Papain Adulteration in 11-nor-Δ9-Tetrahydrocannabinol- 9-carboxylic Acid-Positive Urine Samples

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Papain Adulteration in 11-nor-Δ9-Tetrahydrocannabinol- 9-carboxylic

Acid-Positive Urine Samples

Authors: Larson, Scott J.1; Holler, Justin M.1; Magluilo Jr., Joseph1; Dunkley,

Christopher S.1; Jacobs, Aaron2

Source: Journal of Analytical Toxicology, Volume 32, Number 6, July/August

2008 , pp. 438-443(6)

Publisher: Preston Publications

The adulteration of urine samples is an ongoing problem in forensic drug-testing laboratories, even in the military where the practice of observed collections is performed. These adulterants are used to produce a false-negative result when samples are analyzed for drugs of abuse. It has been reported that papain, a cysteine protease, could be successfully used as a urine adulterant, altering the concentration of 11-nor-Δ9-tetrahydrocannabinol-9- carboxylic acid (THCCOOH) in urine samples. The current study analyzes the effects of latex papain (Sigma, 10 mg/mL) and Lawry's® Adolph's Meat Tenderizer (papain is an active ingredient, 10 mg/mL) on immunoassays (FPIA, EMIT, KIMS) and gas chromatography-mass spectrometry (GC-MS) analysis for biological samples.

The samples were analyzed initially between 2 and 4 h and then at 1-, 3-, 7-, and 10-day time intervals after the addition of papain. A decrease in response averaged over the course of the study was observed with FPIA (Abbott, 22%) and EMIT (Syva® Dade Behring, 26%, Microgenics, 10%) screening assays by the addition of latex papain to the samples. An increase in response was found using the KIMS (Roche) assay (156% increase). In addition, the GC-MS results (27% decrease) demonstrate that papain affects both the screening and confirmation assays. The addition of meat tenderizer caused decrease in the FPIA (Abbott, 11%) screening assay and GC-MS results (22%) similar to the latex papain while having varied results on the other screening assays. This study confirms papain could be a potential problem for urine drug-testing programs.

All the enzymes should be tested, the modern piss tests are a vicious invasion of privacy.

I failed one of the modern wonders because I have an elevated WBC. One time in 39 years no thc and I failed. I demanded a mass spec. They sent me a letter false positive on the preliminary test called an ISA or similar.

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