nickelz419
Active Member
Ya i just ordered a LC.
100 jars.
- Thread starter nickelz419
- Start date
nickelz419
Active Member
Agar ... wouldt i need a sample and a hood flow? Im starting from scratch... wouldnt lc be the best?
tylerrrrr
Well-Known Member
You could clone a mushroom with a piece of tissue,use spores or a colonized grain. A flowhood isn't required and a glovebox would do fine. The problem with LC's is that you don't know if it is contaminated until you noc up a test jar. With agar you would know within a day or so if there is any contamination. If you don't mind testing each batch of LC you make then you could just stick with that. Another thing with Agar is you can isolate promising growth characteristics.
Here is a good video on Agar.
http://www.mediafire.com/download/w3m59na8dc93dee/Let's_Grow_Mushrooms!_2.6_-_Lets_Learn_Agar.mp4
Here is a good video on Agar.
http://www.mediafire.com/download/w3m59na8dc93dee/Let's_Grow_Mushrooms!_2.6_-_Lets_Learn_Agar.mp4
nickelz419
Active Member
I plan on doing agar but i only have spores. Can i do agar with syringe spores?
canndo
Well-Known Member
That pattern is more for identification of various and different spores or bacteria. All the guy needs to do is squirt a drop or two in a plate and roll the plate around so the liquid spreads a bit. the spores will settle out after a day or so, and the liquid will evaporate soon enough, he may find that the spores have already germinated when the liquid is dry and he will see tiny spots of white all over the plate. If they look liquid, or quickly turn any color at all - then the syringe is contaminated - or the method is faulty, usually you can tell pretty quickly.Here is a good image on how you should "Swipe" your needle when applying spores.
nickelz419
Active Member
I inoced 500 ml LC ... and it sucked in my whole syringe. I then plastic wrapped it. Im not sure if this is right
nickelz419
Active Member
Ya. There was like 7 ccs. What temp is good?
nickelz419
Active Member
I have some cakes that are bout 85% col. Should i wait till 100? Or can i mixed with shit now?
nickelz419
Active Member
I am. Inoced grains 6 days ago. Lc 3 days ago. Its the jars i started 3-4 weeks ago. The pics are on my profile. The ones i thought might have a prob. Just thiught mixing would help speed.
