Auto-flower tissue culture

I am experimenting on putting autoflower seeds into tissue culture. Any advice would be greatly appreciated.
My plan is to sterilize the seeds and place in tubes with 10 ml initiation media. When the seeds grow into seedlings with 3 sets of leaves (5weeks), I'll place them under a microscope and take 4 meristems (undifferentiated stem cells) from each plant, as well as the tip of the taproot. Hopefully, duplicating the meristem multiplication process will work for about 5 rounds, in tissue culture.

With luck I can multiply the callus too, and maybe stumble across a media that produces embryos, or at least initiate shooting and rooting with no flowers. Will submit photos soon. Doing 1,000 feminized Autoflower skittles.

Can you give us your goal in layman’s terms? I think you are working on a whole bunch of clone creation from a few seeds?

anexgrower said:

Can you give us your goal in layman’s terms? I think you are working on a whole bunch of clone creation from a few seeds?

Click to expand...

anexgrower said:

Can you give us your goal in layman’s terms? I think you are working on a whole bunch of clone creation from a few seeds?
[/QUOTE

Click to expand...

Im starting with 1,000 fem autos ... the goal is to multiply them in tissue culture without them flowering in the tubes. Strategy is to only trans

transfer stemm cells. Google can explai
the details and concepts

Phenocloner said:

Im starting with 1,000 fem autos ... the goal is to multiply them in tissue culture without them flowering in the tubes. Strategy is to only trans

Click to expand...

Just stop. We don’t need any frankenweed taking over and enslaving us. Just stop.

All the work is to save strains that treat medical symptoms. So the work is focused, no Frankenweed

Phenocloner said:

All the work is to save strains that treat medical symptoms. So the work is focused, no Frankenweed

Click to expand...

That’s all I needed to hear!

anexgrower said:

Can you give us your goal in layman’s terms? I think you are working on a whole bunch of clone creation from a few seeds?

Click to expand...

cloning using only cells from the stem. potentially thousands of clones using only a few cells.

Eastownclan said:

That’s all I needed to hear!

Click to expand...

Where can I get some of that Frankenweed strain? Lmao

Phenocloner said:

I am experimenting on putting autoflower seeds into tissue culture. Any advice would be greatly appreciated.
My plan is to sterilize the seeds and place in tubes with 10 ml initiation media. When the seeds grow into seedlings with 3 sets of leaves (5weeks), I'll place them under a microscope and take 4 meristems (undifferentiated stem cells) from each plant, as well as the tip of the taproot. Hopefully, duplicating the meristem multiplication process will work for about 5 rounds, in tissue culture.

With luck I can multiply the callus too, and maybe stumble across a media that produces embryos, or at least initiate shooting and rooting with no flowers. Will submit photos soon. Doing 1,000 feminized Autoflower skittles.

Click to expand...

Hello There,
any news on the venture of the tissue culture of the Skittzles?
how's it going with the experiment.

my friends and I are having heated debates about what will come out after the TC sample is taken from a veg aitoflower in week 2 & 3 of the 18hr cycle ,

any information will help this on going stoner debate!
thanks Dr.Drew

...how cool is the name..."autoflower"?....auto...flower. ...autoflower....how friggin sweet is that?...

mudballs said:

...how cool is the name..."autoflower"?....auto...flower. ...autoflower....how friggin sweet is that?...

Click to expand...

ya man,
even the expanded versions of autos renamed as “fast versions”

when u start to cross autos w Regulars!


do u have any tissue culture experience w Autoflowrrs?

TheDirtyDoc said:

ya man,
even the expanded versions of autos renamed as “fast versions”

when u start to cross autos w Regulars!


do u have any tissue culture experience w Autoflowrrs?

Click to expand...

I saw a guy running thru here not long ago that did tissue culture in his kitchen...i shit you not...straight up gangsta clean lab heisenberg stuff.
Hey i crossed an auto with a reg and got the fastest indica known to man...did i make a 'fast version' reg and not know it?..it was in my sig...admins pinched me i guess...im what you call (whispers) an 0utcast...(wink) but yeah they tissue culture in friggin kitchens now dude

auuuttoooo wasshhhhh

TheDirtyDoc said:

ya man,
even the expanded versions of autos renamed as “fast versions”

when u start to cross autos w Regulars!


do u have any tissue culture experience w Autoflowrrs?

Click to expand...

mudballs said:

I saw a guy running thru here not long ago that did tissue culture in his kitchen...i shit you not...straight up gangsta clean lab heisenberg stuff.
Hey i crossed an auto with a reg and got the fastest indica known to man...did i make a 'fast version' reg and not know it?..it was in my sig...admins pinched me i guess...im what you call (whispers) an putcast...(wink) but yeah they tissue cultures in friggin kitchens now dude

Click to expand...

kitchen is the new basement

YES that is how its done!

these ”fast” versions are a beautiful discovery!
funny how some of the greatest things are made on accident sometimes!!

been watching breeders and seed banks for years now create strains for the Medical market and the Dark side. Now tht science has entered the building though federal licensed facilities and RND stuff Sky is the limit now for discoveries!!

keeling the culture intact is the hard part!!

im fast, go speed grower...go speed grower goooo!

TheDirtyDoc said:

these ”fast” versions are a beautiful discovery!
funny how some of the greatest things are made on accident sometimes!!

Click to expand...

ok i was super stoned and happy on a walk but i sincerely appreciate you bringing this to my attention as it seems an exceeedingly enjoyable aspect of growing i would like to explore