Auto Fem Seed Making?

ChocoKush

Well-Known Member
I plan to start Two of the same Autoflower strains at the same time so say roughly they will finish around the same time as well. So on plant One that i use CS method on and hopefully starts making the male parts with pollen quickly will i have enough time to use the pollen on plant Two to make seeds in time before the life cycle is up to harvest? Or would it be best to just to start the plant One first to make the pollen (store in freezer or other methods) and start the Second plant few weeks after i start plant One to have enough time to make the seed?
 

xtsho

Well-Known Member
I plan to start Two of the same Autoflower strains at the same time so say roughly they will finish around the same time as well. So on plant One that i use CS method on and hopefully starts making the male parts with pollen quickly will i have enough time to use the pollen on plant Two to make seeds in time before the life cycle is up to harvest? Or would it be best to just to start the plant One first to make the pollen (store in freezer or other methods) and start the Second plant few weeks after i start plant One to have enough time to make the seed?
I haven't made seeds with auto's but I would probably start the one you are going to reverse a couple weeks before the one you want to pollinate.
 

vostok

Well-Known Member
I plan to start Two of the same Autoflower strains at the same time so say roughly they will finish around the same time as well. So on plant One that i use CS method on and hopefully starts making the male parts with pollen quickly will i have enough time to use the pollen on plant Two to make seeds in time before the life cycle is up to harvest? Or would it be best to just to start the plant One first to make the pollen (store in freezer or other methods) and start the Second plant few weeks after i start plant One to have enough time to make the seed?
Being a newbie I'd just grow out the one plant

2 weeks in start wit the STS or CS on just one lateral/branch

allow to mature take pollen form the branch and apply to flowers on other branches of the same plant

that way you back=cross on just the one strain

good luck
 

OldMedUser

Well-Known Member
From all I've read, and I read a lot about pot, you should use the pollen on a different plant than itself to prevent reinforcing any bad genes tho backcrossing is used for inbred lines.

Using CS is a bit of a crapshoot and can take a while to get the sprayed branch to reverse if it even will. I make my own CS but haven't tried it on plants yet so making my own STS, (silver thiosulfate), as it works faster and better than CS by all accounts.

I have pure sulfur and lab grade sodium hydroxide to make the sodium thiosulfate with but having trouble finding aluminum wire to hold the silver in while carefully lowering it into hot concentrated nitric acid to make the silver nitrate that is part two needed to make the STS.

You should start one plant at least two weeks before the second so you hopefully have pollen ready once the second one is early in flower then pollinate the lower buds unless you want to dust the all the buds to get as many seeds as possible. I've been breeding my own regular seeds for almost 20 years and looking forward to making my own female seeds crossing hi-CBD plants with some of my favorite THC strains.
 

Mgalekgolo

Active Member
I have pure sulfur and lab grade sodium hydroxide to make the sodium thiosulfate with but having trouble finding aluminum wire to hold the silver in while carefully lowering it into hot concentrated nitric acid to make the silver nitrate that is part two needed to make the STS.
Aren't coathangers sometimes aluminum? or aluminum welding wire should work, no?
 

OldMedUser

Well-Known Member
Aren't coathangers sometimes aluminum? or aluminum welding wire should work, no?
Metal coathangers are steel. I used to deliver cases of them to dry cleaners in Calgary when I worked for a chem supply company back in the late 70s. I don't seem to have any welding wire handy as I don't have a MIG welder. Got some 7018 arc rods and a few rolls of solder but they won't work either. ;)

Might check with my neighbour tho as he has a huge shop on his farm with a MIG welder and might have some. Not sure what I can use as an excuse for needing a couple feet of wire tho. He's not into pot and quite religious tho a real nice guy who plows out my driveway every time we get a big dump of snow. Big Versatile tractor with a 14' blade takes him all of 5 min. :)

Just need it to wrap the silver coin in so I can control the reaction as it's lowered into hot nitric acid. Don't want it boiling over onto my hotplate and aluminum doesn't react with nitric acid tho it sure does with sodium hydroxide.
 

ChocoKush

Well-Known Member
Being a newbie I'd just grow out the one plant

2 weeks in start wit the STS or CS on just one lateral/branch

allow to mature take pollen form the branch and apply to flowers on other branches of the same plant

that way you back=cross on just the one strain

good luck
From all I've read, and I read a lot about pot, you should use the pollen on a different plant than itself to prevent reinforcing any bad genes tho backcrossing is used for inbred lines.

Using CS is a bit of a crapshoot and can take a while to get the sprayed branch to reverse if it even will. I make my own CS but haven't tried it on plants yet so making my own STS, (silver thiosulfate), as it works faster and better than CS by all accounts.

I have pure sulfur and lab grade sodium hydroxide to make the sodium thiosulfate with but having trouble finding aluminum wire to hold the silver in while carefully lowering it into hot concentrated nitric acid to make the silver nitrate that is part two needed to make the STS.

You should start one plant at least two weeks before the second so you hopefully have pollen ready once the second one is early in flower then pollinate the lower buds unless you want to dust the all the buds to get as many seeds as possible. I've been breeding my own regular seeds for almost 20 years and looking forward to making my own female seeds crossing hi-CBD plants with some of my favorite THC strains.
I plan to use CS on one whole plant and use the other whole plant to seed it up, idc about a bud harvest from it, might have to try the STS way aswell? is it hard to make the STS?

I'm also going to be doing it all outdoors if that matters any?
 

OldMedUser

Well-Known Member
It's not hard to make the STS if you can find the two parts but I wasn't able to find them here in Canada with Amazon or elsewhere. I would rather not have iffy chemicals shipped up from the states. You need silver nitrate and not much of it and also sodium thiosulfate. Each part is mixed with water to make base solutions then when ready to spray you mix them together to form the STS.

I have the necessary ingredients to make the two parts and a diploma in chemistry to help make sure I do it right so just need to get off my butt and do it tho I'm not ready to flip any plants atm.

You can do it outdoors but you'll want to do it in calm wind conditions so you can make sure to get all the flowers pollinated well. I usually cover most of the plant with a garbage bag leaving one branch out to dust with pollen so the rest of the plant gives me seedless buds. A fully pollinated plant will really cut down on yield and potency as happened by my last grow when I was careless with the pollen. Never drink and pollinate! :D

Do a search here for STS and you should find lots. Here's some info I've gleaned about it all. Most of the links are likely dead but ya never know.

Seems I can't post more than 1000 characters so trying a text file.

Do you make your own CS or plan to buy it? Not sure how much you need but it's not cheap. I make my own for pennies a gallon. Pretty much whatever the power cost me.
 

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OldMedUser

Well-Known Member
It's not hard to make the STS if you can find the two parts but I wasn't able to find them here in Canada with Amazon or elsewhere. I would rather not have iffy chemicals shipped up from the states. You need silver nitrate and not much of it and also sodium thiosulfate. Each part is mixed with water to make base solutions then when ready to spray you mix them together to form the STS.

I have the necessary ingredients to make the two parts and a diploma in chemistry to help make sure I do it right so just need to get off my butt and do it tho I'm not ready to flip any plants atm.

You can do it outdoors but you'll want to do it in calm wind conditions so you can make sure to get all the flowers pollinated well. I usually cover most of the plant with a garbage bag leaving one branch out to dust with pollen so the rest of the plant gives me seedless buds. A fully pollinated plant will really cut down on yield and potency as happened by my last grow when I was careless with the pollen. Never drink and pollinate! :D

Do a search here for STS and you should find lots. Here's some info I've gleaned about it all. Most of the links are likely dead but ya never know.

Seems I can't post more than 1000 characters so trying a text file.

Do you make your own CS or plan to buy it? Not sure how much you need but it's not cheap. I make my own for pennies a gallon. Pretty much whatever the power cost me.
 

Attachments

ChocoKush

Well-Known Member
It's not hard to make the STS if you can find the two parts but I wasn't able to find them here in Canada with Amazon or elsewhere. I would rather not have iffy chemicals shipped up from the states. You need silver nitrate and not much of it and also sodium thiosulfate. Each part is mixed with water to make base solutions then when ready to spray you mix them together to form the STS.

I have the necessary ingredients to make the two parts and a diploma in chemistry to help make sure I do it right so just need to get off my butt and do it tho I'm not ready to flip any plants atm.

You can do it outdoors but you'll want to do it in calm wind conditions so you can make sure to get all the flowers pollinated well. I usually cover most of the plant with a garbage bag leaving one branch out to dust with pollen so the rest of the plant gives me seedless buds. A fully pollinated plant will really cut down on yield and potency as happened by my last grow when I was careless with the pollen. Never drink and pollinate! :D

Do a search here for STS and you should find lots. Here's some info I've gleaned about it all. Most of the links are likely dead but ya never know.

Seems I can't post more than 1000 characters so trying a text file.

Do you make your own CS or plan to buy it? Not sure how much you need but it's not cheap. I make my own for pennies a gallon. Pretty much whatever the power cost me.
making my own CS and it looks pretty simple to make the STS aswell, Do you know how often you need to put the sts on the plants? i read some place and it says like only once? or i may have read that wrong
 

xtsho

Well-Known Member
making my own CS and it looks pretty simple to make the STS aswell, Do you know how often you need to put the sts on the plants? i read some place and it says like only once? or i may have read that wrong

I spray twice with STS. Colloidal Silver requires repeated spraying for ten days to two weeks. It works but I have found it not to be as reliable as STS.

This was a female. This is 2-3 weeks after spraying twice with STS and putting into 12/12.

 

ChocoKush

Well-Known Member
I spray twice with STS. Colloidal Silver requires repeated spraying for ten days to two weeks. It works but I have found it not to be as reliable as STS.

This was a female. This is 2-3 weeks after spraying twice with STS and putting into 12/12.

Nice! Where do you get silver nitrate and sodium thiosulfate from? and is there a special kind of them i needa get or just aslong as there both crystals? as i have seen bottles to buy that are liquid but idk how to percentage it out to do the right dose?
 

whitebb2727

Well-Known Member
I plan to start Two of the same Autoflower strains at the same time so say roughly they will finish around the same time as well. So on plant One that i use CS method on and hopefully starts making the male parts with pollen quickly will i have enough time to use the pollen on plant Two to make seeds in time before the life cycle is up to harvest? Or would it be best to just to start the plant One first to make the pollen (store in freezer or other methods) and start the Second plant few weeks after i start plant One to have enough time to make the seed?
I would start the one you plan to spray about 10 days before the other one.

Sometimes they are stubborn about showing male parts.
 

xtsho

Well-Known Member
Nice! Where do you get silver nitrate and sodium thiosulfate from? and is there a special kind of them i needa get or just aslong as there both crystals? as i have seen bottles to buy that are liquid but idk how to percentage it out to do the right dose?

You can get on Amazon or chemical shops. Good luck.


Sodium Thiosulfate
https://www.amazon.com/gp/product/B00I5R1UU4/ $5.99 113g

https://www.homesciencetools.com/product/sodium-thiosulfate-30-g/ $3.85 30g


Silver Nitrate
https://www.sciencecompany.com/Silver-Nitrate-10g-P6503.aspx $23.50 10g


http://www.rollitup.org/t/colloidal-ionized-silver-solution.982089/#post-14663675

http://www.rollitup.org/t/applying-silver-nitrate-sodium-thiosulfate-mixture-for-fem-pollen.937161/#post-13622861
 

ChocoKush

Well-Known Member
Seems simpler to make the STS and looks to be better results so i guess im try this method out first then! Thanks also you said you sprayed twice how long apart did you do this or both in the same day?
 

xtsho

Well-Known Member
Also do i need to know the ppm or does it not matter just as long as i use distilled water and do the method right?
Just mix by weight and volume.
Also you need to mix it in a certain order.

@OldMedUser posted a text file with exact instructions a few posts up http://www.rollitup.org/t/auto-fem-seed-making.983337/#post-14698034

Make sure not to spill anything as it will stain things and is hard to remove and on some surfaces you can't. I found out the hard way. Now I'm extremely careful when I mix it up and when I spray it.


"Preparation of STS:

First, a stock solution is made. It consists of two parts (A and B) that are initially mixed separately, then blended together. Part A is ALWAYS mixed into part B while stirring rapidly. Use distilled water; tap water may cause precipitates to form.

Wear gloves while mixing and using these chemicals, and mix and use in a properly ventilated area. A mask will prevent the breathing of any dust, which is caustic. STS is colorless and odorless, and poses minimal health risks if used as described here. (See material safety data sheet links below). Note that silver nitrate and STS can cause brown stains upon drying, so spray over newspaper and avoid spilling.

Part A: .5 gram silver nitrate stirred into 500ml distilled water
Part B: 2.5 grams sodium thiosulfate (anhydrous) stirred into 500ml distilled water"
 

ChocoKush

Well-Known Member
Just mix by weight and volume.
Also you need to mix it in a certain order.

@OldMedUser posted a text file with exact instructions a few posts up http://www.rollitup.org/t/auto-fem-seed-making.983337/#post-14698034

Make sure not to spill anything as it will stain things and is hard to remove and on some surfaces you can't. I found out the hard way. Now I'm extremely careful when I mix it up and when I spray it.


"Preparation of STS:

First, a stock solution is made. It consists of two parts (A and B) that are initially mixed separately, then blended together. Part A is ALWAYS mixed into part B while stirring rapidly. Use distilled water; tap water may cause precipitates to form.

Wear gloves while mixing and using these chemicals, and mix and use in a properly ventilated area. A mask will prevent the breathing of any dust, which is caustic. STS is colorless and odorless, and poses minimal health risks if used as described here. (See material safety data sheet links below). Note that silver nitrate and STS can cause brown stains upon drying, so spray over newspaper and avoid spilling.

Part A: .5 gram silver nitrate stirred into 500ml distilled water
Part B: 2.5 grams sodium thiosulfate (anhydrous) stirred into 500ml distilled water"
Alrighty thanks bud! seems to sum up everything i needed to know :)
 
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