Yes, I do it all the time. I like to do it just before the night cycle so that I can move the plant away from the rest. Cover your plant with a big plastic bag leaving one of your lower branches exposed.. Dust it with pollen (I use a small artists brush dipped in pollen) Cover it up with a paper towel or piece of cloth completely and remove the plastic bag. Let it sit over the dark cycle away from the rest of your grow if you have more plants. Replace the big bag over the plant in the morning and remove the bud covering and mist it thoroughly with water to kill the loose pollen so any stray pollen doesn't pollinate your other buds. In about 6-8 days you should seeds develop. It takes at lest 5 weeks to mature seeds so pollinate early so you can harvest everything at once.