Liquid Culture Question

[DarthD3vl]

If I was to make a liquid culture using fresh tissue(from best mushroom), would it be simular to isolating strong mycelium, even though mushroom came from multispore innoculated cake? would my lc be like mushroom clones or still random like multi spore??

is that understandable?

+rep for helping

 

[ANC]

Hi there Ithink I understand your question,
One mushroom can contain fibers from adjacent mushrooms, however you will normaly get lucky and just end up with a cloned isolate, but the only way to guarantee is with agar plates.

I have posted an easy liquid cultre recipy before that you can make with coffee grinds and sugar.

 

[DarthD3vl]

yep that was my question thank you, cant rep you with out spreading more rep around first.....

is that recipie in a thread you posted?

 

[ANC]

yeah, its somewhere on here, might look later, morning rush now.

 

[DarthD3vl]

for sure, im searching for it... still haven't found it though so if you do link me or pm me.


Do you like it better than the light corn syrup and water Lc?

 

[ANC]

Simple tek for Lc's Using filter Coffee

1) You need 1 level tablespoon of spent/used filter coffee
2) 1 Level T-Spoon Sugar,, Best is brown sugar, but white sugar will be fine..
3) 3 Cups (750ml water)

Method
Boil 1 cup of water add the filter coffee and sugar, let it stand for 30 minutes
then filter the brew thru coffee filter or cotton wool.
Add 2 cups of water to the now filtered brew, decant into suitable jars and Pc for 45 minutes.
Allow to cool then inoculate with spores or Agar wedge.

I like it cause I have all the stuff in the house.

 

[DarthD3vl]

Found this in estesj's thread

Easy liquid cultre

1 level tablespoon of spend (used) coffe grounds.
1 level teaspoon of sugar (brown preferably)
1 cup of boiled water...

Mix and let stand for 30 minutes.

Filter through coffee filter to remove particles. Add 2 more cups of boiled water.
Put in jar with some broken glass pieces. jar lid needs two holes sealed with silicon.
when you eventualy take stuff out of the jar you use two syringes, one in each "port".
One syringe is packed with cotton wool, air would pass through this to fill the vacuum as you suck liquid out through the other syringe, keeping airbased contaminants out.


Presure cook for 45 minutes... let cool and and put something in...

And no, you don;t need to buy new stuff... you can make clone cultres as explained above with the LC. or you can take spore prints and make your own syringes... Clones are definately the way to go, as they perform much better, and give nice even flush canopies.

Anything you would like to add?

 

[DarthD3vl]

haha you still beat me lol

 

[DarthD3vl]

Going to start an Lc soon with some mushroom tissue, how long will and lc last not in the fridge?? have one not sure if its still viable... also innoculated some pftek cakes with it then read that thats not ideal... I did 5 pints of wbs also though so hopefully at least it will work for those. this was light corn syrup and water lc, how many cc's of lc is enough for pint of wbs, half pint of brf?

 

[ANC]

I'm not sure, I try and keep it on the short side, the more times the cells divide the waeker the strain gets... a bit like inbreeding.
Slants can be refrigerated up to 2 years. PF tek works realy shit with LC from my experience.
There are fucking billions of spores in a print, use more liquid per print, it doesn't matter if you can see spores or not, they are too small for the eye. even on a faint print I'd make like 2 20ml syringes at least, then try and split about 1ml of that for every 2 jars.

 

[DarthD3vl]

ok I wasn't sure if lc's were like spores syringes I figured they were, I used probably about one cc per jar would have done half that but not very steady hands + dark glove box, well I did best I could. about to pc more wbs jars for grain to grain transfer from the 5 I already started.

do you think that those cakes could be reinnoculated with spore syringe of diff strain, if they show no mycelium growth from the lc innoculations?

 

[DarthD3vl]

You'll see the benefit in the increased gas exchange.

took that from the other thread to continue from,

so i already have ten jars 5 half pint brf and 5 pint wbs that dont have the polyfill, do you think if I put them in my steril glove box and breath them every maybe 3 days it may help? I know it will risk contam but I would only do if they seem to stall.

 

[ANC]

No, just leave em, in fact work out the date 3 weeks from now and don't even look at them again until then, even pinpricks of light cause hormonal disturbance etc...

 

[DarthD3vl]

alrighty, what about if the brf cakes I innoculated with lc dont do any thing can I reinnoculate them with regular spore syringe? i imagine my moisture content would be to off then right?

 

[Sr. Verde]

i repped anc for ya darth


You guys are talking about some cool shit. Making a liquid culture is something that has interested me for a while

 

[DarthD3vl]

haha thanks sr verde,

Another question for you Anc, I read some were you dont soak your wbs over night because it reaches proper moisture in just a few hours, but i read somewere else that soaking overnight is nessacary because there are endospores that cant be killed simply by pressure cooking, so the soaking is also a germination period for these endospores, which after germination or whatever they can be killed by pressure sterilization, so question is do you belive this shorter soak still achieves this or is it simply not nessecary? oh and my last question i think i know the anwser to already but want to check incase

alrighty, what about if the brf cakes I innoculated with lc dont do any thing can I reinnoculate them with regular spore syringe? i imagine my moisture content would be to off then right?

 

[ANC]

I have yet to see WBS catch a contam, every type of grain has a diffirent number of endospores.
It is also exactly the way the ozz cube tek works.

 

[DarthD3vl]

Im using ozz bulk mono tub tek this time, but I still prepared all my grains oldschool... using combination of some wbstek I dug out off of shroomery a few years ago and the grain prep method in the rr and roadkill videos. I've only had like 1 or 2 jars of wbs contam since I started growing shrooms, but I was lead to belive that they were very likely too contam, any ways about to soak some seed for a few hours and pressurecook up 5 more jars tonight to try out your method hopefully with better results.

we'll I think thats all I need for now, thanks i'll update if I get mushies or questions

 

[ANC]

If you sterilise them at the correct temp for the correct amount of time and your jar has adequate gas exchange ports, the only way to get contamed is via your spore syringe.
All and all an overnight soak may be usefull for larger grains, but it tends to germinate alot of birdseed. A simple one hour soak in boiled water to hydrate them drying hulls and then PCing is all the effort needed.

 

[DarthD3vl]

hahaha so baked right now I almost for got to do the polyfill thing gotta run to walmart.....

edit: also funny thing i was gonna send my first question in a pm to you anc, then decided to just make it a thread and low and behold you still answered it, thanks man cant rep ya still i'll rep you again eventually