Cloning Plants By Tissue Culture

pharmacoping

pharmacoping

Active Member
alphawolf.hack said:
whats up pharm. hey man okay so i just finished book 3 on this subject got a couple a ?'s for you... do you sterilize the media nute hormone ect.? or just the container? isn't all that stuff supposed to com prepacked and sterilized or is this just a safety measure? also once you reach the stage where plantlet is cultured in hormone free media how long is shelf life? as far as degrading genetics does this only apply to callus cultured cells? damn i learned alot.

bankleesha you werent around he said togo4it.
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sterilize the whole mix. dont tighten the lids on the containers in the pressure cooker, until it cools naturally. I dont culture in hormone free media until in the vegging room. callus production division/rooting chuting/hardening off, then to veg.
 
pharmacoping

pharmacoping

Active Member
Bonkleesha said:
HEY, DUDE! i got my test tube from the microprop lab (the chuting one.. we transferred our new chutes into the rooting media). anyways theres like 2 inches of media in it. if i am careful and sterile, and i take about half an inch out where my miniature rose was and use it for cannabis?
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Do It ! Do it now !!!! for real, should be cool.
 
pharmacoping

pharmacoping

Active Member
old mother/new mother, either one....never heard of fruit fresh in tissue culture??? nutrition=ppm=between 150-200ppm. plus some sugar.
 
pharmacoping

pharmacoping

Active Member
El Ray,

Your a couple years late on the pineberry, it's kind of in the past.
I dont own a microwave, no comments on using it.

If shortcuts are on your mind at this stage, you're in for a ton of moldy jars, and un usable plant material. Follow the directions exactly, to hope for similar results described in the kit, ohterwise its all experimentation, which is cool too !!
 
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el rey

Member
is the pineberry strawberry plant available here in US ? I could not find it anywhere.

No shortcuts are in mind, just have a microwave but don't have a pressure cooker. But I will go buy one no prob. Can't wait to get started. Is there a special media mix for cannabis? Like more of one thing than another?

pharmacoping said:
El Ray,

Your a couple years late on the pineberry, it's kind of in the past.
I dont own a microwave, no comments on using it.

If shortcuts are on your mind at this stage, you're in for a ton of moldy jars, and un usable plant material. Follow the directions exactly, to hope for similar results described in the kit, ohterwise its all experimentation, which is cool too !!
Click to expand...
 
pharmacoping

pharmacoping

Active Member
thats pretty strange. gassing, dont ever sniff cultures, no matter how tempting. these molds are really nasty. my first guess would be some sort of strange mold/fungus/pathogen/bacteria taking hold. I'd bleach it and dump it. I saw live animal/human tissue do this before, maybe an eye lash,skin flake,not sure? toxic though..
 
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el rey

Member
pharm i did it! Bammmmm....i have roots starting on a leaf! They were in my green formula i'll post a pic when i can focus on the roots iphopne cant shoot past jar.
 
pharmacoping

pharmacoping

Active Member
thats tits !! the most difficult part of a plant for me to culture is the leaf. I definately want to watch that one !!
then try that same formula on all the other parts too, and compare ......man, life is so short
 
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el rey

Member
IMG_0122.jpgKind of hard to see but the white things are roots poking out and from the looks of the other stuff it looks like a callus is forming? Or is it mold lol. The white things look like spikes coming out (roots) and they even have tiny hairs on some of them. Whats everyones thoughts on this?
 
pharmacoping

pharmacoping

Active Member
this looks like rooting callus ,aterial, with some mold trying hard to win. If it is mold, it can be washed again and transferred. older samples have abetter chance of surviving the mold
 
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el rey

Member
awesome! so do just pull it out of the media and rinse in 10% bleach like i did before? Or go weaker? Will it kill my callus? Could i just take a small piece of the callus and transfer it to new medium to start one mold free and have more success to get growing? What would you do now? I have another strain that has a callus as well.
 
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