Growing Mushrooms....or trying at least.

Mr.KushMan · #1 02-03-2009, 11:02 AM

About 2 months ago

hemlockstones · #2 02-03-2009, 11:46 AM

"When I looked at the part of the syringe that holds the water I noticed that there was only a chunk of mycelium inside the syringe, and there wasn't much in each one."
shouldnt be mycelium... just spores.. dark dots or clumps. wouldnt be too many of them, you might not even see them. if you do see a clump just shake the syringe. it would actually be illegal to send Cube mycelium in the mail.

"So I ended up only inoculating 4 1pint narrow mouth jars with each of my 2 syringes"
so you used 2 syringes on 4 jars!? the syringes 101 sends are 10-12cc... and you only need one CC per jar... 2 syringes is enough for 20-24 jars....
if you put too much in the jar it throws off the moisture content and you get contams.

a decent print 3-5" can make like.. 20 syringes...
more spores isnt alays a good thing, only the genetics from one spore will win over the substrate anyway.

if its just the yellow piss then it isnt anything bad, just wash it off when you fruit.
if its BRIGHT yellow, and its growing then its bacteria...that is bad

anything contaminated id tell you to throw away, bleech the jars, rinse and start over.
if this is your first grow, go with BRF/PF tek, casing birdseeds opens you up to more contamination.

if your getting over 50% contamination rethink your mix, sterilization and inoculation procedures.
aim for less than 20-10% contam

good luck

Thompson3600 · #3 02-03-2009, 04:27 PM

Quote:

Originally Posted by hemlockstones

anything contaminated id tell you to throw away, bleech the jars, rinse and start over.
if this is your first grow, go with BRF/PF tek, casing birdseeds opens you up to more contamination.

No way. Bacteria has long before known to be beneficial to mushrooms as they immediately start fruiting and WILL still grow for survival of the species. Bacteria in a bulk substrate is not a problem, bacteria on fruits are a problem and are discarded....however they can still be eaten if 100% cracker dried.

Thompson3600 · #4 02-03-2009, 04:48 PM

Quote:

Originally Posted by Mr.KushMan

About 2 months ago I ordered a few syringes from spores 101.com When I got them I immediately started preparing my WBS cakes. Pressure cooked them and left them over night. The next morning when I woke up and looked at my mail I had received my spore prints from FSRE.com. When I saw they sent me 1 B+ spint and 3 GT prints I was ecstatic, but it took over a month for me to receive them. So after a while I decided to open my syringe packages and start inoculating. When I looked at the part of the syringe that holds the water I noticed that there was only a chunk of mycelium inside the syringe, and there wasn't much in each one. So I ended up only inoculating 4 1pint narrow mouth jars with each of my 2 syringes. So I was a little upset but I figured when I do my spore sprint syringe I will have better luck. So about 2 days later while the first batch was inoculating I decided to get a few more jars going so I got 8 1/2 pint jars PCed and ready to go. The next day I used boiling water and drew it up the syringe and relieved it in the sink, then drew up some more water, ect. When I got the syringe full of water after 5 or 6 cycles, I let it cool for a few hours. Then I prepared my spore print. I used a whole print for 1 syringe. Then I inoculated up my 1/2 pints. The amount of spores was so prolific that there was actually spore residue on the bottom of the jars(dark black speckles). I figured that these would just fly. So next I left them in the incubator with the others. By this time half of the 1 pints had a little almost moisture like looking growth about halfway up the sides and bottom of the jar. I then waited about 4 more days. Each day the 1 pints growths got about 5/4s the size of the previous day. The something weird happened. The next day I woke up and the 1/2 pint jars were at like 60%, then the next day they were at 75%. So I shook them up. The next day they seemed to slow down again, and by day 10 they were all at about 95%. But when the 1/2 pints took off, the 1 pints just stopped at probably 3%, and some were visibly at 0%. So I shook them all, I figured I would kill these less colonized ones if I did, but I did anyways. So after a while the bigger jars started to get a black tinge to the seeds. We have a terrible black mold problem. But my white jars continued to truck on. Then I showed my brother one jar and all of the jars had like a piss yellow colored liquid at the bottoms, every jars except 2 1/2 pints. So at that point I removed all the contaminated jars. Then a couple days later I decide to birth them. I opened the first and saw a green patch on the top middle, I put the lid back on so quickly, hahaha. And set it aside, then I poened the other and it was still good, so I broke it up put it on a penut butter lid and back in the incubator. So I was just wondering what I should do from here? Should I put in a conatiner and make somekind of casing? or just friut as is? or can I add it to more WBS? Oh and if someone could tell me why the balck mold did not attack any of my jars, but trich got one and the piss got a few?

Peace Thanks in advance.

1. WOA(Word Of Advice), don't order from spores101 for a number of reasons, about a month ago I've heard of 3 people getting contaminated or syringes that wouldn't even grow anything at all. If you want reliable sources order only from www.shroomery.org/sponsors ... Hawks Eye and Sporeworks are nice and reliable. Ralphstersspores is 10x better and has a lot more patience for noobs and if sent a bad or syringe didn't get there, he'll replace it in the quickness. I'm not trying to diss spores101 but lately they've been getting a bad name.

2. Remember, FSRE are a free spore doner which get prints from anyone to send them to those in need. They can be dirty or clean, it doesn't matter and most of the time, they're noobs who don't take the nescessary percausions to keep them clean, REGARDLESS you should already know agar and be isolating.

3. What temperature were you incubating at? How were you providing gas exchange? What was your incubator setup(describe)? What does it smell like(the mycelium)? It should have a fresh mushroom smell.

"I've been trying to correct that disinformation for years. It's all based on a chart somebody mailed to stamets many years ago showing 86F to be the peak temperature for growth of cubensis on a petri dish, and everybody just accepts it as though Moses carried it down from the mountain on a tablet of stone. However, every single experiment I did to try to duplicate that with extremely accurate temperature monitoring was unable to verify that bogus 86F figure. What I have repeatedly found regardless of strain is that cubensis colonization remains rather flat from about 75F through 81F. Beginning at 83F, the rate of growth falls off sharply. By 86F, growth has slowed down nearly 50% what it was between 75f and 81F. These experiments were conducted on petri dishes that produce little to no heat because of the very thin layer of mycelium. In jars, up to several degrees of heat is produced by the colonizing mycelium; so definitely don't go over 80F to 81F if you're looking for maximum rate of growth. Furthermore, bacteria and thermophilic molds such as Mucor, the black pin mold are stimulated by higher temperatures. Therefore using an incubator set to 86F is certainly favoring bacteria and molds, while slowing down mushroom mycelium growth. Below is a picture of one of my colonization shelves. It sits in my bedroom at normal room temperature and quart jars of rye berries colonize fully in ten days, and pf jars colonize fully in 14 to 21 days, but usually closer to 14. How often do we see posts where people have incubators set at 86F, and they're asking why their jars aren't colonized after four to five weeks, and they have large spots of yellow liquid forming? The liquid is metabolites that the mycelium secretes in response to stress, usually from competitor molds and/or bacteria. What has happened, is they've slowed down the mycelium while stimulating the competitors. COLONIZATION"

4. The Mycelium Piss is called METABOLITES(it's caused by stress by high temperatures, bacterial contamination). What's happened is that the competitors(mold/contamination) has slowed down the mycelium and trying to grow there own but the mycelium is like fuck you and excreting metabolites to try to kill it which it can kill trichoderma on site.

5. If I were you I'd cut out pieces of the fully colonized cake and add it to more grain jars(something called G2G transfers), use a still air box, if you don't know how to build one there's good 'teks' on shroomery on how to make them, WOA: FUCK THE POSITIVE PRESSURE and FAN bullshit. It's called a still air box for a reason. Thermophilic molds/contamination are stimulated at temperatures beyond 80F, which is why I am against incubators in the first place. They'll colonize fine in 6-8 days if you play your cards right at 78F. Room temperature 70-78F in ambient room lighting is fine.

hemlockstones · #5 02-03-2009, 07:50 PM

Quote:

Originally Posted by Thompson3600

No way. Bacteria has long before known to be beneficial to mushrooms as they immediately start fruiting and WILL still grow for survival of the species. Bacteria in a bulk substrate is not a problem, bacteria on fruits are a problem and are discarded....however they can still be eaten if 100% cracker dried.

ok this will be my last post since your in a hurry to get in a pissing contest and rack up posts apparently...

the cakes dont piss to kill bacteria!? i thaught you said mushrooms love bacteria hahahaha

saying "bacteria is beneficial to mushrooms" is ignorant. esp to someone whos having contam issues.
what youre talking about is pasturizing bulk substrate.
we get that you can copy and paste, but that is retarded

...you go ahead and eat mushrooms with bacteria growing on them if you want.

ill leave you the rest of the post to copy and paste some more stuff

Thompson3600 · #6 02-03-2009, 07:55 PM

Quote:

Originally Posted by hemlockstones

ok this will be my last post since your in a hurry to get in a pissing contest and rack up posts apparently...

the cakes dont piss to kill trich? thats just made up.

saying "bacteria is beneficial to mushrooms" is ignorant. esp to someone whos having contam issues.
what youre talking about is pasturizing bulk substrate.
we get that you can copy and paste, but that is retarded

...you go ahead and eat mushrooms with bacteria growing on them if you want.

ill leave you the rest of the post to copy and paste some more stuff

Just trying to dismiss misinformation

hemlockstones · #7 02-03-2009, 07:57 PM

what i figured hahah

Thompson3600 · #8 02-03-2009, 08:37 PM

Quote:

Originally Posted by hemlockstones

ok this will be my last post since your in a hurry to get in a pissing contest and rack up posts apparently...

the cakes dont piss to kill bacteria!? i thaught you said mushrooms love bacteria hahahaha

saying "bacteria is beneficial to mushrooms" is ignorant. esp to someone whos having contam issues.
what youre talking about is pasturizing bulk substrate.
we get that you can copy and paste, but that is retarded

...you go ahead and eat mushrooms with bacteria growing on them if you want.

ill leave you the rest of the post to copy and paste some more stuff

I'm not trying to rack up posts at all.
I never said they love, it's beneficial...
Reason being they throw out bacteria blotch on the mushrooms themselves at mushroom farms is people will eat them fresh/dry. They don't know and it would be kind of silly to on that package right, "Dry before consume" when people are expecting to eat them cooked. Dry it cracker dry, it's fine.

weedman11 · #9 02-04-2009, 10:08 AM

If you are having a problem with contamination its most likely your sterilization and inoculation steps. The easiest way to get around this, or the almost fool proof way is to buy pre-sterilized substrate jars.

http://visiongrowkits.com/prod/jars.htm

This is the website i used when i started on my first adventure with mushrooms.

Ive used these many times to inoculate my bulk substrate after the jars were colonized.

Hope i can help

KP2 · #10 02-04-2009, 10:16 AM

i've tried wbs, and i don't like it. too slow, too fussy. i'm using cracked corn now, with awesome success, colonized jars in 6-10 days, and no water/contam issues.

i've thrown out 36 jars of birdseed to date. all the corn but one jar has been successful, and my first corn casing is fruiting now

.

corn rox, but don't follow the tek. soak for 24 hours, wash really really well, let it drain for an hour, then jar and pc for 90 minutes.

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