Tissue Culture plastic jars lids

4 Packs MS Media Mix - *soluble powdered nutrients*
4 Packs Gelling Agent - *agar, 'agar agar' can be found in many health stores.
4ml BA Shoot Multiplying Hormone - *cytokinins*
4 ml NAA Root Inducing Hormone - *Auxin*
4 ml PPM Preservative Mixture - *Probably just thiamine, but they might have stuck some other stuff in there*

Stainless Steel Forceps and Scalpel
Sealing Tape, Droppers and Measuring Spoons
pH Testing and Correction Kit
DVD or VHS video 50-page Illustrated Instructions with Recipes




hope any of that helped.