Cloning Plants By Tissue Culture

WolfZen

Member
Read it and still have questions. Foremost being how are you planning on going from culturing bioluminescing bacteria to having glowing plants (even if it's just for fun)?

There's no need to get upset at questions being asked.
 

WolfZen

Member
I have never tissue cultured anything, or grown marijuana, or infringed upon any patents. Everything I say is a lie and every photo I post has been photo shopped a million times even. just sayin'
Be that as it may, could you answer any question directly without getting upset, changing the subject, or otherwise avoiding answering?

How are you planning on going from culturing bioluminescing bacteria to having glowing plants (even if it's just for fun)?
 

WolfZen

Member
Ok, electricity usage is top secret if you say so. What protoplast material are you referring to? From the flourescing bacteria you've cultured? How did you extract, isolate, and copy it for use?
 

pharmacoping

Active Member
...millions of dollars are spent to create GMO Fusarium fungi that kill marijuana. A former employee of an "anti-drug" agency says biotech scientists are trying to genetically modify marijuana to create zero-THC cannabis that genetically “terminates” high-THC varieties. Growers in Hawaii and elsewhere tell spooky stories about helicopters dumping GMO insects on marijuana crops…seemingly indestructible voracious insects that ate the marijuana to the ground virtually overnight.
No, those people who reported that weren't hallucinating off some Puna Gold from the Big Island of Hawaii. Those GMO insects, and a lot of other scary stuff are real parts of the GMO brave new world. It's like science fiction come true...
http://bigbudsmag.com/grow/article/discover-how-you-stop-gmos-harming-your-medical-marijuana-february-2012
 

WolfZen

Member
Yeah that's really interesting and all. But let's get back to talking about tissue culturing. You're right, it IS fun and I'm glad you have this thread to discuss it. So can you answer some of my questions?
 

WolfZen

Member
dude, I apologise for being elusive. This is delicate for me, and I simpy cannot give you the answer I know you're looking for. but it is in the book you said you already read, maybe read it again, or google. when you find it, you can post it, but I cannot.
No worries, can you show some of the results of your super secret processes with micrografting if you don't want to talk about the process itself?
 

Bonkleesha

Active Member
first i smoke 2 joints. then i take a nice plant... about 4 feet and take off all of the lateral maristems and leaves so that there is only one apical maristem shooting straight up. then i cut it out of the pot and whoop the shit out of this WolfZen character with it.

rinse and repeat.
 

WolfZen

Member
first i smoke 2 joints. then i take a nice plant... about 4 feet and take off all of the lateral maristems and leaves so that there is only one apical maristem shooting straight up. then i cut it out of the pot and whoop the shit out of this WolfZen character with it.

rinse and repeat.
No need to get upset and try to be rude. Why do you keep switching to different accounts?
 

alphawolf.hack

New Member
man forget about these guys. im in the sit. i want to learn to micro-culture but these guys aren't sharing shit. best to read as much about as you can and learn about it another way..... so far im still in the process of building hood with gloves and filters and and positive pressure and all that. first you got to learn the science part then build a small lab that can preform more functions than micro-culture than start experimenting getting the chemicals the you can experimentally micro-culture

this is the process im going about to get there i cant see any other way to do because you cant just jump right into it. even if you know all the ingredients to add to a culture it wont survive if the conditions are not correct. so i guess my idea is if you can build the environments and make the chemicals then you can culture.
 

alphawolf.hack

New Member
that bug shit is some scarey shit and of course it absolutely true. on the other hand there are better organisms to design bugs to eat.
 

Bonkleesha

Active Member
man forget about these guys. im in the sit. i want to learn to micro-culture but these guys aren't sharing shit. best to read as much about as you can and learn about it another way..... so far im still in the process of building hood with gloves and filters and and positive pressure and all that. first you got to learn the science part then build a small lab that can preform more functions than micro-culture than start experimenting getting the chemicals the you can experimentally micro-culture

this is the process im going about to get there i cant see any other way to do because you cant just jump right into it. even if you know all the ingredients to add to a culture it wont survive if the conditions are not correct. so i guess my idea is if you can build the environments and make the chemicals then you can culture.
although the gloves arent a bad idea, we have been doing our microprop. in the science building under HEPA hoods.... no enclosed gloves or whatnot. to each their own!

as far as sharing microprop info, its all in this thread. go pick it out. i even uploaded lab manuals from school, dude. have at it.
 

alphawolf.hack

New Member
im re-reading there is some useful info for sure... but i still have alot of ?'s i will just go at this by myself for now .i have alot equipment to purchase b4 i make any real statements around here. i wasnt trying to be a jerk and say to u guys wont help. but you guys are on another level with this shit its pointless for him to come in here without any prior knowledge and expect you guys to give some real answers. because those answers probably run a page or so long and are somewhere else already written for him. im the same way with electronics i know so much shit it would make your head spin. its like asking how do i use a capacitor? there is a long answer to that along with alot of variables and possibilities.
 

nitro20%

Active Member
DUDE,go buy a kit,,, im tellin ,,,, that if you dont,,,,, you will buy a half of one before you could even split something...I WOKE UP 3 WEEKS AGO AND DECIDED TO TC,, DONT ASK WHY I DONT KNOW....DIDNT KNOW SHIT BEFORE THIS!!!

GO BY A KIT AND FOLLOW DIRECTIONS,,YOU ALREADY KNOW TO BE CLEAN,, YOU ARE HALF WAY TO YOUR FIRST AGAR FAILURE... SERIOUSLY.....I THINK I GOT LUCKY...BUT KNOW IM INTO IT,,,BUT IM INTO IT FOR 400+ AND I HAD 500$ WORTH OFF STUFF TO START WITH.. SO TAKE THE INFO THATS HERE AND DO WHAT I AM NOT,, SHUT UP AND LISTEN,,,I/WE WILL DO THE REST....UNLESS YOU HAVE A PAGE LIKE MY CHINA MEN PAGE.....I SAY THIS WITH A HUGE CLOUD,,AND A BIG BONG SMILE!!!:bigjoint:
 
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