Cellular Response to Hormones in Tissue Culture Tests

How translatable are these results to the real world and established plants? Very few of the experiments I am finding data on seem to extend ex vitro...

Do plants behave differently once they establish roots/are induced to flower?

Or is it just a given that established plants would respond directly in line with the results of the tests? if so would it be at the same concentrations as the tests (for a foliage application anyway)?

On a somewhat related crazy thought.. If you harvest a plant and immediately strip it of all its flower/foliage can you utilize the remaining plant material (nodes/stem) to repeat these tests (assuming you provide the correct environment)? If so you would have essentially cloned that plant... (Yet one more use for the bullet blender... lol, I've never created a test-tube baby).

One of you has got to be doing this stuff at work, school, or in the basement/garage..

I am not sure what you are looking to accomplish but I think most growers are using hormones for cloning. I also know there is a product out there called reverse which is supposed to change sex of a plant which I assume is composed of a plant hormone.

tpsmc said:

I am not sure what you are looking to accomplish but I think most growers are using hormones for cloning. I also know there is a product out there called reverse which is supposed to change sex of a plant which I assume is composed of a plant hormone.

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Thank you for pointing that out. I suppose to some extent I have raised come test-tube babies by way of cloning. Using some of the rooting hormones you mention.. But I was actually thinking about using much smaller pieces to acomplish a similar thing.

Is it "Dutch Master Reverse / Penetrator" you are referring to? The short article I just read sugests that it is more suited to combating hermaphrodisum in plants.

Yes the product is Dutch Masters Reverse. So you're looking to grow a plant from a cellular culture?

tpsmc said:

Yes the product is Dutch Masters Reverse. So you're looking to grow a plant from a cellular culture?

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Think the Dutch Masters Reverse might make a better preventative maintenance measure for females that might be susceptible to stress.

I think I could find that one to be an interesting challenge just a little concerned that it might be outside my means, just getting a feel for how resourceful I might have to be...

How pure/clean does the plant tissue have to be to use in some of these experiments I read about.
Besides until the plants develop roots they are not technically a "pot plant" (at least my my states definition) so it makes experimentation a bit easier..

What are the experiments you were reading about?

tpsmc said:

What are the experiments you were reading about?

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they are mostly dealing with cloning a plant using tissues from the growing tips/node aria (meristematic cells) in conjunction with various hormones to encourage a particular behavior (developing new growing tips or roots) essentially provoking a group of cells to begin producing a plant..

What brought on research was an interest in maximizing the overall performance of established plants predominantly by focusing its growth in secondary growing tips as apposed to the main stem Ideally yielding a plant that is relatively short/squat and dense (particularly in regard to the flowering tips). It would go a long way towards an ultimate goal of successfully cultivating Sativa dominant verity's indoors at a high relative efficiency (make it so you can grow sativa's in a similar manner as you would an indica)

Try bushmaster but be careful a little bit goes a long way, but that is exactly what that product does.

tpsmc said:

Try bushmaster but be careful a little bit goes a long way, but that is exactly what that product does.

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I actually have a quart of the stuff, even tried it but didn't experience the results I had expected. Mind you this was a single foliage application but the lable seemed to caution about over application and not knowing exactly whats in it I felt it wise to just try something else.

Though I may give the bushmaster another try soon as my hopes of finding a good hormone treatment may be dashed by my lack of laboratory grade tools/equiptment..