a mediocre shroom garden thread

[Wolfedawwg]

I've been slack as fuck lately on the mushroom side of things.......been busy with other stuff...
I've got about 8 ghetto petri dishes (AKA pasty plates) of cultures that I cloned from my lats 2 tubs that pretty much all fruited....
I'm gonna make a point to transfer a few of these today and get my arse back in gear.

 

[iHearAll]

I've been slack as fuck lately on the mushroom side of things.......been busy with other stuff...
I've got about 8 ghetto petri dishes (AKA pasty plates) of cultures that I cloned from my lats 2 tubs that pretty much all fruited....
I'm gonna make a point to transfer a few of these today and get my arse back in gear.
View attachment 3942939

lol do you just mix the agar on the stove and then microwave it all in the tupperware? or is that a different based petri dish?

 

[Wolfedawwg]

lol do you just mix the agar on the stove and then microwave it all in the tupperware? or is that a different based petri dish?

I mix it up on the stove, then into the pp5 conatiners, then PC about 30-45 mins.

 

[Wolfedawwg]

The material has to be pp5 plastic with a small hole drilled in each lid with a couple layers of micropore tape or they will likely implode.
I have heard talk of microwaving it but for the sake of 1/2 hour or so, I feel safer using the PC.

 

[iHearAll]

The material has to be pp5 plastic with a small hole drilled in each lid with a couple layers of micropore tape or they will likely implode.
I have heard talk of microwaving it but for the sake of 1/2 hour or so, I feel safer using the PC.

ah, yea i wouldnt have thought any polypropylene was pc safe. i know polystyrene for sure isn't!

 

[Wolfedawwg]

ah, yea i wouldnt have thought any polypropylene was pc safe. i know polystyrene for sure isn't!

Yea pp5 is fine (glad mini-rounds work great), you just have to be careful the sides of the container don't touch the sides of the PC.

 

[iHearAll]

1/2 pint LC lids are done and each has a stir bar inside

I sterilized 5 jars of 5% honey solution to work on monocultures for better flushes. I also am extending my pan goliath jar here. The syringe was a clump of spores and wouldnt separate. So, i injected the clump into one jar of coffee and grass. I still tried using the rest of the syringe in the other jars i had prepped but they havent shown any signs of having been inoculated. I guess the appropriate thing to do is a transfer when i go to birth this sucker.


just a pic of the gaggle of mixed ages and strains.


Also, the agar dishes dont seem to be doing anything. I'm not sure there were any spores in the syringes i used lol

 

[calliandra]

Ah I always know I'm going to dive into another world when I come here
MAaan, you're genius, and when I think that you had to source those parts first, which to me is the worst pita on the whole wide world, I'm all in awe

 

[iHearAll]

Ah I always know I'm going to dive into another world when I come here
MAaan, you're genius, and when I think that you had to source those parts first, which to me is the worst pita on the whole wide world, I'm all in awe

Thanks cali, I have the same feeling when I attend your gardening thread I love your microbiology information so much!

 

[iHearAll]

biopsy specimen. I sucked up a few syringes full of boiling water and once they cooled with the water still inside, I wiped down the outside of the specimen's stem with iso, poked the syringe through the stem and pushed the contents of the needle tip with a little bit of the buffer water from the syringe into the sterile LC jar. I took two samples of this cap into the same jar. The mycelium at the center of the stem is hopefully now the mother culture of this and everything later produced with it if all goes to plan . The jar's self healing port (blue silicon thingy) worked like a charm and the syringe filter (green thingy that is normally a micropore filter for syringes) allowed enough gas exchange for the process to go very smooth. We'll see by next week if i cleaned my sample well enough.

 

[calliandra]

biopsy specimen. I sucked up a few syringes full of boiling water and once they cooled with the water still inside, I wiped down the outside of the specimen's stem with iso, poked the syringe through the stem and pushed the contents of the needle tip with a little bit of the buffer water from the syringe into the sterile LC jar. I took two samples of this cap into the same jar. The mycelium at the center of the stem is hopefully now the mother culture of this and everything later produced with it if all goes to plan . The jar's self healing port (blue silicon thingy) worked like a charm and the syringe filter (green thingy that is normally a micropore filter for syringes) allowed enough gas exchange for the process to go very smooth. We'll see by next week if i cleaned my sample well enough.View attachment 3946702

Ok I didn't get that lol
you pushed the water through the stem, which took some mycelium with it, that is now innoculating the jar contents?!?! But no, that doesn't work, how is that ever going through that syringe filter thingie...

Great that everything worked to your satisfaction though!

 

[iHearAll]

Ok I didn't get that lol
you pushed the water through the stem, which took some mycelium with it, that is now innoculating the jar contents?!?! But no, that doesn't work, how is that ever going through that syringe filter thingie...

Great that everything worked to your satisfaction though!

Not quite how its working here the green syringe filter is not passing liquids in its current use. It merely to act as a permanent air filter to promote clean gas exchange. Other things work too, like a bandaide, or micropore tape. But, the plus side of the syringe filter is i can put the entire thing in a pressure cooker.

The blue silicone button will heal itself when punctured. I don't push water through the stem, I actually just catch a circular sample the size of the syringe hole and use the water in the syringe to push it into the jar through the blue silicone port. The tiny amount of mycelium in it should grow into a glob over the next ten days

 

[farmerfischer]

OP! ( to noobs)When picking wild shrooms never go by bruising. In my state many shrooms bruise blue, purple, and blue green and are not active and some will make u sick or even kill you. And example would be bruising bollets . they bruise and are not active. Spore printing and viewing is the only way to correctly I'd shrooms.I have pics some where around here of the bruising bollets

 

[iHearAll]

OP! ( to noobs)When picking wild shrooms never go by bruising. In my state many shrooms bruise blue, purple, and blue green and are not active and some will make u sick or even kill you. And example would be bruising bollets . they bruise and are not active. Spore printing and viewing is the only way to correctly I'd shrooms.I have pics some where around here of the bruising bollets

Oh yea definitely! I think I recommended he/she tale a print to prove it is what they think it is. Maybe a different thread....Hmm..yea I have eaten falsely identified shrooms and was sick for over 24 hours of pain and vomiting and diarrhea .... Exciting

 

[farmerfischer]

Oh yea definitely! I think I recommended he/she tale a print to prove it is what they think it is. Maybe a different thread....Hmm..yea I have eaten falsely identified shrooms and was sick for over 24 hours of pain and vomiting and diarrhea .... Exciting

Yeah you told OP to spore print for id. I was stating the bruising isn't a way to tell if they are active. To many times I've hear or read people telling others" if they bruise or stain" there good to go.. Just wanted to let noobs know that is wrong!!. I look for shrooms every year, Actives and edibles, even if I'm certain what they are I always print.
Years ago like a dumb ass I ate what I thought was liberty caps from an old cow pasture and they were not..lol same thing, stumic cramps major diarrhea and vomiting. Sweating like crazy. Fucking learned a lesson that day and counted my lucky stars I didn't kill myself.

 

[iHearAll]

Yeah you told OP to spore print for id. I was stating the bruising isn't a way to tell if they are active. To many times I've hear or read people telling others" if they bruise or stain" there good to go.. Just wanted to let noobs know that is wrong!!. I look for shrooms every year, Actives and edibles, even if I'm certain what they are I always print.
Years ago like a dumb ass I ate what I thought was liberty caps from an old cow pasture and they were not..lol same thing, stumic cramps major diarrhea and vomiting. Sweating like crazy. Fucking learned a lesson that day and counted my lucky stars I didn't kill myself.

Ouch sorry you had to experience that.im sure you're bettrr from it lol! Fuck.
Yea some cultivation websites really don't like members discussing identification because new members may try and answer it and give wrong advice while Joe shit, the kid, trying to get high immediately eats them after this false identification...

 

[calliandra]

Not quite how its working here the green syringe filter is not passing liquids in its current use. It merely to act as a permanent air filter to promote clean gas exchange. Other things work too, like a bandaide, or micropore tape. But, the plus side of the syringe filter is i can put the entire thing in a pressure cooker.

The blue silicone button will heal itself when punctured. I don't push water through the stem, I actually just catch a circular sample the size of the syringe hole and use the water in the syringe to push it into the jar through the blue silicone port. The tiny amount of mycelium in it should grow into a glob over the next ten days

Aaaaah ok thanks for clarifying
All the more amazing, so it's just a speck that does it!!!

 

[iHearAll]

Aaaaah ok thanks for clarifying
All the more amazing, so it's just a speck that does it!!!

 

[HeatlessBBQ]

Dang... I wish I knew You... Its been a minute since I have eaten mushrooms.
Pretty much a necessity at this point.

I cannot gorw mushrooms anymore... I have spores and equipment to grow
but I am under surveillance, so it is virtually impossible for Me to grow mushrooms ever again ...
unless they become legalized.

 

[GroDank101]

My jars are almost done. What do y'all think, about another week for me until they're consolidated?