STS and naked anthers

Sprayed this female with the standard STS recipe at the beginning of 12/12, and again a week later. 3 weeks later, this is what developed:



The buds are covered with anthers, very short/no filaments, and no sepals. These anthers did produce pollen after another 2 weeks, but nothing compared to what "normal" male structures would have.

Anyone have a clue as to what causes this? @CoB_nUt suggested it might be a seed or clone dependency, and I'm thinking the STS concentration or application might be to blame. Anyone?

(Note that this is a dup post that I mistakently posted in another thread when it should be it's own. Regrets.)

Interesting.

Seems like I remember less of a filament on "male" flowers made from reversed females and there definitely is less pollen, assumed it was due to the reversal process.

The other possibility is, the small filament could be a characteristic of the particular strain you sprayed. Some regular male flowers have less of a filament than others, so it's reasonable to think some reversed females might too.

If you used the same spray / method on a different strain or had a regular flowering male of the strain that you did spray. you could note how the filament forms on them and gather more information to form a hypothesis.

I found a way to get the pollen out of the naked anthers that don't open.
Speedball Silkscreen and shadow box.
The silkscreen has micron sizes small enough so the anther matter doesn't go thru only the pollen.
Wish I had thought of this earlier,would've saved me a couple weeks of harvesting the resilient anthers' pollen.

@graying.geek it'll be a couple weeks before I can test my hypothesis. Waiting for my clones to get big enough to take clones.

graying.geek said:

Sprayed this female with the standard STS recipe at the beginning of 12/12, and again a week later. 3 weeks later, this is what developed:

View attachment 4387679

The buds are covered with anthers, very short/no filaments, and no sepals. These anthers did produce pollen after another 2 weeks, but nothing compared to what "normal" male structures would have.

Anyone have a clue as to what causes this? @CoB_nUt suggested it might be a seed or clone dependency, and I'm thinking the STS concentration or application might be to blame. Anyone?

(Note that this is a dup post that I mistakently posted in another thread when it should be it's own. Regrets.)

Click to expand...

I was under the impression that STS should be sprayed weekly. Is that correct?

Rob Roy said:

Interesting.
<snip>

If you used the same spray / method on a different strain or had a regular flowering male of the strain that you did spray. you could note how the filament forms on them and gather more information to form a hypothesis.

Click to expand...

Yeah, I have used the same formulation back in the spring on an old school skunk female. She did produce normal looking "balls" with sepals, but never did produce pollen. When I peeled back the sepals the enclosed anther had a few immature pollen grains, but no viable pollen that I could discern. It was similar to what @CoB_nUt described, and I might have been able to salvage pollen using his technique, but didn't seem worth it at the time.

:" said:

@CoB_nUt it'll be a couple weeks before I can test my hypothesis.

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I'll be really interested in your results. This is far from being a new technique in producing pollen so I'm surprised no one is jumping in with experiential advice.

: said:

@Capn-Crunch I was under the impression that STS should be sprayed weekly. Is that correct?

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I've seen different methodologies. On the Skunk earlier in the spring, I sprayed 4 days in a row starting at 12/12. On the most recent DP, twice, a week apart. I've seen discussions suggesting that STS suppresses ethylene production for 3-4 days, so spraying every 4 days for 3 applications, makes sense and is what I'll probably try "next" time.

graying.geek said:

Yeah, I have used the same formulation back in the spring on an old school skunk female. She did produce normal looking "balls" with sepals, but never did produce pollen. When I peeled back the sepals the enclosed anther had a few immature pollen grains, but no viable pollen that I could discern. It was similar to what @CoB_nUt described, and I might have been able to salvage pollen using his technique, but didn't seem worth it at the time.



I'll be really interested in your results. This is far from being a new technique in producing pollen so I'm surprised no one is jumping in with experiential advice.



I've seen different methodologies. On the Skunk earlier in the spring, I sprayed 4 days in a row starting at 12/12. On the most recent DP, twice, a week apart. I've seen discussions suggesting that STS suppresses ethylene production for 3-4 days, so spraying every 4 days for 3 applications, makes sense and is what I'll probably try "next" time.

Click to expand...

https://steemit.com/cannabis/@thecleangame/diy-no-hermie-feminized-cannabis-pollen-mixing-and-using-silver-thiosulfate-solution-sts-updated
I used this article as a guide but didn't get any open pods at all, but the plant was loaded with them, so it must have been working?

Capn-Crunch said:

https://steemit.com/cannabis/@thecleangame/diy-no-hermie-feminized-cannabis-pollen-mixing-and-using-silver-thiosulfate-solution-sts-updated
I used this article as a guide but didn't get any open pods at all, but the plant was loaded with them, so it must have been working?

Click to expand...

Something similar has happened to me, except I was using home made "colloidal silver", which I suspect was on the weak side.

I picked the most swelled pods and manually opened them over a white plastic yogurt container top to collect what little pollen there was. Then applied the pollen to the target female by q-tip and got some FEM seeds. Some pods had virtually no pollen, but there was enough overall to pollinate. Other times I've gotten more pollen using purchased colloidal silver, but never the amount of pollen you typically get from a regular male. Most of the plants that got sprayed/reversed looked pretty ugly by the time they developed male pollen too.

I'll be trying some silver thiosulfate this fall and intend to spray some on the every 3-4 day schedule suggested by graying.geek above.

Rob Roy said:

Something similar has happened to me, except I was using home made "colloidal silver", which I suspect was on the weak side.

I picked the most swelled pods and manually opened them over a white plastic yogurt container top to collect what little pollen there was. Then applied the pollen to the target female by q-tip and got some FEM seeds. Some pods had virtually no pollen, but there was enough overall to pollinate. Other times I've gotten more pollen using purchased colloidal silver, but never the amount of pollen you typically get from a regular male. Most of the plants that got sprayed/reversed looked pretty ugly by the time they developed male pollen too.

I'll be trying some silver thiosulfate this fall and intend to spray some on the every 3-4 day schedule suggested by graying.geek above.

Click to expand...

how did it work out for you? as much as I liked using colloidal silver, the process for me to make it has been an absolute pain in the ass. It takes almost a week for me, the wires are constantly breaking and coming off the silver rods, the rods dont stay in place well when you are taking the lid off all the time for days to clean the rods, I'm sure its all my methods and errors, but It is frustrating nonetheless. I think a big part is need to start a week or two before flower, and spray into flower as well. ive had great success with that method. thanks for any input.

reallybigjesusfreak said:

how did it work out for you? as much as I liked using colloidal silver, the process for me to make it has been an absolute pain in the ass. It takes almost a week for me, the wires are constantly breaking and coming off the silver rods, the rods dont stay in place well when you are taking the lid off all the time for days to clean the rods, I'm sure its all my methods and errors, but It is frustrating nonetheless. I think a big part is need to start a week or two before flower, and spray into flower as well. ive had great success with that method. thanks for any input.

Click to expand...

The silver thiosulfate I mixed "almost" worked. I ran into a space crunch and had to put the girls I began with spraying indoors into a shed at night and outside during the day. I'm thinking that was a mistake and I should have kept them inside in a more temperature controlled environment. Fall cold ended the project. I also suspect the mix I made was off, (likely my error) although the plants had begun making male flowers, none were far enough along to contain pollen. I had 3 targeted strains and none made viable pollen.

I agree, home made colloidal silver is a pain in the butt to make. Have had greater success with store bought. Have gotten fem seeds both ways though.

I'm in the midst of making clones to try again. Still have the material to make silver thiosulfate and have a bottle of 3 year old store bought colloidal silver. The colloidal silver has been stored in the dark and wrapped up, not sure, if I'll use that or try to make a better batch of silver thiosulfate.

I suppose "in the interest of science", I could do a side by side by side and use the older but unopened colloidal silver, silver thiosulfate and newly purchased colloidal silver on 3 different clones from the same mom..hmm.

Rob Roy said:

The silver thiosulfate I mixed "almost" worked. I ran into a space crunch and had to put the girls I began with spraying indoors into a shed at night and outside during the day. I'm thinking that was a mistake and I should have kept them inside in a more temperature controlled environment. Fall cold ended the project. I also suspect the mix I made was off, (likely my error) although the plants had begun making male flowers, none were far enough along to contain pollen. I had 3 targeted strains and none made viable pollen.

I agree, home made colloidal silver is a pain in the butt to make. Have had greater success with store bought. Have gotten fem seeds both ways though.

I'm in the midst of making clones to try again. Still have the material to make silver thiosulfate and have a bottle of 3 year old store bought colloidal silver. The colloidal silver has been stored in the dark and wrapped up, not sure, if I'll use that or try to make a better batch of silver thiosulfate.

I suppose "in the interest of science", I could do a side by side by side and use the older but unopened colloidal silver, silver thiosulfate and newly purchased colloidal silver on 3 different clones from the same mom..hmm.

Click to expand...

thanks for the input. I think i'm gonna go ahead and order the stuff to make it, and give it a shot. for scinece!

reallybigjesusfreak said:

thanks for the input. I think i'm gonna go ahead and order the stuff to make it, and give it a shot. for scinece!

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I don't think you can go wrong buying the reagents. It's a lot easier and you have known strengths.