Using Gibberellic acid on old Seeds?

Discussion in 'Breeders Paradise' started by Gbuddy, Jan 4, 2018.

  1.  
    vostok

    vostok Well-Known Member

    The measure of a man is not the make of car or the length of his dick

    but the way he would make a deal

    you explain to some one better than you that you have an issue that he may resolve

    and for that assistance you would part with say 25% of the goods

    eventually he'd come down to 33% in which case you 'would agree

    after all you have to ask where all these 100's of breeders get their seeds from

    its all in the attitude you adopt to create that deal

    good luck
     
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  2.  
    Gbuddy

    Gbuddy Well-Known Member

    I was searching for home use tissue culture.
    And damn fucking steril everything got to be.
    :-) I have no Lab. Sad but true.
    And I am hard working tax paying man with lil of time for hanging around university doors to find a student to do the Job.
    But its the best idea to do so because I am sure I will mess it up at home. Ask my Doberman about sterility :-D

    Once apon a time...
    I grew shrooms and started from spores all the way down to petri dishes to corn cultures aso. All steril jobs involved were hard to accomplish for me at home.
    My first inoculation box I blew up because I sterilized the box and then put the burner in to early and BOOOOM !!
    Burned all hairs off of my hands lol
    But believe it or not after all I ended up with a lot of them nice lil ni┼łos santos.

    They not even crack the shell

    I did a run using a solution of hydrogen peroxide solution @~ 1-2% in papertowels last week.
    DIDNT WORK for the old beans.
    Had a side by side experiment with my old seeds and fresh seeds.
    Fresh ones sprouted old ones not.

    I ordered my ga3 today.
    If it dosnt work I ll try B1.

    I wont give up till the last seed is gone!
    That was the method I used first.
    Didnt work for this seeds.
    I am in a Land of mj prohibition.
    The guy at the nursery would just call the police when I would sail around his place with cannabis seeds to germ for him :-D

    I ll keep you updated
    Hope the ga3 arrives by next week.

    Peace
     
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  3.  
    Gbuddy

    Gbuddy Well-Known Member

    Not that easy in a Land of mj prohibition.

    I have sent 100's of seeds (from the same storage box but other strains) over the big pond down to some skilled american friends.
    Not one seed germinated successful.

    Its the way they were stored.
    But its somehow strange because those 3000yr old cannabis seeds the russians found in a thomb they germinated successful. No fridge in the thomb... just an airtight sealed container like mine
    Ok they have labs and scientists and a couple.of thousa d seeds.....

    Thats why I refuse to give up.
    It can be done for sure...
    But what are the expences?

    My money and time is limited.
    If prohibition wouldnt be here I would go to the next university and talk to the right people...
    But untill that I am on my own legs.
    That sucks.

    Peace

    Edit: I maybe try to put some seeds in the fridge for some time. Maybe a simulated winter can reactivate someting.
    But first of all I ll try ga3 next
     
    Last edited: Jan 14, 2018
    vostok likes this.
  4.  
    ANC

    ANC Well-Known Member

    Practice growing shrooms from a spore print once or twice. You should master most of the sterile practices at home level doing that.
     
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  5.  
    Gbuddy

    Gbuddy Well-Known Member

    Dunno bro..... its a f***** pain in the ass. Hope ga3 will work.
    Next step is tissue culture I know.

    Could do the sterile inocculation over my gasburner could work maybe. As long as its not to complicated to clean the plantmatter. I need more input on that upfront.

    But atm I am a bit frustrated... downloaded a book about tlo.... wanted to brew some foamy teas like the guy but...

    Check this out
    https://www.rollitup.org/t/no-tlo-ingredients-available-in-my-country-f-that.956964/
     
  6.  
    Gbuddy

    Gbuddy Well-Known Member

    Did I mention that I hate my government?... and they hate me back!
     
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  7.  
    ANC

    ANC Well-Known Member

    You make a simple sterile box with dishwashing gloves, a tote, and a box cutter (also make a little see-through window. Sterilize the crap out of it with alcohol.
    Find a small tiled bathroom, make sure all windows are closed for a while so dust can settle. Spray an air sterilizer from a can, and get to work. Plenty of youtube vids to show you the ropes, like never moving your hand over, or reaching over an open container etc.
     
    Gbuddy likes this.
  8.  
    Gbuddy

    Gbuddy Well-Known Member

    If y
    If you know somebody who can do it
    ...I'll share the genetics with you and the people who can get em goin. Just want a copy or two of each pheno myself to keep the stuff around.

    I always try to share what I have. ( and I have more than those old beans.) But they are very special.
    I can send it where ever you want no problem.
     
    Last edited: Jan 14, 2018
    farmerfischer likes this.
  9.  
    Gbuddy

    Gbuddy Well-Known Member

    Building a inocculation box is not the problem bro....
    I know how to make one nicely after frieing my hands.....

    The kit I found that looked nicely was 176$
    And the diy version is even more expensive and also complicated lol.
    So its the last thing I'll try.
    They dont pay me good at work.
    A poor fella like me always checks a dime two times if you know what.I mean.

    The Plan is ga3 next
    Maybe b1 if ga3 sucks
    Or tissue culture ( I think the kits are better for me)
    But I will have to make a decission.
    Is it worth 200buck and messing it up?
    Dunno need some meditation to make the decission I guess :-D
     
  10.  
    xtsho

    xtsho Well-Known Member

    I've played around with Ga3 in the past for old seeds but not cannabis. I had some twenty year old flower seeds that came from Ft Vancouver and had been in small envelopes in a drawer. I ended up with about a 20% germination rate. I used a 200 ppm solution.
     
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  11.  
    Gbuddy

    Gbuddy Well-Known Member

    Sounds good to me!
    I would be happy ending up with 20% germination rate.
    At the moment I am at zero % ....So 20% would be great! :-D
    I need just a female and a male to get F2 to select from. I know what I am searching so F2 would be what I aim.
    S1 dunno.....wouldnt be good for my purpose....
    There is always some recombination of gen's even in S1 but it would shorten variety big time.... So a male and a female is just ok better would be higher numbers of each males and females to get a better chance to find the phenotype I am loocking for in F2.

    I wont give up... but the Ice is getting thinner with every seed I waste. I fear counting them but its about 15 seeds or so left right now.

    Hope dies last

    Peace
     
  12.  
    Gbuddy

    Gbuddy Well-Known Member

  13.  
    Gquebed

    Gquebed Well-Known Member

    Hmmm....
    I used to collect seeds from the weed I was buying in the 80s before sensimillia come around. 30 years later I started growing and I happen to run across those seeds. They were stored in a baggie in a desk drawer thay sat over top of heat register for a decade.
    Just for shits and giggles I tried germinating some. I got about a 25% success rate. I grew out four plants. They turned out pretty good.

    What point is... if the genetics are strong then the seeds should pop and grow? If not... then the genetics arent so strong and arent worth saving?
     
    Gbuddy likes this.
  14.  
    Gbuddy

    Gbuddy Well-Known Member

    Thats a sugestion...

    Btw. You are a lucky man ...25% ...of fried seeds .... thats a respectable ratio.

    Lets talk about G-netics....
    I know dank strains that will not pop after 2 years of storing, if not stored in the fridge.
    I know industrial hemp strains that will pop even after you cooked them for fishing.
    So any sugestions on that?

    The Strain I am trying to safe here is something special. Most comercial growers would never grow this strain.
    The father is just a sensi seeds skunk#1 male.
    I used it because skunk#1 is not giving much of its traits like taste aso to the offspring in my experience. That was exactly what I needed at this point. And I just had this nice skunk male around at that time.
    I hoped the mother would dominate especially for taste.
    Also hped the skunk will add some yield and better structure.

    The mother I got gifted by a friend named BBQ.
    We shared clones and dope and whatever we had.
    One day he came over with two clones labeled br#6 and br#3.
    The Info he gave me was that it is a black russian from a guy called TGA subcool. After research I only could think of TGAs black russian backcross.

    He grew it and said the yield isnt worth growing it and paying electricity for it. He warned me that they are some dwarfs and so on. But he mentioned some wicked taste. That took my attention.
    I made clones. I kept the moms and put the clones to my other ladies I had after veg.

    Crazy thing was those two plants would finish both after 30 days(BR#6) / 40 days (BR#3)
    But the downside was they ended up less than a foot in size for me and my growbuddy. I did try a lot to trigger them into bigger plants.. pruning.. long veg...etc.
    No Chance ...30-40 days flowernig and 1 foot of height was all I got. After that all hairs were brown and trics ready for harvest.
    30g dry Bud was the best yield per Plant.
    BR#3 was slightly taller, or better a bit more lanky.
    Both 3 and 6 had thin weak stems ang light green leafes. With Popcorn Buds on it.
    6 was darker purple colored, 3 was reddich to light purple Bud from the start.
    They were overloaded with tricomes ...maybe humilated by that coat. Dunno.

    But.....

    The T A S T E
    UNREAL EXTREME EXTRATERRESTIAL ALIENBERRY PURPLE SWEET DEEP FLAVOUR AND TASTE.

    Like some kind of extraterrestrial Alienberries mixed up with some coolaid grape.
    But I am not just talking about a bit of a nuance in the back of smoke... I am talking about a overwhelming extreme kind of taste. Out of this world! Unreal! Supernatural taste. Long lasting taste.

    What she lacked in size she got in taste and flavour.

    I would die to get that taste back!

    I am not a dealer so I dont care too much about yield.

    I think I still have pictures from 6 somewhere on some old harddisk.
    I will search and upload them.


    Peace

    Edit:
    But I have to agree with you that at least the motherplant 6 and her sister were weak plants in most points.
    While the skunk male was a strong sticky motherfucker.
    That still would just make "half weak genetics" at best, right?
    :bigjoint:
     
    Last edited: Jan 19, 2018
    Gquebed likes this.
  15.  
    Gbuddy

    Gbuddy Well-Known Member

    Oops
    Sorry for the wallpaper ^^^
    LOL
     
  16.  
    Gbuddy

    Gbuddy Well-Known Member

    Hi @all

    Found out today that I overlooked that the next good time for Seeds is tomorrow.... was sure it was 1. Feb but I guess ( memo for myself: I should smoke less)

    That means my GA3 on old Seeds experiment will start sooner than I believed.

    Got my stuff ready today.
    Ready to start by tomorrow or Sunday.
    Will take some pics and post the news.

    You guys should send me some karma to help...

    Peace:bigjoint:
     
  17.  
    xtsho

    xtsho Well-Known Member

    Good luck!
    Got my fingers crossed hoping it's successful.
    You can't send karma it's earned. You seem to be on the right path. So no worries for you.
     
    Gbuddy likes this.
  18.  
    Gbuddy

    Gbuddy Well-Known Member

    Thank you very much!!
     
  19.  
    Gbuddy

    Gbuddy Well-Known Member

    Ok here we go.....

    Here some pics of the materials used for the experiment.
    20180127_155026.jpg 20180127_155205.jpg 20180127_155418-1.jpg

    Not on the pictures is the destilled water I used.
    The glas jars I bought on last thursday .... have 4...looked just fine

    The seeds in the white envelops are vine grape seeds a Concord from South Carolina, a Concord from Jamaica, and some Finger Grapes. They' re also each in seperate lil plastic containers with water @100ppm GA3 to break dormanz.

    In the lil plastic tube are the old BR6 cross Seeds.
    Today I checked and there were 40 left. So the Ice isnt as thin than I believed befor checking.
    I soak 7 Seeds in the 100ppm solution and 8 in the 250-300ppm.
    I have a side by side run with my viable seeds as a reference.
    So I can see if its the Seeds or the Method that fucks up.

    Good news is...everything worked smooth without problems. Material was in fine powder form...disolved well after a lil shake and some time in the water (just a few minutes).

    Good news 2 ... checking 2 hours past droping seeds into the water, aaand 2 seeds in 250ppm and 1 in 100ppm decided to dive to the bottom of the jar.... promising

    Lets see what we will have after 12 hours.

    I will take the seeds together with some of the solution out of the jars after 12 hours and run them another 12 hours in papertowels. Following the method from the youtuber Vader.
    Will use the transparent containers with the white lid from the one picture above.
    Have also 4 of those.

    Edit

    In jar number 4 I have 2 Seeds of Orange Skunk by Expert Seeds in just plain spring water

    Peace:peace::bigjoint:
     
    Last edited: Jan 27, 2018
  20.  
    Gbuddy

    Gbuddy Well-Known Member

    @all
    Watched the video again and found out I missed something.... its not 12 hours soak in the glas jars its 24.
    That means....

    Step 1
    Soak 12h -> check if the Seeds are all sinking down.

    Step 2
    Let them in the Jars for another 12h

    Step 3
    Switch to paper towel Method for another 12h using the solution from the Jar.

    Step 4
    Planting the Seeds in Jiffy's

    Thats the plan

    Btw.....
    All Seeds except one in the 100ppm Solution are down at the bottom of the Jars already.
    If my old eyes are right one in the 250ppm looks like he cracked the shell a lil bit. But not 100% sure.

    Peace out :peace:
     
    Last edited: Jan 28, 2018
    Sour Wreck likes this.

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