Skywalker OG tissue culture journal

I just started tissue culturing, and would love to share. What I hope to accomplish is to get RID OF MY MOM ROOM! So this is where it starts! I bought "super starts tissue culture kit and so far so good.

I took 30 planlets on sept 9 2011. Here are pics and my clean room

Here they are 5 days later. I have lost 3 to mold.

Here arethe three moldy ones.

Here they are 7 days in culture. I have a callus that I took from a clone in there too.

These are 12 days in culture. Tonight I will take more planlets and put some of the better cultures together.

Here are two of them around 13 or 14 days in culture.View attachment 1801012

4 years and 420 posts. Whoot whoot!

so what exactly are you trying to do here? i've never heard of this before

either have i but it kinda seems like it works

hazeynights said:

so what exactly are you trying to do here? i've never heard of this before

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trying to turn this.

Into something that resembles this. Lots less space, lights, bugs, deterioration of genes, AND MAINTENANCE!!!! there are lots of benefits to being in culture. Besides being able to propagate on a huge level with less light and space but you can Regenerate plants that have had gene damage over the years(clone only strains). You can also breed and mutate genes. If your smart enough you can extract DNA and isolate genes. Put those genes into agrobacterium and transfer them into other plants. I have heard of people isolating the gene that's responsible for THC production and put it into tomatoes, cucumber, and orange trees.

so sick. subbed! rize up!

Okay - this is definitely a step above! I dig your mind, man.

budforever442200 said:

Here are two of them around 13 or 14 days in culture.View attachment 1801012

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Oh wow, they're growing a ball at the bottom! The last time I looked in this thread, I wasn't sure what was going on with them! Are they working towards growing a root system?

ChairmanMaow said:

Oh wow, they're growing a ball at the bottom! The last time I looked in this thread, I wasn't sure what was going on with them! Are they working towards growing a root system?

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Not right now. They are in hormones for JUST shoot growth, so they will grow with no roots until I put them in a rooting based media. Then they will grow roots. I am making a video on how to do it, it's hard to film, but once I have all the steps I'll post it here.

im in for this ride, got a question??? ive got a pheno thats outside in full flower, it will be lost if i cant reveg or tc it. will tc work in this situation?

hit us up with links to your research sites so we can get the tech side of things without you working your ass of lol also where can i buy the kit your using. im really interested in learning this technque n understanding its capabilities.

4tatude said:

hit us up with links to your research sites so we can get the tech side of things without you working your ass of lol also where can i buy the kit your using. im really interested in learning this technque n understanding its capabilities.

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Well there is tons of info, and I'll try my best to post it. I will post all links on my fist post. Good to start here http://en.m.wikipedia.org/wiki/Plant_tissue_culture
If you google "super starts tissue culture kit" that is what I started with. It has everything you need to begin. And o.k. Directions.
I will post some other useful links later. There is also a tread by Canndo that is very good.

I picked up a tissue culture kit up at my hydro store about 2 months ago. Still haven't had the time to open it. How has your experience been so far?

suTraGrow said:

I picked up a tissue culture kit up at my hydro store about 2 months ago. Still haven't had the time to open it. How has your experience been so far?

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Really good. You just have to be clean, very clean.....BUT IT'S FUN TO TAKE CUTTINGS AGAIN