Cloning from Fan Leaves

This is my first crop. and I am 6 weeks into vegging. I have 2 Hawaiian Snow and 1 Early Fatty. I cut a fan leave from each and they both died in like a week. What is the best method for getting clones. If the Fan Leave doesn't work?

Hawaiian Snow in Colorado

raydenvader420 said:

This is my first crop. and I am 6 weeks into vegging. I have 2 Hawaiian Snow and 1 Early Fatty. I cut a fan leave from each and they both died in like a week. What is the best method for getting clones. If the Fan Leave doesn't work?

Hawaiian Snow in Colorado

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Hi All.
Hiya m8, think you've slightly the wrong idea about doing the leaves, this is quite an advanced technique, coming under the heading of tissue culture, one really needs a very clean environment and specialized tackle, the very least I would imagine would be sterile soil or nutrient medium.
The idea is to remove a leaf, (in this case, although more advanced tissue culture can also be done with small bits of leaf and stem too), cut accross the leaf viens in a number of places, and lay the leaf flat onto the media. in some plants at the cuts small plantlets will form and each one will be a baby plant. I cannot imagine this being a very quick way though and really is not needed with weed, which is easy/very easy to propagate with cuttings in the usuall way, (strain dependent obviously).
If they are 6 weeks from seed you may be a little early for taking cuts, if I were you I'd carry on vegging until ready to flower and then take a few of the smaller bottom branches for cuttings, and carry on and flower the plants.
There are plenty of good cutting tutorials around, cheque out the forum FAQ for a start, you shouldn't really have any probs.

NotMine said:

all the bs aside it works well big clones dont root well due to tissue structure in mature stems listen to the botanist...stupid people amaze me theres lots of plants harder to grow than weed its a.....weed it will grow in clay with no water for god sake relax and smoke a green one

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Hi All.
Absolutely M8, as you say the more woody, the less success you'll have with cuttings, this is where air layering would become the prefered technique, (wich BTW there is a thread thats almost turned into an air layering thread, here:- Anyone ever tried this? Crazzzy ).
But as you so rightly say, this shit almost roots on its own, compared to some plants.

Toodle Pip

this thread is full of some of the angriest posts ive seen on rollitup over something so harmless.. isnt weed supposed to make you happy not mad? everyone needs to just go smoke another bowl.

So far after 33 pages, albeit some good points and people trying to help this thread, I havent seen one constructive point that would favor trying the method the late great elusive genius dr. woo has so cordially provided us with. I have however seen missinformation caused by it and yes that does make me mad, not so harmless when your filling less experienced growers heads full of shit ( Id assume the population on the site would be more inexperienced people needing information). Provided they should get an accurate book on the cultivation of the plant there choosing to grow AND provided they should have attempted to read through the muck of the entire post, it still hurts to see little billy first grow finishing his garden with dead cloned fan leaves for crop #2...

Some asshole replies perhaps, but do you think the flat earth society didnt want a little proof before sailing their boats past the waterline in the ocean? Or the painters with there sexy camel hair brushes??

If you come on a reputable site to make an outlandish claim that you can do what no one has and in a time frame that is fast(almost) as whats currently considered the most viable solution to perpetuate crops, providing no proof whatesoever the entire time, and then cry and take your ball home when the kids on the playground laugh, you shouldnt expect much more than the replies recieved.

Almost a year later, no proof, no word, the only help to his case by the posters who got up tried to accomplish what he said was possible for themselves. I think a hobo would have had enough time to buy a camera from saving just the pennies he begged for at this point. Don't want to be critiqued? Dont pretend your jesus.

hmmm... most of us would have pics of our growing clones by now...

i saw a picture of a leaf that was growing hairs and budding in a book.

R U FUCKING KIDDING ME!!!!!!! i read through all this bullshit and no fucking pics of any fan leaf clones with nodes or nuthin just sum stupid looking set up with fuckin rocks holding em down!!!!!! delete this thread as to not get anyones hopes up about seeing the fucking botanist and his mad method. GREAT THREAD 31 pages of argueing.

CLONING PLANTS BY TISSUE CULTURE

by Michael H. Renfroe, Ph.D.


Many plants are cloned by tisssue culture techniques and sold commercially. Some of the ferns such as Boston fern and staghorn fern are propagated through tissue culture. Also, many varieties of African violet are propagated asexually by tissue culture.

We can take a leaf from a plant like the plant below.

The leaf is then cleaned of contaminating microorganisms, fungal spores, small insects or whoever might be on board.


Continue to Part 2

CLONING PLANTS BY TISSUE CULTURE

by Michael H. Renfroe

Part 2
The leaf is then cut into small pieces in a laminar flow hood that provides a clean working surface. The small pieces of plant tissue that are cut out of the leaf are called explants. Below you can see what they look like.

The explants are then placed on a chemical medium that provides nutrients for the plant tissues to grow and usually some plant hormones to encourage development of new organs from the plant tissue. Below is an explant that has been placed on a chemical medium inside a test tube.

If you look at an explant with a scanning electron microscope, it would look like this.

From this explant, new shoots would start to develop. Before they were obvious to you, as they just started to develop, they would look like this with the scanning electron microscope.


Continue to Part 3
Back to Part 1

CLONING PLANTS BY TISSUE CULTURE

by Michael H. Renfroe

Part 3
After six to eight weeks, the explant will develop new shoots, as below.

These shoots may be cut free from the explant, and placed in a larger container on a new medium that will help roots to develop.

The rooted plant can then be transferred to soil. At this stage, the humidity must be kept high until the plant can adjust to the new surroundings. This process of adjustment is called acclimatization, and involves the growth of new leaves that will function in the less humid room air.

The cover is slowly opened more and more over a two week period so that the plant can gradually adjust. Then the cover can be removed completely and you have a new African violet plant.

From one original violet, you may produce hundreds of genetically identical plants.

Because the plants are genetically identical, and are of similar developmental age, they tend to produce flowers at the same time. This is very important to someone who is growing the plants and wants to get them to market just as they start to flower.

Many flowering plants are propagated this way. I hope you enjoyed learning more about how plants may be cloned using tissue culture.
If you want a more detailed explanation, please visit my Getting Started in Tissue Culture web page.
Back to Part 2
Go to beginning of Cloning Plants by Tissue Culture.

yes i have done this, it works very easy and it took 4 -5 weeks to get roots, HOWEVER, it works great for that "o shit" i wish i had a clone of this plant but its gonna be cut down in 2 -3 weeks situation. it will be the way we all do it in a few years, my child hood friend works for a produce lab and they are doing tissue culture and getting roots in 2 weeks, of course a "lab" is way differant than my grow room, however i'm getting my room close to lab status.

What passes for a laminar hood in your room? Are you set up well enough that you'd feel safe working with scary amounts of say H2S?

Its truly sad at the way people treat others in this forum.

although i have always heard that growing a "bud-producing" plant can only be done from a growth tip, i would like to see SOME PICS!!! but, after reading about the procedure, i would never use it. why would i want to go from getting roots in 7 days, to 14 days? interesting sounding, but not worth using.

Has anybody who has confidence in their cloning ever come across genetics that simply refused to root via conventional cloning? Thats a major reason the method is used with other plants..
Personally I dream of being able to grow a clone from a chronic chronic bag whose genetics are otherwise unobtainable..
Point is though that all plant tissue is capable of undifferentiating and becoming a different kind of tissue..

well did anyone actually managed to make a leaf clone form any NEW GROWTH

it can be rooted pretty easy, but how can it form new growth when there are nodes to spring out of?

maybe the rooting hormone can be applied to the top part of the plant as well, and what forms roots under the ground can form new growth above the ground?

Like I said, you may need to top it of sorts to compell it to grow beyond what it has.. Not unlike the result of basic topping, but rather than taking a miniscule portion of the total vegetation, you'd need to chop virtually all of it..

Crap... 34 pages for nothing.

all that and not one fucking picture