Beware Spore Farmer

canndo

Well-Known Member
I am not sure this is the place for such information, but I figure I should post anyway. It seems that at least two spore farmer syringes were heavily contaminated, one with bacteria and the other with bacteria and trich. Anyone who does PF will be very dissapointed if they get one of these.
 

canndo

Well-Known Member
2 out of how many?

Two out of three, I believe PESA and Orisa were the two. I have only seen such contamination once before. It is quite obvious that it comes from the syringe - they were squirted on agar and bacteria bloomed whereever the liquid touched the agar. Trich grew on places dead center of the agar - one point, perhaps two in the center MAY indicate asteril practices but considering the needle never got to the center, the air is sterile and the inccubation conditions are reasonable you MIGHT see something growing near the edge of the dish but not in the center.
 

canndo

Well-Known Member
Make that three out of three - Orissa, Pesa, and Mexican. All of them have the same date 1-5-12 looks like. Not sure about this though.
 

spandy

Well-Known Member
Make that three out of three - Orissa, Pesa, and Mexican. All of them have the same date 1-5-12 looks like. Not sure about this though.
Sounds like they had a bad day for sure with 3 different strains all having contams. How have they been in the past, or have you used them before.
 

sonar

Well-Known Member
Good to know thanks for the heads up.

Do you plan on contacting them? I received a bad pan cambo syringe from sporeworks once and they replaced it no problem. Just told them the truth. When I put some solution on my slide and tried viewing the spores under my microscope, identification was very difficult because it contained the spores of other species.


:wink:
 

canndo

Well-Known Member
Sonar, while I do not doubt for a moment that this establishment will replace the bad ones, I will not trouble them, the mechanics don't work for me. It is a pity that the contam was bacteria. If it were another fungus the mushroom could be isolated away. You place a second dish of agar over the first like an agar blanket - then you race the fungus, this keeps them from intertwining, keeps spores from the fungus you don't want from flying off to recolonize and usually, you will find that the mushroom will grow faster than fungus. At a certain point you can see through the agar at pure mycleium, then you slice through the sandwich and lay a slice on a new dish. It works rarely however, in competition with bacteria.
 
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