Purchased a Dissolved Oxygen (DO) meter

squarepush3r

Well-Known Member
I posted this in another forum, but should find it interesting here also.

So I got a DO meter, to run tests and experiments on how to make the water oxygenated. This applies for aeroponics, bubbleponics, DWC, and most hydro systems. The goal is to get a high amount of oxygen in the water, between 15-30 (super saturation) mg/l score of DO for optimal growth. Here is a normal saturation table, which shows normal saturation values.
http://www.aquatext.com/tables/oxygen.htm

For temperatures of about 70 F, DO saturation is about 9
My first test was to take an empty, clean 18 gallon Tote, and fill it with tap water. Did so, and measured the DO. It showed about 7.1. Then, I inserted an airstone similar to a DWC system, and ran it for several minutes. The results stayed nearly the same, maybe increasing to 7.3 possibly. Next test, I inserted a needle wheel pump into the tote, and ran it for several minutes. Same results as the air stone.

Finally, I ran a test bubbling Ozone (O3) into the tote from a 600mg Ozone Generator. Same results.

So far, all 3 methods applied to the water supply, had little to no effect on increasing the DO levels. I was a bit surprised here.


For my final test, I decided to try this out in a 'real world' situation, into an operating DWC system with plants and nutrients. My current system lacks a chiller hooked up, which will be fixed soon. However, water temps were very high, estimated 80 F. I tested the water while the air stone was running. It showed approximated 7.4, still within a close range of our other 3 tests. Now, I decided to turn the air stone/air pump off, wait a few hours, and see what happens (ie: basically running a DWC system without an airstone, just plan water tank). I came back several hours later, and the results were shocking. With the airstone off for nearly 3 hours, the DO levels showed nearly MAXXED out my meter at 18-20 gm/lt. (my meter only reads from 0-20). So, with the air stone off for several hours, in a live plant environment with nutrients, the oxygen took on nearly double the oxygen saturation rate.


From my hypothesis, the plant roots would 'eat up' the oxygen and the DO levels would be lower after my wait. It turned out the opposite to be true. Now, I will say, I just got my meter and I seem to be operating it correctly, but the calibration might be off, or I may be doing it wrong ... but still these results I really can't come up with an answer to explain them.

My DWC container isn't completely light proof, its possible algae may be increasing the oxygen in the water somehow? I read they decrease the oxygen. Maybe the salinity/temperature is throwing the readings off?

Any comments or questions ? I can run some tests with my meter with hypothetical situation
 

repvip

Well-Known Member
At the very least, test your plain tap water again and see if the results are what they should be.

Something seems a little fishy to me... but I don't have a DO meter.

You could test hydrogen peroxide to see what that does on DO ;)

What kind of meter did you get?
 

squarepush3r

Well-Known Member

Cow Tea

Active Member
Well you could always test two dwc buckets: one with a timer, one running constant. Sounds interesting
 

KittyCat

Member
Please run...
1) With H2O2
2) Overnight w/airstone (I'm guessing the bubbles take a while to saturate the water. ie. slow O2 thru water surface tension.)
3) Buy one of those new fangled thingy that measures temps. :)

--------------Kitty
 

squarepush3r

Well-Known Member
3) Buy one of those new fangled thingy that measures temps. :)
will do in 1 and 2 kitty.

but whats 3??? :blsmoke:

Ran the Hydrogen Peroxide test. My cup of water was stable at 6.5. Added the H2O2, and it jumped to 8.5 immediately, then slowly went to about 7.6 in 2 minutes time. Held at 7.6 for a few minutes, and sitting here now still. Ill check it again in an hour or so
 

repvip

Well-Known Member
will do in 1 and 2 kitty.

but whats 3??? :blsmoke:

Ran the Hydrogen Peroxide test. My cup of water was stable at 6.5. Added the H2O2, and it jumped to 8.5 immediately, then slowly went to about 7.6 in 2 minutes time. Held at 7.6 for a few minutes, and sitting here now still. Ill check it again in an hour or so
Hah! Maybe it's a joke for a thermometer?

+rep for doing these tests.. I think it's pretty sweet. And ironic. I don't know why DO would increase without an air stone... what else is going on in that DWC of yours? Is there a recirculating pump or just water with air stones?
 

unity

Well-Known Member
This is pretty cool, I'm going to follow it along. Thanks for running these tests, I'm surprised at the readings as well.

Peace
 

KittyCat

Member
I'm following for sure! Something doesn't make sense, but could very well be something we didn't anticipate. It'll be great to see exactly how much an airstone helps.

I'd like to compare it to an LP aeroponic setup too! I'm running an SB cloner with 10 misters and some thread is saying you gotta have an airstone. ??? I'm blowing water droplets thru the air and that ain't enough?

Jealous of the meter, but I'm more jealous of a 1000 HPS right now. Just a poor stoner. :)

+rep

----------Kitty

3. Thermometer
 

fatman7574

New Member
What are you using for lighting? Are you using lights with an electronic ballast? Is the ballst nera the meter? They cause interference with the meter. Testing conditions should be the same for all prameters except the one thing you are testing ie if your testing for the did feerences with or without an airstone all other conditions such as circulation pumps lighting etc should be the same. In all reality with the grade of the Milwaukee meter I would take water sample in a glass holid the probe and the glass with the same hand and make sure youu keep the water in movement by swirling the glass as the probe must have water moving across the membranee tip. You can't just stick the tip in the water. Either the probe must be kept in contant motion or the water must be in motion. Plus there are two different types of DO meters, one is a Galvanic and the other type is Polarographic. With the Polarographic you must turn the poewer on and leave it on for at lest 20 minutes before you use it to get accurate readings. Every time you turn it off you must turn it back on and agian wait 20 minutes before using it. Kinda sucks but it is a more accuratte reading meter type. Also look at the tips membrane, there should be no wrinkles the solution must be full to overflowing as you put the membrane cap on.

There is nothing in a reservoir that is creating O2. So there is either procedural error electrical interferance or something like the membrane or fli uid problem. Calibrating a DO meter is usually done by calibration in saturated air. Basically it just calibartes my itself in room air. Most DO meters come with a holder for the probes which has space for a sponge or something that can be kept damp. Stored in this tube type holder it is considered in saturated air. So the meter is turned on and it is the Polarograpic type you wait 20 minutes take the probe out of its holder and calibatrate the meter to the probe. then leave it turned on until your done with all your readings.
 

quillo

Member
Also look at the tips membrane, there should be no wrinkles the solution must be full to overflowing as you put the membrane cap on.
True that! There should be no bubbles trapped behind the membrane. It takes practice to put that membrane on without trapping a bubble. I sacrificed many membranes perfecting my own technique.

When water reaches o2 saturation, o2 leaves the water through the air-water interface pretty quickly, so especially in cases where bubble aeration or other kinds of mechanically driven water movement are present, it's hard to supersaturate water to any great extent, at least through biological means. I'm inclined to doubt your water could really be reaching the levels of supersaturation reported by your meter. But then again, what do I know?
 
I wonder what ever happened with this, anyone have any extra to add to this? I plan on doing similar tests once mine arrives with my grow.
 
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