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Clean Clones

Discussion in 'Hydroponics / Aeroponics' started by Rahz, Jan 1, 2018.

  1.  
    Twerkle

    Twerkle Well-Known Member

    Hey guys, just wanted to stop by the thread and add my 2c. I love when people talk about live dwc <3333.

    So something that might help you Rahz is alcohol ethoxylates. which is a ester based non-ionic surfacant.

    Basically it stops root rot from spreading between plants in the same hydro system (in this case your cloner). It helps control the spread of zoospores by reducing the effecancy of their flagellum (the little thing that lets em swim). It does not sterilize or disinfect, so it can be ran along side bennies. Algea is a zoospore also so I use this in my DWC as well with every feeding. They also help with nutrient uptake so you can reduce your nutrients by 10-15%. A little goes a LONG way (FOAM!). It breaks down every 3 days or so I add it to my recirc twice a week. You will get an eye for when it needs it by observing the size of the bubbles coming from the air stone.

    Here is a paper for those who want to read it: https://www.apsnet.org/publications...uments/1996Articles/PlantDisease80n04_422.pdf

    Alcohol ethoxylates won't stop your clone from getting root rot but will stop it from spread to healthy clones.

    I use it in conjunction with bennies. I usually run r/o, great white, myco jordan, alchohol ethoxylate, pinch of kelp, and a blend of enzyme powder (cellulase/hemicelluase). I spray once a day with a light fulvic acid to begin to use up any extra nutes left in the leaves to get them searching (leaves yellowing). Ususally healthy clones begin to get nubby in the cloner around day 5 and have roots by day 7-10. I plant as soon as a nice cluster of nubs forms on the stems. Anything not rooted by day 14 is tossed. I change water every 3-5 days. Also i dip in elite 91 rooting liquid after cutting.
     
  2.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    Thanks for the info. Anything that makes life hard for pythium sounds good to me. I'll do some research on alcohol ethoxylates.

    I'm still determined to make it work with rockwool cubes. I have upped my dome count to 3 (I'm going to be germing pepper plants in a month or so) and have a temp controller arriving tomorrow for the heat mats.

    Regarding the infected DWC station, after cleaning it as thoroughly as possible I filled it with water and added some brewed Recharge. Every couple days I added more Recharge changing the water once in this process. I then selected a couple of the cuttings that hadn't rooted yet and placed them in the station, inoculating the net cups with Recharge tea. So we'll see how things turn out. Even though the clones haven't rooted yet I suspect they will develop roots in the net cups. Whether they will get rot again remains to be seen, but I'm hopeful the Recharge treatment has reduced the pythium spore count to low levels.

    I've also added extra bubblers, increased the chiller temp to 72, starting nutrient solution with silica while keeping ppm low and P levels under 20 while vegging, basically using everything I've learned recently to try and give the bennies the edge.
     
  3.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    Clones started Jan-25th - in germination domes at 78 degrees
    Care taken to maintain same stem size and leaf surface for each sample, 24 cuttings total

    3 samples each, 1 rapid rooter and 2 rockwool cubes
    - UC Roots
    - Garden Friendly Fungicide (same strain bacteria as Hydroguard)
    - Azos
    - Root Shield
    - SM-90
    - Daconil
    3 samples rockwool plain water
    3 samples rapid rooters untreated

    Vents will be closed for first couple days.
    Domes will remain over clones for the duration.
    Media will be rehydrated only if necessary.
     
  4.  
    OneHitDone

    OneHitDone Well-Known Member

    Let me throw another product into your equation - Organocide Plant Dr
    http://www.yourplantdoctor.com/plant-doctor-systemic-fungicide/

    This product is solid and I have used it quite a bit on cannabis and especially on tomato's and avocado.
    This brought my outdoor tomato's back from a blight death to looking brand new.
    Amazing stuff

    Did you look at Orca or Companion by Growth Products?
     
  5.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    Organocide is a plant oil based product similar to SM-90, might give it a try. Does it have that fruity smell?

    I almost bought some Great White. Any thoughts on Great White vs Orca? Not real fond of putting clay based powders (not sure if GW is clay based) in hydro... but I don't have any issues using it on rockwool.

    Thanks for the ideas. If every product works similar and I start having trouble separating the good from the bad I'll probably give up on the tests and call it temp/humidity/cleanliness issues. But if one product produces roots faster than others I'll be encouraged to continue testing other products.
     
  6.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    Could be an anomaly but worth mentioning, all three cuttings in the UC Roots sample are wilted. They'll probably perk up but it's the only sample with all cuttings wilted. Several other samples have 1 wilted cutting.
     
  7.  
    OneHitDone

    OneHitDone Well-Known Member

    Plant Dr is "Mono- and di-potassium salts of Phosphorous Acid", not sure if that is derived from plant oil's?
    No odor to it at all from what I can tell - will stick my nose in there next time I open the bottle.

    I have not used Orca yet but it is supposed to be clear and clean. Something I am considering giving a try in my hydro systems.

    The Companion works well in hydro. It looks a bit nasty out of the bottle (molasses base) but at 1ml per gallon seems to clear up after circulating over night.
     
    Rahz likes this.
  8.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    Ahh, my mistake. The Organocide I looked up is "3 in 1 garden spray" and contains sesame oil.
     
  9.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    Updates. It looks like the root rot in the growing station has been eradicated. The type of root rot I had would infect below the water line. I now have roots well into the water, still nice and white. It's possible it could return but I suspect there won't be any rot problems for the duration of the grow so I'll go ahead and post steps and update if anything changes.

    Cleaning an infected system.

    1- scrub/soak all equipment with a chemical agent such as bleach. Titanium lined chillers should be fine with high levels of bleach. Soaking for several days in 2-3% hypochlorite (20,000 - 30,000 ppm) is useful. Water pumps may have issues with strong bleach. While feet and rubber seals can be removed there may be internal parts that degrade. Suggest removing rubber parts and soaking in 1000-2000 ppm solution. Clean rubber with alcohol.

    2- Prepare Recharge (brand of dry Microbe tea). Suggest initial treatment be brewed with warm water for 4-12 hours. This will germinate and invigorate the spores as well as deplete carbohydrate content making it less available to remaining pathogens. I used 1 teaspoon in a gallon, brewed then added to 5 gallons of water in the rez. My system uses more water but because the container was easy to sanitize I didn't see the need to fill the system completely. Enough water should be used to facilitate recirculating through pump/water chiller. Water should be kept at 72-75 degrees.

    3- Insure equipment has been washed of chemical residue and reassemble. Add water and Recharge tea. Turn on pump. Turn on chiller if necessary. On day 3 add another teaspoon of Recharge directly to reservoir. On day 5 the water should be replaced and a new cycle started. This can be extended until new plants are ready. Timing the treatment 5 days prior to the introduction of new plants is ideal.

    4- New plants (netcup/hydrotron/grow cube) should be inoculated with Recharge tea prior to being placed in the system. Initial nutrient solution should be PH balanced to 5.8 and around 4-500 ppm with Phosphorus below 70 ppm. Veg plants don't need a lot of P so it wouldn't be an issue to run as low as 20 ppm P.

    5- Maintain this microbe friendly nutrient solution for 1-2 weeks. Beyond this it should be okay to resume a typical nutrient schedule with or without the addition of microbes.

    Clone update: Day 8 no roots showing yet. The prior set of plants was transferred to a soil-less media and all of them have survived and are growing. Current set is still under domes. I have 3 heat mats connected to a single temp controller. I have been monitoring temps in all three domes. Variances of around 2 degrees Fahrenheit (76.5-78.5) have been observed. This seems acceptable to me. Hopefully we'll see some roots in the next couple days.
     
    Last edited: Feb 2, 2018
    OneHitDone and Sour Wreck like this.
  10.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    14 days, cuttings are healthy and no roots yet. I'm not sure what to do at this point to speed up the rooting process. I know that I can take unrooted cuttings and place them either in net pots or in soilless media and they will root so it's not essential that I get to a point where I have pretty roots poking out of cubes in 7-10 days, but it would be nice.

    The main thing is avoiding root rot. I think I can accomplish that with any of the products I've been using including plain water. One thing I've learned about Pythium is that it doesn't have an airborne phase. It can get kicked up in dust but that possibility can be minimized by keeping the floors clean. Using sanitized equipment and grow media, washing hands prior to handling anything, all part of the practice to avoid unwanted microbes. Keeping the germination area well above the floor also makes sense.
     
  11.  
    OneHitDone

    OneHitDone Well-Known Member

    Can't seem to find in the thread - are you using any form of rooting hormone?
     
    Rahz likes this.
  12.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    I'm using a cloning gel called Rootech.
     
  13.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    One thing I've noticed, the cuttings in the rapid rooters look much better than the rockwool. I let things get a little dry and I'm sure that's the issue. So rockwool dries out much faster than rapid rooters. Whether this is good or bad depends. Having to hydrate the rockwool more often means more fresh air getting to the stem.
     
  14.  
    zypheruk

    zypheruk Well-Known Member

    If they are still healthy in a few weeks in their final plant site, that will be the real result.
    I gave up on rdwc because every fucking time after a few weeks bang rot Tried every product know to man, and the only thing it done was empty my bank account. A few years back I was growing in bubblers with no problems, was great. Water company are installing new pipes all over the country here atm so may well have something to do with it. So I'm back in coco for now. But will eventually go back to flood and drain table, rather liked it and simple to run. Have loads a kit to play with now lol.
     
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  15.  
    Sour Wreck

    Sour Wreck Well-Known Member

    fingers crossed, but i currently have my root rot beat.

    took nothing less than a dozen bottles H202, pool shock chlorine, putting clean clones in dirt for a while and new tubs.

    i got clean, long white roots again.

    f me !!!!

    don't want that shit again.

    [​IMG]
     
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  16.  
    Rahz

    Rahz Well-Known Member Rollitup Advertiser

    Roots! Day 23 and there are roots on a handful of the cuttings.

    Without exception the cuttings put in rapid rooters are the ones with roots showing. I did let things get a bit dry at one point but not to the point they were wilting so I don't know if the rapid rooters are better than rockwool or just more resilient to neglect. Either way, I'm switching over to rapid rooters only.

    Most developed roots are in the Azos tray. They are 1/2 to 3/4 inches while the other roots are just poking through.

    Very happy to finally see some action.

    Something else I want to do but haven't yet is use Recharge in some of these rooters. The directions suggest applying it after the cuttings have rooted but I think it might be okay to use earlier, like prior to putting the cuttings in them. Great White is a similar product which is listed for cuttings. The difference is, Recharge has kelp and molasses. It's possible the microbes will become to vigorous and damage the stems... we'll find out. It's also possible airborne pathogens could land on the rooter and use the molasses to gain a foothold. I'd also like to get some Great White and give it a shot.

    The Hydroguard seems like a good product, but I'm going to try and start working with powders as much as possible. Powdered microbes are going to have a much better shelf life, be less prone to temperature variables etc. Live liquid cultures loose vigor 6 months at refrigeration temperatures. Spore based liquid cultures should last longer, but should probably still be refrigerated. I read about a test someone did and discovered various liquid based biologicals were either deficient or devoid of microbes. The main issue may be storage time prior to purchase.

    I'll start a new test soon and provide data as it's available. Something else I'm going to play around with is to dilute the cloning gel into a bit of water, enough for it to have a liquid consistency, and use a syringe to apply it into the rooters.
     
    OneHitDone likes this.
  17.  
    OneHitDone

    OneHitDone Well-Known Member

    Hey @Rahz , hope you got the cloning all sorted out...

    Rapid Rooters may or may not be great - WAY too expensive per plug so I've never mad an effort to implement them.

    I like Oasis but keep coming back to the 1.5" Rockwook cubes. They are just easier to source, root well, and are affordable.
    Soak plugs in light nutrient ph'd solution, dip in Clonex (or your choice of hormone), and stick.
    Been like 99.9% success for me for the past several years.
    Just mist daily with straight water till roots are popping.
    I use 2 Wally's tote's of the same size with one upside down for a dome slid over slightly for air flow.
    Unless your running a commercial op you probably won't need full 10x20 trays of clones every batch.
    Note my Grodan Grow Smart Tray or whatever it's called cut down to fit my smaller totes :hump:

    IMG_2098.jpg
     

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