Return of the Spork
Well-Known Member
How do you keep stem strength when cloning? I've tried Al's method and I have a bubble cloner going right now.
The bubble cloner is showing more success than the RW did, however most of them are drooped over and some won't recover from that part alone.
Is there a technique when cloning to strengthen the stems before or while cloning? I have a fan on the mothers and the stems seem very sturdy before cutting, but then they just turn into wet spaghetti regardless of thickness.
Any ideas? Temp and ventilation don't seem to be the problem, I cut sterile as I could, only thing I can think of is that there is a step missing somewhere in the cutting process. Ideas?
The bubble cloner is showing more success than the RW did, however most of them are drooped over and some won't recover from that part alone.
Is there a technique when cloning to strengthen the stems before or while cloning? I have a fan on the mothers and the stems seem very sturdy before cutting, but then they just turn into wet spaghetti regardless of thickness.
Any ideas? Temp and ventilation don't seem to be the problem, I cut sterile as I could, only thing I can think of is that there is a step missing somewhere in the cutting process. Ideas?