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  1. WolfZen

    Cloning Plants By Tissue Culture

    [/FONT][/B] Want kind of rice car do you have by the way?
  2. WolfZen

    Cloning Plants By Tissue Culture

    Would have been a good point except that you aren't altering or manipulating basic cellular DNA by using hormones. Even by layman's terms using hormones is not 'genetic engineering'.
  3. WolfZen

    Cloning Plants By Tissue Culture

    This is a forum, wild claims will probably be questioned, whether for fun or not. That's the way forums are. You could always sell your TC kits legitimately and pay for advertising if you don't want interaction with forum members?
  4. WolfZen

    Cloning Plants By Tissue Culture

    Making a plant glow requires genetic engineering methods, knowledge and equipment. Tissue culturing is not genetic engineering. Why do you keep implying otherwise?
  5. WolfZen

    Cloning Plants By Tissue Culture

    No need to get upset and try to be rude. Why do you keep switching to different accounts?
  6. WolfZen

    Cloning Plants By Tissue Culture

    No worries, can you show some of the results of your super secret processes with micrografting if you don't want to talk about the process itself?
  7. WolfZen

    Cloning Plants By Tissue Culture

    Yeah that's really interesting and all. But let's get back to talking about tissue culturing. You're right, it IS fun and I'm glad you have this thread to discuss it. So can you answer some of my questions?
  8. WolfZen

    Cloning Plants By Tissue Culture

    Ok, electricity usage is top secret if you say so. What protoplast material are you referring to? From the flourescing bacteria you've cultured? How did you extract, isolate, and copy it for use?
  9. WolfZen

    Cloning help please !!!!

    I'd suggest making a bubble cloner. Easiest way I've found to do things and wouldn't cost more than 15-20 dollars from scratch. I don't even bother with domes, spraying, rockwool, etc. Just pop them in and check on them in a week or so and the relative humidity inside here is crazy low.
  10. WolfZen

    Cloning Plants By Tissue Culture

    Be that as it may, could you answer any question directly without getting upset, changing the subject, or otherwise avoiding answering? How are you planning on going from culturing bioluminescing bacteria to having glowing plants (even if it's just for fun)?
  11. WolfZen

    Cloning Plants By Tissue Culture

    Read it and still have questions. Foremost being how are you planning on going from culturing bioluminescing bacteria to having glowing plants (even if it's just for fun)? There's no need to get upset at questions being asked.
  12. WolfZen

    Cloning Plants By Tissue Culture

    How are you going to conduct microprojectile bombardment and/or protoplast electroporation?
  13. WolfZen

    Cloning Plants By Tissue Culture

    Google what information? You won't even state what it is you are trying to do. First it's something about discovering genes and bioluminescing bacteria, then it's nothing to do with genetics. Then what are you talking about? Connect the dots and make some sense by following through with your...
  14. WolfZen

    Cloning Plants By Tissue Culture

    What about plant tissue culture? What does that have to do with bioluminescing bacteria for a start?
  15. WolfZen

    Just wanted to let you know I'm doing good. Definitely get back to me when things are quite.

    Just wanted to let you know I'm doing good. Definitely get back to me when things are quite.
  16. WolfZen

    Cloning Plants By Tissue Culture

    Why are you so upset by any specific questions about what you are trying to do?
  17. WolfZen

    When do you start counting the flowering cycle?

    I would explain it as you saying you harvested your Jack Herer at 50 days after flipping to 12/12. What's the confusion? Edit to add: I agree with SubCool and you by the way. I don't get why more people just don't use the objective 'days post-12/12' convention and put an end to the confusion...
  18. WolfZen

    Cloning Plants By Tissue Culture

    That's the most constructive thing you could do with it. There is nothing else you could do without a lot more knowledge and equipment than you have access to - so why are you asking me 'what else' like it's obvious? You're presenting yourself as someone doing something with genetics, so why not...
  19. WolfZen

    Cloning Plants By Tissue Culture

    What are you going to do once you have cultured some bacteria that can luminesce? That doesn't really get you anywhere except for having some bacteria on an agar plate.
  20. WolfZen

    When do you start counting the flowering cycle?

    Generally, people on the forums here and seed sellers count the days from when they first see active flowering 10-20'ish days after switching lighting to 12/12... and not from when 12/12 lighting was started.
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