Tissue culture

Turpman

Well-Known Member

• Jul 24, 2018

• #1

Anyone using tissue culture? I'm doing some experiments. Thinking it may be a decent way of storing genetics. Regular cloning is the way to go for propagation at most levels. It's a pretty small package to keep strains in. I'll get a pick up later today of some success.

 

letstry

Well-Known Member

• Jul 24, 2018

• #2

Very interested to see this. Iv only seen it on the medicinal level in million dollar setups.

 

Miyagismokes

Well-Known Member

• Jul 24, 2018

• #3

One of these days, I'm gonna build an outbuilding for a lab...

 

Turpman

Well-Known Member

• Jul 24, 2018

• #4

Only one in TC so far, has been a while since I have cultured anything. RQS
I did pick up some multiplication hormones. May give it at try just for shits. They are not heat stable so I am going to have to filter sterilize the hormone into cooled media which I haven't done before.

I used to TC carnivorous plants. Multiplication hormones makes literally thousands.

Even flower in vitro.

 

Miyagismokes

Well-Known Member

• Jul 24, 2018

• #5

Turpman said:

Only one in TC so far, has been a while since I have cultured anything. RQS
I did pick up some multiplication hormones. May give it at try just for shits. They are not heat stable so I am going to have to filter sterilize the hormone into cooled media which I haven't done before.

I used to TC carnivorous plants. Multiplication hormones makes literally thousands.

Even flower in vitro.

Click to expand...

Nice. Particular fan of the baby food jar.

 

Turpman

Well-Known Member

• Jul 24, 2018

• #6

Ya they are nice to see through and are reusable win win.

 

C

CBDguru

New Member

• Jul 26, 2018

• #7

As per my knowledge, tissue culture is a collection of plant cell maintaining techniques. But I am not using tissue culture and have not much idea about it. I will love to know more about it as it is very interesting.

 

Last edited: Jul 26, 2018

Kami Samurai

Well-Known Member

• Sep 21, 2018

• #8

About to read up on this.

 

SCJedi

Well-Known Member

• Sep 27, 2018

• #9

There are simple kits you can buy but they are pricey at ~$150.

If you don't know much about sterilization technique you may struggle. Go get your supplies cheaper at phytotechlab.com. MS powder to make a Liter of media cost $2.

I want to use TC technique (Really microcloning) to try and rejuvenate some old genetics.

 

Turpman

Well-Known Member

• Sep 27, 2018

• #10

I have read it works well to store genetics too. Haven't got that far though.

 

Bodyne

Well-Known Member

• Sep 27, 2018

• #11

phinnestcannabis on IG has some insane pics per small and large grows and plants from tissue culture, I had no idea. And those plants look gorgeous.

 

somebodyhearted

Well-Known Member

• Sep 27, 2018

• #12

why use such a large piece of cannabis to culture, a small bit of any part of the plant will suffice. The downfall for hobbyist is sterile technique, and with a large plant piece like this the surface are available for contamination are exponentially increased thousands of cm compared.
the plant material needs to be cleaned also, with bleach and alcohol first I think, yours looks healthy green and alert, likely unbleached?

 

SCJedi

Well-Known Member

• Sep 27, 2018

• #13

I emailed Carol M. Stiff, Ph.D. at www.kitchenculturekit.com. She provided a workshop handout which I have attached as well as a calculation worksheet for those interested.

She said that she has a lot of experience trying to TC cannabis and claim different strains like different conditions but she will provide some references later. I will post them here.

 

Attachments

• workshopHandoutKCKWorkshopReadyMay07_2018.pdf

  2.7 MB · Views: 38
• planthormoneCalculationsKCK.pdf

  419.8 KB · Views: 81

Turpman

Well-Known Member

• Sep 27, 2018

• #14

somebodyhearted said:

why use such a large piece of cannabis to culture, a small bit of any part of the plant will suffice. The downfall for hobbyist is sterile technique, and with a large plant piece like this the surface are available for contamination are exponentially increased thousands of cm compared.
the plant material needs to be cleaned also, with bleach and alcohol first I think, yours looks healthy green and alert, likely unbleached?

Click to expand...

Ya you will not do well if you don't follow sterilization protocols. 15% bleach for 15 min 3 rinses in sterilized water. I should have taken a smaller one, but practice practice........

 

SCJedi

Well-Known Member

• Sep 27, 2018

• #15

I have a dozen reference papers that Carol just sent me. If anyone wants details on cannabis specific techniques sent me a DM and I'll get them zipped up and into a fileshare.

 

Bodyne

Well-Known Member

• Sep 28, 2018

• #16

phinest cannabis tissue culture, impressive
https://screenshots.firefox.com/m9pljZMlCm4UlIg6/www.instagram.com

 

SCJedi

Well-Known Member

• Sep 28, 2018

• #17

Phinest is in the next county over from mine. They are in Yolo.

 

Bodyne

Well-Known Member

• Sep 28, 2018

• #18

SCJedi said:

Phinest is in the next county over from mine. They are in Yolo.

Click to expand...

what's in the water, I mean, heard talk a long time, you guys are really makin it happen in that area, kudos

 

klx

Well-Known Member

• Oct 3, 2018

• #19

SCJedi said:

I have a dozen reference papers that Carol just sent me. If anyone wants details on cannabis specific techniques sent me a DM and I'll get them zipped up and into a fileshare.

Click to expand...

That would be appreciated!

 

SCJedi

Well-Known Member

• Oct 4, 2018

• #20

https://www.dropbox.com/s/691iupk3k694uqi/reworkshophandout.zip?dl=0