Marijuana Tissue Culture Success!

drewbot said:

I fully recognize that 1M cultures is not many, but most labs have only a couple of customers. I hope you're using a distribution chain.

On the subject of varietals and intellectual property: in the United States you may only patent an asexual plant that cannot reproduce sexually. Also, you can't patent a plant that is found in nature. It must meet the statutory requirements of novelty and all of the shenanigans referred to by the general populous regarding Monsanto and genetic marking is irrelevant. For one, Monsanto did strong arm some midwesterners but had no legal right to do so. Monsanto also won some international cases that were absurd, but these new rules were clarified and unified in 2004 by the Uruguay Round Agreement if I recall correctly. Canada and the US share IP laws by NAFTA so I am confident that there would be nothing but frivolous lawsuits should the issue of IP law arise. I have a doctorate in a life science, degrees in international business, patent law, and engineering. I would be the authority on this issue in other words.

As for the varietals that I have available.. well, you are sorely mistaken. The very talented breeders/collectors are in their mid 60's now, and they have seeds that they haven't cracked for 3 decades... that is, until us in-vitro guys came around. I have a set that was made collaboratively with Amsterdam but most of mine are not. I work with collectors/breeders so I carry breed stock, very little washout (mostly washed out cannabis cup "winners"). I'm looking for Kong. I'll trade a dozen clone only cannabis cup winners for a real, original Kong pheno. It's the only Canadian that I'm truely seeking right now, but I don't have a large Canadian collection. I'm also looking for that old strain line from circa 1980 that smells like hot garbage. The original landrace was black with bright red hairs. If you were a breeder you would probably find my breed stock by word of mouth. Guess I'll wait for you to come to me

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I have 'Kong.'

I went with Bill Berry Farms tc kit. Very happy with the kit. The compiled a lil CD Rom for instructions. Getting everything started soon

WolfZen said:

Ok Mr. "Doctor" "CEO" and "President" of a company that doesn't exist outside of some links cyberspace.

I'm surprised you had time to make another meaningless promise in a response here again. You know, because I'm sure all those meetings with your "shareholders" and "CFO's" take up so much of your valuable "CEO" time. And I can't even begin to imagine your schedule as a "CEO" and a "doctor" doing "research" with all that new-fangled "cutting edge" stuff that's just way too complicated for you to even begin to communicate about with any substance to the scientifically illiterate commoners found here.

Good luck with your "business" endeavors. I'm sure you'll be a great "success".

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You love being a 'dick,' don't you? Did you fuck up a harvest or something? The thread definitely jumped around a bit, but man, these dudes are benevolent as hell. Taking the time to acknowledge you should be celebrated. Whatever you're smoking, switch it up, it makes you cranky

if you bought the kit from that vhs vidoe in the 80 turned into c.d. they will waist your money the instructions date back to the vidoe you'll get confused, i usto build houses and not any old houses i built energy efficient homes for the owners of cancer hospitals 15 restooms and i go threw the prints with the cad drawing ingenieers and wall panell ingeeniers and the city and the all kinds of shit i would redraw their prints no lie.. so i get the kit the c.d is of an old vhs the recording is low quality and the instructions on the vidoe are wrong because the chemicals are difrent and the p.h. changes in the agur you'll have to call him for a one on one.. ppm all the instruction are diffrent because of the new chemicals are premade but the instructions are also wrong a lil... make shure you are in a well still air area.. because for every dust partical in the air thier is a hundred spores on a dust partical and that could land in your shit also you'll need a steem presure cooker and you have to get it on line dont relie on the microwave also sturing the agar your putting your hands over the chemicals you should buy that mixer with the magnet.. i myself bout a green house to putinside my home i havent got around to it and a hepa filter.. i have to built the green house from harbor frieght and run it inside their i will be well disinfected and run the mix under true sterile invirment that cat doesnt have much repeat customers im shure if your smart you'll know you can get the stuff else were for cheaper than the fucken kit for realz he also sent me shit without caps if i had a gig like that idd be on it for realz change the c.d. vidoe and change the liturature.. not be like on the phone ow buy the way i forgot to tell you or write this key step.. lolz fucken joke good luck.. i might not know who to write but i know how the fuck to grow and clinics love my ass.. so its got to be tits good luck.

What ???

that kit rocks dude.
I've been tissue culturing for decades, I do not use a hepa filter within my culture "hood". I am clean, My "grow tent labs" are sterilized before any transfers, and are sealed up when not.

no air is moving. mold comes from contaminants. 9 times out of ten. I can see a hair, or lint, or plant material not properly sterilized causing this.

I use a normal canning pressure cooker, and never have issues. I am clean. my explants are clean. I rock at tissue.

It seems the only people that try it, and bitch the loudest, are the ones who failed at it. I thought at first maybe they were looking out for their bro's so they dont waste time or energy trying it...BUT NOOOOOOOO

They want to attack and bit and spit lies, like a fuggin agenda !!!! very strange man. I successfully produce over 9000 cultures per quarter dude. in a closet, and that does not include marijuana cultures ! these are the ones that 4 local nurseries purchase from me all year long.

There are great books on the subject, and why the rumors are spread. It used to be that scientists worked from their home, now its commercialized. Imagine if everyone actually believed how a genetically modified corn kernal seed changes humans at their very dna, when ingested....imagine??? the only way to know this info, is if you've done it.

STOP EATING GMO FOODS NOW. STOP USING HORMONES IN YOUR GROW ROOM NOW. CHANGE UP STRAINS, RELAX, AND IT WILL ALL WORK OUT FINE.

NebulasINblooM said:

I went with Bill Berry Farms tc kit. Very happy with the kit. The compiled a lil CD Rom for instructions. Getting everything started soon

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you are going to have a blast man !!

I bought a 15 dollar sonic toothbrush cleaner that I use with "Tween" soap, and alcohol, bleach, to sterilize my explants. this virtually stopped all contamination. good luck

I can't believe I read this whole thread and didn't learn shit.. Except some other plants can have thca that looks nasty, and I am not smart enough to even try this method, and tissue culture is kinda like growing shrooms.. I dunno maybe its because I have been drinking..... Fuck it...

pharmacoping said:

What ???

that kit rocks dude.
I've been tissue culturing for decades, I do not use a hepa filter within my culture "hood". I am clean, My "grow tent labs" are sterilized before any transfers, and are sealed up when not.

no air is moving. mold comes from contaminants. 9 times out of ten. I can see a hair, or lint, or plant material not properly sterilized causing this.

I use a normal canning pressure cooker, and never have issues. I am clean. my explants are clean. I rock at tissue.

It seems the only people that try it, and bitch the loudest, are the ones who failed at it. I thought at first maybe they were looking out for their bro's so they dont waste time or energy trying it...BUT NOOOOOOOO

They want to attack and bit and spit lies, like a fuggin agenda !!!! very strange man. I successfully produce over 9000 cultures per quarter dude. in a closet, and that does not include marijuana cultures ! these are the ones that 4 local nurseries purchase from me all year long.

There are great books on the subject, and why the rumors are spread. It used to be that scientists worked from their home, now its commercialized. Imagine if everyone actually believed how a genetically modified corn kernal seed changes humans at their very dna, when ingested....imagine??? the only way to know this info, is if you've done it.

STOP EATING GMO FOODS NOW. STOP USING HORMONES IN YOUR GROW ROOM NOW. CHANGE UP STRAINS, RELAX, AND IT WILL ALL WORK OUT FINE.

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Bill Berry's kit is legit. He supports my friend Joseph Pietri; who is the MAN for hard to find genetics .

pharmacoping said:

you are going to have a blast man !!

I bought a 15 dollar sonic toothbrush cleaner that I use with "Tween" soap, and alcohol, bleach, to sterilize my explants. this virtually stopped all contamination. good luck

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I don't believe in luck, and I would never let a microwave in my home . I have an extra tent, and grabbed a can-lite filter with a 6 inch max fan for it. I'm gonna have a go with positive pressure to keep dust out of my tent. It's a brand tent, and secret jardin is solid. I'm slowly piecing it all together, because my garden takes a lot of maintenance! I'm turning over a flower room this week, and hope to tackle this next. I'm gonna shop for a magnetic stirring thing, and a pressure cooker! The instructional video with my kit makes it all very simple! Looking forward to helping out and getting a journal going. Excited about kicking out my moms

P.S. I don't use hormones; I use amino acid boosters, and organic/synthetic base newts. Also, I've studied GMOs; not impressed. Most all of my family and friends call me before they call their doctor. I ALWAYS take on disease naturally before hittin up a doc. My boy is a physician, and I love the guy (great kid), but man, most of them have fucking ulterior motives! They are trying to get paid! In the states, physicians are only required to have 20 hours of nutritional based education! Fuck that. I juice raw veggies in the morning, take my dehydrated veggy, super-food, grass and algae shake; I FEEL invincible!

Peace

Peace,
Neb

pharmacoping said:

What ???

that kit rocks dude.
I've been tissue culturing for decades, I do not use a hepa filter within my culture "hood". I am clean, My "grow tent labs" are sterilized before any transfers, and are sealed up when not.

no air is moving. mold comes from contaminants. 9 times out of ten. I can see a hair, or lint, or plant material not properly sterilized causing this.

I use a normal canning pressure cooker, and never have issues. I am clean. my explants are clean. I rock at tissue.

It seems the only people that try it, and bitch the loudest, are the ones who failed at it. I thought at first maybe they were looking out for their bro's so they dont waste time or energy trying it...BUT NOOOOOOOO

They want to attack and bit and spit lies, like a fuggin agenda !!!! very strange man. I successfully produce over 9000 cultures per quarter dude. in a closet, and that does not include marijuana cultures ! these are the ones that 4 local nurseries purchase from me all year long.

There are great books on the subject, and why the rumors are spread. It used to be that scientists worked from their home, now its commercialized. Imagine if everyone actually believed how a genetically modified corn kernal seed changes humans at their very dna, when ingested....imagine??? the only way to know this info, is if you've done it.

STOP EATING GMO FOODS NOW. STOP USING HORMONES IN YOUR GROW ROOM NOW. CHANGE UP STRAINS, RELAX, AND IT WILL ALL WORK OUT FINE.

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you know whats funny ill tell u ya im gona bitch if i spend 250 on a kit 250 on a presure cooker all the extras and yet they dont tell you to be in a still room of air flow, that if you look into a dark room and open the curtain and see dust particals in the air thier is a hundred to one partical in the air they dont tell you to go into a tent, and they dont have the chemical all correct on the instructions because the formula changes into quote'' better formula from powder into liquid, so no im not telling you to drop it im still looking to do it its my second go at it... and yes i have failed.. but from trouble shooting i know were i mad my mastakes, i went to harbor frieght and bout a tent you could say its a green house but my house is big enough to open it, i have dogs they track in dirt and dander hair i have carpet and i have a kick ass a.c. system i live in the desert, but i would be pist off if i spent close to 800 dollars and failed they need to be real with instructions i cant guess whats happening with the powders and the liquids and they aint even labled how the fuck do you put an old resapee into liquid and then guess what the fuck to do with unlabled shit.. lolz i think its a poor kit let me go and get it since you say so, supper starter kit www.planttc.com thats what it was just like pollen you cant see it but it shure can fuck up your crop with one lil nanerd.. so unless you know the enviormet, and can put together what one c.d. seas and what you actualy get then i tip my hat to you man because you can read minds and you are aware of your enviroment the tent was a good idea see they should say that but in me i failed thats why i got the fucken green house i had to trouble shoot the hole thing i bought another book and well thats were i found out what i was doing wrong.. today im gona buy the mixer with magnet so that i dont drop dead skin over the ogar. i dont know about you but i dont like given my money away to something that is half assed lolz im shure you feel the same way..

ow and buy the way i experiment on the daily with milars with lights under and around with aroe with nutes with lighting positioning over a forest with lights flicking of and on to mimik clouds man i love to do shit like this and i have all kinds of information im sad that i couldnt do this one thing, lolz but im not gona stop im gona start before i have to leave to go.. but i love to experiment and i will finish this one thing just because you sead so lolz. ill jot down every fucken thing

dude check out the how to make a magnet mixer on youtube it s fucken bad ass for next to nothing..

ajgrowsalot said:

Is any of this even necessary? If you need so many plants you are worried about plant numbers you probably aren't growing "medically" anyway so why concern yourself with staying within medical numbers. I just seems so unnecessary to me to be growing plants in test tubes when they are so easy to grow in the first place. Sure you have to have a mother plant, then take clones (oh, how difficult) but it still seems easier, cheaper, and faster.

The science is cool but the kits that Billy Mays is talking up just seem silly.

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You are right sir, it is cheaper, easier to cut clones. But I do resent the "non medical" aspect of your tone. no probs though, let me explain?

I harvest, like clockwork, every week, A complete rotational garden, perpetually operating,flawlessly, will need access to healthy vibrant "super starts" every single week, with each harvest.
I service only five patients, completely state compliant, with dozens of choices(never over my weight limits, or plant limits, or seedlings, or clones) every single week ! My extracts are used by an oncology patients, and to treat psoriasis, glaucoma.
The strain selection cannot be hit or miss. theres way more going on here than "Whoa, that shit really stoned me"

when lab reports are returned to me, I absolutely am expected to reproduce exact results. These tasks simply could not be facilitated without tissue culture.

here is a sample menu of mine, absolutely impossible, in a legal garden in MI, without tissue culture, or outside buys. These are all grown to full potential by me, and everyone one of each particular strain is exactly the same as its sister, 1000 times over, no losses. , and like strains mature on the same day, everytime they are planted.

Huge Buds
Heavy weighted Bags
$ 225 for one ounce
and less for more !!
Nepalese Temple Ball hash- 20$ gram
Pure Honey Oil Extract= $60 gram
Rick Simpson Oil = $75 10 ml syringe, No Naptha, Butane, or Alcohol !
Top Quality Ancient Healing Topical= $25 2 dram bottle--Very Potent Healer
Next week's menu includes
Cherry Montoya out of stock
PlushBerry--------------------------------------->>
Rosetta Stone------------------------------------->> ONLY $ 225.00 PER OUNCE !!!!out of stock
100% Columbian Gold Sativa !!-------------------->> OR LESS !!!!!!!!!!!!!!!!!!!!!!!
Cherry Tahoe Og Kush------------------------------>>>
Coming soon !
Cindy 99 Pineapple Cut-----
100% GROWN BY ME. MOLD FREE, SAFE
RELIABLE, ALWAYS STOCKED.
Chemo-------------------------
OG Kush #18--------------
Cataract Kush-------------------- EVERY STRAIN IS $225 PER OUNCE !!
Presidential Kush---------------- AND LESS FOR LARGE QUANTITIES
100 %LEGAL , NO SPRAYS or OILS
Lemon Og#18----------------
Vanilla Kush----------------------------------
ALL PRODUCTS ARE PROVEN WINNERS
AND FREE OF PATHOGENS AND BACTERIA
Fugu Kush------------------------------

PHYSICIAN APPROVED MEDICATION !!
Jedi Kush-----------------------
Captain Krunch Kush-------------------------------
R.K.S.-------------------------------------------------
Holy Grail Kush-----------------------------------------
Kosher Kush--------------------------------------------
Acapulco Gold-------------------------------------------
LSD---------------------------------------------------------
Izzy-----------------------------------------------------------
Critical +-------------------------------------------------------
Banana Kush-------------------------------------------------
Golden Back Gorilla(directly from Jamaica mon!!)------ ony 225 per oz !!!

thump easy said:

dude check out the how to make a magnet mixer on youtube it s fucken bad ass for next to nothing..

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If 75$ is buggin someone, before they get into culturing, stop now !! Save money somewhere else in your hobby expenditure list. If shortcuts were consistantly successful, they wouldn't be shortcuts, they'd be the way it's done. si?

google tissue culture kit. none will do more than store a clone for you though, if lucky. but they wll get you started with many things you need, and hands on experience.

MikeHancho85 said:

roses with thc really? that maybe be the single DUMBEST comment i've read on this site. everyone here is now dumber for having read this

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you sir, are an idiot. There are plants producing human replacement tissue. tune out of comedy central sometime, and onto the discovery network, geesh

thump easy said:

come on pros i dont want to read the past i need info on the now of days kinda set up.?

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google man, google, spend 250, buy a pressure cooker, and a grow tent. follow directions and you can store a clone for a hundred years if desired, in a test tube, without roots!

@pharmacoping,what strain do you prescribe for psoriasis? my cousin suffers from the worst case ive ever seen in real life(not on tele) id love to get something to help her out!! im not in a legal country as you can probly tell!!! and what affect does this strain have on the condition does it help clear it up or stop the itchy pain?? id be very grateful if you can help me with this.CG

pharmacoping said:

Rick Simpson Oil = $75 10 ml syringe, No Naptha, Butane, or Alcohol !

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Lemme guess - pentane or hexane?

pharmacoping said:

I do this very well, and assemble kits for sale too /prepared solutions/pre filled sterile containers/proper nutrients, and even genetic specimens, for observation purposes. Some samples include Thyme, Rosemary, Tahoe OG Kush,PlushBerry,Kandy Kush, and hundreds of other non plant dna samples. The study of these samples will can prepare you for the art of tissue cultivation/cloning. From a single spec thousands of master programmed clones can be taken monthly ! Imagine no more mother plants or clones using your plant spaces? How important is that to you? These are stored/divided as masses of tissue or unusable roots. Only in the last few days do they become a "plant". You can easily have roses with thc, or mj without leaves, or even glow in the dark, really, I've seen it ! It's not the future, it's now ! Patents are already being granted for different mj types grown this way. suicide genes,inability to clone/seed..are some of the programing in these super corpopharma plants. Instructions are available online, but the right mixes are almost impossible to find and invaluable to growing your own rare herbs successfully, and storing their dna forever. With these supplies you'll learn how to master this fast because the work is done !! Plan on spending a couple hundred bucks or less, depending on the equipment you have at home...jars/mixer/pressure cooker,etc.

I'll answer some q's here, but private message me for instructions for above.
pics coming soon, soon as I figure that thing out !

peace​

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I've googled marijuana and tissue culture a few times, and this is the number one result. Like my name suggests, I'm angry. I haven't seen how this thread developed but this man is an f-r-a-u-d.

I've worked in an actual tissue culture lab, and have learned the science behind it.

• These are stored/divided as masses of tissue or unusable roots. Only in the last few days do they become a
  "plant"
  ​
  
  
  
  

This is wrong. You don't store masses of tissue, and yes, the roots are still usuable. Why? Masses of tissue means callus culture. Callus culture would cause genetic variation, and that's the last thing you want as a mj grower. You want good genetics for maximum yield, right? So WHY would you want to introduce genetic change? You would not get clones. You could induce callus culture from roots. Callus formation would be the result of wound response. It doesn't matter if it's a root sample or stem tissue because of totipotency.

• You can easily have roses with thc, or mj without leaves, or even glow in the dark,
  ​
  
  
  
  

Roses with THC? Really? How would you explain this with anything other than transgenics, if even possible? We're talking micropropagation, not mad scientist experiments. MJ without leaves? Why? Why would you not want leaves? Leaves are expanded areas of photosynthetic tissue, which is meant to increase levels of photosynthesis... I'm sure you've tried to revive plants that were on the brink of death. Doesn't happen too easily after leaf drop, right? Glow in the dark... okay. I don't think I know of any glow in the dark plants.

• suicide genes,inability to clone/seed..are some of the programing in these super corpopharma plants.
  ​
  
  
  
  

inability to clone???? There's a reason companies patent plants. Because anybody can take vegetative cuttings and induce a wound response. Inability to seed would probably indicate an uneven polyploidy level (like bananas are triploids, so their seeds abort, which is why you can eat bananas from the grocery store, but can't eat some ornamental bananas).

Tissue culture isn't magic, and it's not even that complicated. You have to have an understanding of how plants work to an extent and have to understand WHY you want to use tissue culture.

anyone doing cannabis TC at home is doing it for their own amusement, no more no less. sorry.