Has anyone tried to clone via tissue culture?

I'm talking about grinding up a leaf or stem, etc., and then regrowing the tissue on some kind of agar jelly.

If this is feasible, then it would be very easy to just grab a leaf from a really special plant and continue the genetics that way instead of hybridizing and regular cloning.

oh…and, this…


http://www.triqsystems.com/products/maia-culture.html

@Sativied

I think you brought this technique up once in one of the cloning threads?

It is possible, but not logical for cannabis where genetic diversity is better and it is very easy to clone... Unless you want to plant millions of them, like the flower industry

Dark heart nursery is trying to do this right now, check out their site

tstick said:

I'm talking about grinding up a leaf or stem, etc., and then regrowing the tissue on some kind of agar jelly.

If this is feasible, then it would be very easy to just grab a leaf from a really special plant and continue the genetics that way instead of hybridizing and regular cloning.

Click to expand...

I've been told these aren't the best resources but the outcome seems legit:
http://www.lycaeum.org/mv/anagrams/cannabis_tissue_culture.html
http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf

So short answer is yes, it's feasible. And some parts seem to produce better results than others. And it does have its useful purposes like rejuvenation of clones or revegging, or easier transport (just send leaves across the world, roll up in a pen).

"Grinding up a [...] stem" may not be the easiest way but that's specifically what I'm interested in. If you got a leaf, you probably can take a piece of the stem it's on too, in which case regular cloning is the obvious to go. The tc plantlets need time to adapt. Unless you have specific reasons or want to produce tens of thousands of clones than regular cloning is much easier and faster.

BDOGKush said:

@Sativied

I think you brought this technique up once in one of the cloning threads?

Click to expand...

@Hammerhead571 is testing or is going to test a 70-buck kit that seems good for getting your feet wet. Maybe he has some more info or a link.

The place I got the kit from sent the wrong kit. I got the big ass 220$ kit for 60$? I wont be able to test this as quickly as I had hoped. The larger kit is much more involved. I did start a few polls on this subject. From what other including breeders are saying TC is not nor will ever replace the way we clone today. Its much more useful in a lab environment.. Its way to labor intensive and the cost is much higher vs how we do this today. The thing we don't keep in mind is the Nutrient gel that is mixed up for the TC to root and thrive needs to be replaced often. Depending on the stage of development you will need to change the Nutrient gel to a different type. Every time you do this there is a chance you will introduce contamination. Right now TC is not ready for everyday cloning but if you want to tinker its fine but be ready for alot of work and $$ to keep it all going healthy...