mushrooms

jonjon777

Active Member
Can you use the rye seed breeding method to inoculate the flour/vermiculite cake?

As they are two seperate methods, im looking for a way to keep it alive and ability to inoculate when i choose, with ease.

Any info would be awesome!
 

canndo

Well-Known Member
Can you use the rye seed breeding method to inoculate the flour/vermiculite cake?

As they are two seperate methods, im looking for a way to keep it alive and ability to inoculate when i choose, with ease.

Any info would be awesome!

It is extremely difficult to keep mycelium alive for long periods. I believe liquid nitrogen works, some claim that dried mycelium can be brought back to life, and there are certain methods of emersion in sterile oil which is refrigerated.
Of course you can do periodic tissue cloning but sencience will eventually overtake your culture.

Consider it this way, mycelium can only grow to a certain length.
 

madhatter369

New Member
lookin for others who used rye bag with q-tip andwater then fruiting in pasturized manure-box.was 3lb. now5lb.see it asks for 75-85degreese f..seems kinda hot and a bitch to use space heater . so can u use a shroom blancket to wrap or whatever ....help
 

canndo

Well-Known Member
superspawning or substrate to substrate work is dicey in the best circumstances. However, good sterile teqnique when going from sterile substrate to sterile substrate is almost as good as a flow rig.

pasteurized to pasteurized is a crap shoot that I only do in the wild. Wood lovers are a prime example, colonized wood chips can be placed in new banks quite easily.

but if one is serious, they should have a good pressure cooker and a laminar air flow hood. The hood forgives many mistakes.

except one. Exposed sporulating contamination up wind of the sterile work.

that situation will invariably ruin your work, your room possibly your house, perhaps your health.
there are situations where nothing else will do but still air in a glove box.
 
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