Schwaggy P's Random Stuff

Schwaggy P

Well-Known Member
I would like to share my method of pollen collecting and targeted single branch pollination. If you decide to replicate this process for yourself, please be warned that having a male dropping pollen requires great care. I have organized this information into 3 sections: Flowering the Male, Collecting/Storing Pollen, and Pollinating a Single Branch.

FLOWERING THE MALE
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I usually take clones of males to keep their size manageable. Once they are rooted, I plant them in a 3” square pot and put them in a clear storage tote (pic 1). You will want to keep the tote lid slightly ajar to allow for air exchange.

Where to keep him depends on whether you have a free tent, separate veg space, or just another room with sunlight. For times when all tents are taken, I keep the male in my veg area (pic 2). It is important to remember that he will be separated from the flowering females.

:idea: In the corner of your veg room or peripheral splash over light is just fine to trigger the male. There’s no need to put him 3” beneath a 1000W DE, I’ve flowered males from the spillover light of a T5.

If you have a single tent with females flowering, the ambient light in another room is enough to accomplish our task.

The 12hr dark cycle is achieved by placing a box over the male’s clear tote (pic 3). This 12/12 box covering will trigger and maintain flowering through the entire process until you’ve collected enough pollen.

:idea: Make sure the box is large enough to cover your storage tote before you begin.

If you are triggering a male for a one-time pollination without storing pollen, then timing will be important. Males will usually develop flowers pretty quickly relative to their female flower setting counterparts. Since we want our females to have a decent flowerset to accept pollen, we have to give her some time to develop them. With most strains, weeks 3-4 will give us the best window to have both nice flower setting and early enough to give the seeds time to mature.

:idea: Don’t trigger your male until about day 10 of flower for your females. This gives females the head start to ensure there will be nice sized buds to give you seeds, as well as not have to deal with and worry about a male dumping tons of pollen too early.

This 12/12 box cycle will continue for the duration of pollen collection.

:idea: You want to approach most things in this process slowly and carefully. There is no extra credit for speed. Being gentle and deliberate with him will ensure any pollen stays within the tote. Pretend you’re doing tai chi at the senior center when you interact with him.

As you gain confidence and success with this process, you can flower multiple males simultaneously (pic 4).

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Keep in mind, the goal here is to be aware of his maturity in order to collect pollen long before he creates dust storms. At first, his flowers will be small balls (pic 5) that are not much of a threat to your females at this point. As they begin to develop, you will notice that they cluster and start to hang (pic 6). Usually this timeline window can be from 10-20 days. As the male flowers begin to individualize from the clusters you are now on pollen watch.

:idea: This is the point at which you will need to be very careful about moving him around and watering him.

Once you begin to see the male flowers open, the yellowish anthers will be visible (pic 7) but not drop pollen just yet. The pollen is only hours away at this point, so remain vigilant. If you try to collect pollen as soon as the male flower opens, you’ll find no pollen freely drops, so wait until you see the anthers go from a tight smooth sheen, to bloated matte yellow (pic 8 ).

If you find that you are having issue timing the pollen drop, you can wait until one of the flowers releases pollen onto a leaf below (pic 9).

The male flowers will reach maturity at different rates, which we can use to our advantage. The older male flowers will be first to drop and keep in mind we are only needing a few flowers worth of pollen in order to pollinate multiple single branches. By collecting the first few pollen drops, we are containing the threat of stray pollen.
 
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Schwaggy P

Well-Known Member
COLLECTING & STORING POLLEN
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Now that our male has begun dropping pollen, we can start our collection. I like to use a piece of glass (taken from a dollar store picture frame) and a coffee stirrer to free and collect the pollen.

:idea: Make sure to kill any large air movers that may disturb this process. The only air circulators you may want going is an extraction fan that can create the negative pressure to keep any stray pollen inside of a tent.

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You will position your glass under the flower that is ready to drop, and just slightly agitate it with the stirrer. A slight small nudge is all that you need to free the pollen (pic 10,11).

Then, you will slowly use a razor blade to transfer the pollen from your collection surface into a storage container (pic 12). I use narrow necked glass vials.

:idea: Remember: we only need to collect 3-4 male flowers worth of pollen to have several single branch pollinations.

If you have no intention of collecting pollen for future work, then this single pollen collection is all you need to dust some branches (pic 13). So at this point, the male’s job is done and we can dispose of him long before he starts to really open up and create a high risk for accidental pollination.

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You can use any smooth surface to collect the pollen. When there are hard to reach male flowers, I switch to a plant tag for collection (pic 14,15), so just be creative with what you have.

:idea: When you trash the male, be sure to change your clothes and shower before going near the flowering females. While there won’t be much pollen this early, it’s better to be safe than sorry.

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Sometimes, I like to let the male flower out for long periods for larger stores of pollen and to make sure he doesn’t hermie later in flower. You can see the risk factor for accidental pollination escalates quickly at the male’s later stages, as he dumps tons of pollen. Ideally, your male will be long gone before this can happen (unless you want it to happen )

With your collected pollen (pic 13), you have 2 choices:
  1. If you’re doing just a few branch pollinations, the staggered timing of triggering the male, means your females are ready to be dusted and there is no need to worry about storing the pollen. You could move on to the section (Pollinating a Single Branch).
  2. If you would like to store the pollen, allow the container to dry overnight in a safe place away from fans. This allows any residual moisture to be purged for long-term storage.
:idea: Water is the enemy of pollen. Try not to get any plant material in your container as it can sweat moisture into your pollen and render it unviable. You can use this to your advantage, keeping a spray bottle with water on hand can allow you to spray around his tote if you fear any pollen may have escaped.

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The next day, you can cap your container and place it in a labeled bag with a small desiccant for long term storage. Make sure to include the date the pollen was collected so that viability can be tracked. I like to prepare single-use vials that have just enough pollen so that when I take out and use it, there is nothing left. I don’t have to worry about freezing and defrosting the same container.

:idea: I try to use up pollen within a year of its collection. As the viability begins to drop off, you’ll notice fewer pollinated flowers as you continue to use the pollen. You’ll want to refresh your pollen bank as you notice the reduction in viability.

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You can keep your packaged pollen containers in the freezer until you are ready for pollination. I keep a drawer in the freezer full of various pollens. Whenever I have female plants that are ready for dusting, I can make several crosses without having to constantly juggle multiple flowering males.
 
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Schwaggy P

Well-Known Member
POLLINATING A SINGLE BRANCH
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Now that we have our pollen collected, we can pollinate females that are ready. You’ll need:
  • Paper bags
  • Tags
  • Brushes
  • Pollen
  • Stapler
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When you have a chosen female, remove her from the flowering space into a different room to administer the pollen (pic 16).

Choose a branch from the mid to lower section of your female that will receive light through the canopy (pic 17). Choosing a branch that is not tangled with others and has free range of motion will make this process easier.

Make sure the branch you choose and the buds you plan to pollinate will fit inside of your paper bag (pic 18 ).

Affix your tag to the chosen branch just below the last bud you can fit into your bag (pic 19). This will help you at harvest time to know how much of the branch to cut to separate from your sensi bud.

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With all fans and air circulators turned off, you can begin the pollination process.

:idea: When using a freshly clean brush, the pollen will have a tendency to fall from the tip. Gently tap your brush into one of the targeted buds to get some resin on your brush BEFORE you collect the first bit of pollen onto the brush. That little bit of stickiness from the resin will make sure the pollen doesn’t fall from the brush.

We only need the tip of the brush to have pollen, this keeps the pollen within our control and is plenty to achieve our goal. Try to load your brush against the wall of the container first (pic 20). When you load the brush from the pollen pile, try to get just enough, no need to dive into the middle of the pollen (pic 21).

With your brush, slowly and gently poke your female flowers with the first few brush movements (pic22) this ensures the pollen payload isn’t flicked into the air by brushing it. Once you’ve done a few pokings around the bud, you can gently brush the pollen to “color –in” the bud.

:idea: A little goes a long way, so you really do not need more than 2-3 brush tips of pollen for each bud site. Don’t think you need tons of pollen because excess can cause accidental pollination if it goes airborne.

With the pollen fully dusted, the paper bag can be placed over the branch (pic 23). The corner of the bag is then folded over and stapled (pic 24). With the branch bagged, all you need to do is secure the bag, this will depend on your particular method of support (pic 25).

:idea: Make sure that the paper bag you choose does not have a wax coating on the inside. This coating can inhibit air exchange and cause problems.

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With your female branches dusted and bagged (pic 26), you can return her to the flowering space (pic 27). The bag should remain on the branch for 5-7 days. After about a week, you can remove the bags and find a branch that is fully pollinated (pic 28 ).

:idea: If you are worried about stray pollen after the bag is removed, you can take the female out of the flower space, remove the bag, and lightly mist the branch with water to deactivate any stray pollen.

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We will allow the females to finish their flower cycle and harvest as usual. During chop, I cut the seeded branches at the tag and hang them separately (pic 29). You can use different pollen on the same plant (different branches) to achieve multiple crosses using the same female.

Picture 29 shows 7 different crosses from one tent. When the branches have dried, you can shuck the seeds and allow them to dry/cure (pic 30). Each branch (depending on size and strain) can yield between 150-300 seeds, giving you a nice seed drop of your own creation.
 
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Schwaggy P

Well-Known Member
What are your concerns with males that might throw pistils later in flower? To date I've not seen anything to suggest this is something to be concerned with. Considering the ladies you're using will do the same.
My concern is not of a late pistil set at the end of his life, just as a female with a late nanner isn't a big deal. I'm making sure that in weeks 4-6 he isn't putting out female flowers in various spots. My usage of "later flower" is relative to how long males are usually kept (just enough to get pollen).
 

outliergenetix

Well-Known Member
Chemdog Comparison
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Here are the two Chem '91 cuts in soil with some LST.
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Hydro side-by-side
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well god damn this is a treasure trove. chemdog is my jam, and i never grew a legit one, i only grew the hso fems, but man did it taste and smell so good. ever since then i always wanted to find some legit crosses with skunk va 91 or some s1's or f1's if they went around. speaking of him someone on insta had skunk va 91 x super skunk. i thought he said the suer skunk was lost. any info or chance that is legit or is someoen blowing instagram smoke up everyine's ass? i cannot even rememebr who it was tbh
lol, you are like a chemdog archiver it seems. D, 91, andthe contorversial jb cut lol impressive :)
i believe boghi used the jb cut originally before the story was known. i think that is the story anyway
 
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outliergenetix

Well-Known Member
With credit to @vostok on this...(did I get it right? Drop a pic of that in use again please)

Interesting way to collect pollen.

Cut the bottom off a 2 liter pop bottle - small hole drilled in cap.
Carefully cut a close to pollen dropping male branch to fit in that bottle - Put the cut end of the branch stem through the hole in the cap.
Place the whole set-up into a glass just full enough that the cut stem end is in the water.
The branch will pollen drop over the next cpl of days. Lightly shake the bottle and the branch will drop more pollen.

Collect, being sure NOT to get any pollen wet at all! (Did it by cutting off the wet end of the branch first.)

Store in the freezer with a desiccant bag or pack......Rice - does NOT work to prevent moisture.

Always allow container of pollen to reach room temp before opening! Otherwise you risk pollen being killed by condensation!

Store for 6 months safely. After that, the viability deceases over time.....


You got a Bitch'in series going here Swaggy! Garden too!
are you culling the rest of the male plant you took the branch off of? or do you have a dedicated seperate room the males stay in all the time?
 

Schwaggy P

Well-Known Member
well god damn this is a treasure trove. chemdog is my jam, and i never grew a legit one, i only grew the hso fems, but man did it taste and smell so good. ever since then i always wanted to find some legit crosses with skunk va 91 or some s1's or f1's if they went around. speaking of him someone on insta had skunk va 91 x super skunk. i thought he said the suer skunk was lost. any info or chance that is legit or is someoen blowing instagram smoke up everyine's ass? i cannot even rememebr who it was tbh
lol, you are like a chemdog archiver it seems. D, 91, andthe contorversial jb cut lol impressive :)
i believe boghi used the jb cut originally before the story was known. i think that is the story anyway
Glad it can be helpful. I believe Skunk VA mentioned in his Pot Cast episode that the Mass Super Skunk is still around. I figured people would really like to have some pics of the 'Dogs to be able to compare their hybrids.

Bodhi did use the Chem '91 JB with the understanding that it was THE Chem '91, until it was known to be not the case. Though in his defense, he had Chemdog (the guy aka "Mass G") verify some of the flower as the original '91 and Bodhi later made a statement to the community with full disclosure.

epic album!!!!... prodigal son, twillight zone, another life, innocent exile, purgatory....... oh my!!!!!
paul di'anno may be the greatest singer nobody knows,
Agreed! I'll use my vinyl collection for seed picture backdrops, I have more Maiden.
 

outliergenetix

Well-Known Member
Glad it can be helpful. I believe Skunk VA mentioned in his Pot Cast episode that the Mass Super Skunk is still around. I figured people would really like to have some pics of the 'Dogs to be able to compare their hybrids.

Bodhi did use the Chem '91 JB with the understanding that it was THE Chem '91, until it was known to be not the case. Though in his defense, he had Chemdog (the guy aka "Mass G") verify some of the flower as the original '91 and Bodhi later made a statement to the community with full disclosure.


Agreed! I'll use my vinyl collection for seed picture backdrops, I have more Maiden.
weird i thought i heard on that same potcast episode it was lost lol. i musta misheard or remembered it the opposite. good to hear. and yea, i remember hearing r reading the bodhi thing, i definitely knew he didn't know. the jb cut did come from seeds from the original bagseed that spawned the actual chemdog right? didn't skunk va split the seeds that were left with him or some shit? or did i mishear that also lol. i seem to rememeber the D also came from that as well as some other letters that were not good so they didnt keep em.
 

Dr. Who

Well-Known Member
are you culling the rest of the male plant you took the branch off of? or do you have a dedicated seperate room the males stay in all the time?
Short answer: cull

Any project that requires large amounts of seed to be produced is done in another location and in a tent where whole plant(s) are pollinated.
All work with males, are done there.
 

outliergenetix

Well-Known Member
Short answer: cull

Any project that requires large amounts of seed to be produced is done in another location and in a tent where whole plant(s) are pollinated.
All work with males, are done there.
see my plan was to use a separate closet in a separate room to open pollinate my first runs of new seeds I get in a SoG . this way I have allot to preserve for future projects and pass around for ppl who need or want em, but mostly because I felt like it was less work. would you say a separate room is not enough, or it definitely needs to be a seperate location like off site?
ig I feel like the risk of getting pollen on your clothes is similar even your way, although admittedly less of it. ig what I am saying is the same precautions need to be taken going from one "closet to the next, i.e spraying down everything with water and showering self off not wearing same clothes etc... I am def gonna try your way tho. I do want to add my fear doing the seprate bottle to flower the cutting similar to sexing a cut would only give me access to early pollen, idk if it matters but I kinda was hoping for a spectrum of maturity and sizes from the seeds to observe any patterns. like are smaller seeds from more mature plants and later fertilization any diff than fertilization from the first bit of pollen to drop early in maturation. also I feel like it is a bit of an art or skill to pollenate different phenos for the first time because of diff maturity times regarding flowering, and if I SoG open pollinate the entire run with no culling or very very late culling I could avoud that nuance. idk it is allot to think about, but your info will be very helpful
I was also considering male runs in a seperate closet just for males and collecting pollen to use at later dates via single branch like, but I feel like the best way to do that is to clone all the candidates but I vowed to get away from cloning as part of my system. yes I will clone or reveg super special things I find but I definitely don't wanna do what I did before which is clone em all and keep em vegging till I sample the product and then choose the keeper. I am gonna try and reveg something that appears and smells special and then hope taste crries thru if not it's only 1 clone no biggy
btw, if it seems like I thought I was replying to shwaggy I did lol, mb. thanks for answering dr who any feedback welcomed
 

Dr. Who

Well-Known Member
see my plan was to use a separate closet in a separate room to open pollinate my first runs of new seeds I get in a SoG . this way I have allot to preserve for future projects and pass around for ppl who need or want em, but mostly because I felt like it was less work. would you say a separate room is not enough, or it definitely needs to be a seperate location like off site?
ig I feel like the risk of getting pollen on your clothes is similar even your way, although admittedly less of it. ig what I am saying is the same precautions need to be taken going from one "closet to the next, i.e spraying down everything with water and showering self off not wearing same clothes etc... I am def gonna try your way tho. I do want to add my fear doing the seprate bottle to flower the cutting similar to sexing a cut would only give me access to early pollen, idk if it matters but I kinda was hoping for a spectrum of maturity and sizes from the seeds to observe any patterns. like are smaller seeds from more mature plants and later fertilization any diff than fertilization from the first bit of pollen to drop early in maturation. also I feel like it is a bit of an art or skill to pollenate different phenos for the first time because of diff maturity times regarding flowering, and if I SoG open pollinate the entire run with no culling or very very late culling I could avoud that nuance. idk it is allot to think about, but your info will be very helpful
I was also considering male runs in a seperate closet just for males and collecting pollen to use at later dates via single branch like, but I feel like the best way to do that is to clone all the candidates but I vowed to get away from cloning as part of my system. yes I will clone or reveg super special things I find but I definitely don't wanna do what I did before which is clone em all and keep em vegging till I sample the product and then choose the keeper. I am gonna try and reveg something that appears and smells special and then hope taste crries thru if not it's only 1 clone no biggy
btw, if it seems like I thought I was replying to shwaggy I did lol, mb. thanks for answering dr who any feedback welcomed
If you want to use pollen from different age's of dispersal. You will need a safe place to grow males....No going from one to the other with out cleaning yourself - you have a grip on that already.

Collecting and "painting" females. Delivers the best pollen to the best females and you can even pic what you consider the points to hit....

In the end. If your doing a long term project to get specific results...... I suggest at least a 250 seed run. You pheno hunt those and go on from there. This is why quality, stable strains take a long time to go from idea to market...

I'm sure you are reading this whole thread, right? This page is a golden text on male growing and pollen collection for breeding on a comfortable home scale...

Swaggy is rock'in the whole "breeding instructions for the hobby breeder" thing!
So well done and detailed! This is the thread to follow to actually learn how to do quality small scale breeding projects........The real trick is in picking the right strains to cross for your goals....
 
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