CSI humboldt thread

bubbahaze

bubbahaze

Well-Known Member
Observe & Report said:
It is clear by now that you know nothing about Phylos and have rejected their results for no better reason than they don't comport with your prior beliefs. You haven't even bothered to look at any of the links I have posted, which would have saved you the embarrassment of posting the above.



He sent in two S1's he made himself to understand how they were related better?? LOL

How would you know if Phylos results suck? You don't because you have done zero investigation of Phylos and their methods and revealed your ignorance of them here for all of us to see.

Phylos clearly can distinguish between a Parent and an S1. It's not surprising because this is decades old technology by now and Phylos is a professional outfit staffed by people with PhDs in molecular evolution.
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Interesting Sir Watson
 
Bodyne

Bodyne

Well-Known Member
are they the ones that say there is no difference between indica and sativa? I do appreciate their hemp cultivation work, though. Phylos Galaxy and beyond! lol
 
Bakersfield

Bakersfield

Well-Known Member
The Chemdogs came from a big bag of weed, so they started life as the result of a herm.
S1's can magnify that potential.
He does test all of his gear in house and seems pretty transparent about the results.
Notice he doesn't sell a Chem D S1?

My Bubba's D is a Chem D S1 x Bubba Kush. It was so unstable during testing that the Nspecta sent them out as freebies, but wouldn't sell them.
They didn't come with a warning label, but he gave me the lowdown.
I got about 4 herm plants out of 10 seedlings.
He's also mentioned that the Black D I just started has a tendency to herm, that's a F1 of Chem D x Mendo Purps.
I'm thinking the D is more unstable not sure on the 3, sister, reunion and however many more they sprouted.
 
Bodyne

Bodyne

Well-Known Member
how odd can it be, that when that ChemD cut was being passed around in Salem Oregon and Portland from 2010 to2015, nobody had herms, the cut was easy to grow and the potency off the charts. Just crazy, ain't it. I grew it and it damned near grew itself. But Im glad to hear bout Nspecta cause if it can happen to him, it can happen to any and everyone. imho
 
Bakersfield

Bakersfield

Well-Known Member
Bodyne said:
how odd can it be, that when that ChemD cut was being passed around in Salem Oregon and Portland from 2010 to2015, nobody had herms, the cut was easy to grow and the potency off the charts. Just crazy, ain't it. I grew it and it damned near grew itself. But Im glad to hear bout Nspecta cause if it can happen to him, it can happen to any and everyone. imho
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He's not cutting any corners and keeps his stable to a minimum, compared to many others with 20 different strains available every 6 months. Seems to work a couple of lines a year.
 
Observe & Report

Observe & Report

Well-Known Member
Bodyne said:
how odd can it be, that when that ChemD cut was being passed around in Salem Oregon and Portland from 2010 to2015, nobody had herms, the cut was easy to grow and the potency off the charts. Just crazy, ain't it. I grew it and it damned near grew itself. But Im glad to hear bout Nspecta cause if it can happen to him, it can happen to any and everyone. imho
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There's nothing odd about it at all. If the alleles that cause the trait are het recessive in the parent then the parent won't show the trait but the offspring sure can. When selfing, each recessive allele has a 25% chance of coming up homo in each seed.
 
Bodyne

Bodyne

Well-Known Member
40AmpstoFreedom said:
Hopefully no one since Chem 91 has been well known to hermi and he even stated on his instagram and I believe on the website as well that I think 30% will herm?
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sure like to bitch in the cannaventure thread bout herms, lmfao. he has a small stable, exactly what is the diff?
 
thenotsoesoteric

thenotsoesoteric

Well-Known Member
40AmpstoFreedom said:
I haven't ever bitched in the cannaventure thread, but yeah a lot of people were I noticed for many pages lately, and it is not like the trainwreck and chem 91 aren't well known for having some bad apples. Don't grow them indoors if you don't have time for your garden and pluckem like males if it gets out control.
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That's what I've read as well. Train wreck and chem 91 are well know for herm tendencies. Been know and talked about on the forums for quite while now.
 
quiescent

quiescent

Well-Known Member
40AmpstoFreedom said:
I haven't ever bitched in the cannaventure thread, but yeah a lot of people were I noticed for many pages lately, and it is not like the trainwreck and chem 91 aren't well known for having some bad apples. Don't grow them indoors if you don't have time for your garden and pluckem like males if it gets out control.
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I think that his pricing via 40% off codes causes some impulse buys where people are not researching before dropping a Benjamin on a pack. I'm guilty of buying tons of seeds from various breeders "because its a good deal" that I regret buying years down the line.
 
J

jayblaze710

Well-Known Member
Observe & Report said:
It is clear by now that you know nothing about Phylos and have rejected their results for no better reason than they don't comport with your prior beliefs. You haven't even bothered to look at any of the links I have posted, which would have saved you the embarrassment of posting the above.



He sent in two S1's he made himself to understand how they were related better?? LOL

How would you know if Phylos results suck? You don't because you have done zero investigation of Phylos and their methods and revealed your ignorance of them here for all of us to see.

Phylos clearly can distinguish between a Parent and an S1. It's not surprising because this is decades old technology by now and Phylos is a professional outfit staffed by people with PhDs in molecular evolution.
Click to expand...
Dude. Here’s the Phylos Chem 91 “relationship” graph. Apparently Chem 91 S1 is just as closely related to Chem 91 as Wifi and Motorbreath. It’s fucking shitty.

I literally spent years researching and conducting these types of analyses. I don’t care if you believe me or not. But from everything I’ve seen, Phylos is pretty garbage. If they want to be treated like a real scientific outfit, they should be publishing in real scientific journals. There are open source journals that would publish it.
 

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Observe & Report

Observe & Report

Well-Known Member
jayblaze710 said:
Dude. Here’s the Phylos Chem 91 “relationship” graph. Apparently Chem 91 S1 is just as closely related to Chem 91 as Wifi and Motorbreath. It’s fucking shitty.
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If you clicked the information button you'd find that Phylos explains this diagram and reveals their methods:

The focal sample is labeled at the center of the image. Any genetically identical samples, or clones, of the focal sample can be viewed by clicking on the center of the image. The next circle shows immediate family members and the outer circle indicates relatives that are on the order of cousins or grandparents.
Family relationships are determined from a metric called identity by descent (IBD) (plink v1.9; Purcell et al. 2007). Specifically, the proportion IBD between two samples is the sum of probability of sharing both alleles at a locus (e.g., AA, AA) and 1/2 of the probability of sharing a single allele at a locus (e.g., AA, Aa). We use a second metric called genetic similarity, to identify putative family members as well as to identify clones. Genetic similarity is simply the number of shared alleles divided by the total number of alleles compared between two samples. Clones are designated as those samples that have extremely high genetic similarity. We set the threshold to be slightly higher than the average technical error rate of genotyping.
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quote continues but RIU broke
Based on analysis of known pedigrees, we have set thresholds for likely immediate family relationships. Familial relationships are useful for several reasons. Many varieties within the Galaxy have the same name, yet are genetically distinct. Familial relationships may help determine whether a variety has been mislabeled. They may also help validate pedigrees of varieties.
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Their visualization of what is a clone and what is not is unambiguous. If two cuts have the same genotype they go in the same "clone group" which has a unique "galaxy ID." That's why CSIH's Chem91 and two S1s are in three different clone groups and CSI:Humboldt's Triangle Kush is the same clone as 72 other OG Kush cuts submitted to Phylos.

Your argument boils down to Phylos is incompetent and unable to distinguish parents from their selfs because they don't publish the relationship between different genotypes at high resolution and they don't publish their methods so you can personally evaluate them. I don't think that is a very good argument. Their reasons for publishing low res diagrams may be bad but it doesn't necessarily mean they can't distinguish between different genotypes of Cannabis. ** update: they do reveal what methods they use ** Their credentials are better than yours.

There is zero reason to think that Phylos has cocked this up. This is not cutting edge science or even science. A Google Scholar search turned up a bunch of old articles evaluating loci for distinguishing genotypes of Cannabis. Here is one from 2003 asserting that "Short tandem repeat (STR) DNA markers are hypervariable and informative in Cannabis sativa." (1) This can be done by comparing as few as 10-13 loci. Compare that to the 3,000 that you dismissed earlier.

Earlier in the thread you were unaware that Phylos published anything but they publish full sequences. Someone uploaded 1000 of them into Google BigTable for easy analysis. The opportunity is there for you to put your expertise to work and show that they are incorrect in putting NSpecta's TK and 72 other OG Kushes in the same clone group. You could publish your results.



(1) https://www.bioquest.org/scope/projectfiles/cannabis_str1.pdf
 
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