Thanks eDude. We are always trying to keep it simple and successful. You may be interested in one of our simplified methods set up after so much travel and TC presentations...liquid tissue culture starts. Here is a short write-up and pics. Good luck.
Hi Native. It would be my pleasure. The instructions are the same and we will be producing a cannabis tissue culture kit as soon as we get the legal "go". Here are some TC cannabis pics of the kits in action and a tip sheet for making starts in liquid medium. The media in the Microclone kit...
In tissue culture, plants can be created from leaves but it is best when those leaves have been produced themselves in tissue culture. the auxins in the medium seem to create callus tissue and roots in the ordinary course of TC cloning. Sterilizing leaves to put in tissue culture most often...
How has that worked out? The kit was assembled for $250 as a bargain to going to the trouble of building one's own kit. Everything in the kit is vital and users are encouraged to build onto it and source new needed items, particularly vessels like baby food jars. Everybody on this list should...
It is being tested at this moment using Iranian Autoflower varieties. Will let you know how it works out. Seed starts will be introduced into culture and the donor plants and cultures grown under 18 hours light. Will the tissue culture plants flower in culture even though they are so tiny...
The first Microclone grow racks held multiple vessels of over 30 varieties on one 2x4 ft shelf under two 54-watt t5's.
Regular clone ROOTING is much faster because of the resources put into creating the 6" + cuttings. We still always have some rooted clones to back up the cultures and prove...
The protocol for cannabis has been used effectively and sold commercially for over ten years now. Wang et al published the first papers because they could do the research in China. Microclone tissue culture kits are sold in hydro stores and online at planttc.com to medically and recreationally...
What a great space. I am in CO as much as I am at home these days. Your space can make all the clones you need and you will likely still have space left. You will need a filtered air area for growing and handling, kitchen for media prep and dishes, and rooting trays and racks. OUr biggest...
A typical wash is 10% bleach for 15-20 minutes, however, we much prefer 500ppm of dichlor because the Cl evaporates and typically does need the additional sterile water soak. Dichlor is included in the Microclone TC kits.
Four and six ounce polypropylene and glass jars are best for gel culture. We are also using 23mm plastic tubes for introductions and starts. All are included in the Microclone tissue culture kits. planttc.com
Make it easy and use 1-1/2" clean vegetative branch tips.
Check out our videos for Microclone tissue culture. We do coffee for fun in our workshops from cuttings and from seed. We created the kits in 2005 when we realized we could add food grade preservatives that make tissue culture very successful at home. I have been growing tc plants in my...