Marijuana Tissue Culture Success!

[Gastanker]

So no pictures?

I purchase lab cultured hemianthus callitichoides for my aquarium but lab cultured pot plants just sounds like silliness. Perhaps a good way to illicitly transport clone only strains but a bit of a joke when it comes to everything else. I know several people experimenting with cannabis cultures and although it is possible it just isn't practical. If you do manage to have a culture both root and create leaves without molding the likelihood of successfully transferring the plant from a sterile growth medium to a regular pot is damn near zero. When successful the plants are extremely weird looking and take months and months to develop to the point that you can take a successful clone. Yes it is possible but seeds > tissue cultures on way too many levels to list.

And yes, proof matters when making crazy claims. No pictures = no proof makes a ton of sense to me.

Especially with shit like this:

You can easily have roses with thc, or mj without leaves, or even glow in the dark, really, I've seen it !
​

Picture of glow in the dark MJ please.

 

[Girdweed]

Picture of glow in the dark MJ please.

I'll second. You don't need macro for glow in the dark marijuana.

Optimistically, you have it nailed. Honestly, you come across as a scam artist. Definately do some work with a PR group if your wild claims are factually based.

 

[pharmacoping]

Once again for the illiterate among us..I DO NOT MANIPULATE THIS WAY. I HAVE NEVER MADE A GLOW IN THE DARK MARIJUANA PLANT. I have seen this though, done with using the dna of a jelly fish that illuminates.You can buy these genes for manipulation. They are available, as well as hundreds of amino acids to change/manage/cause genetic changes in your mix, they are available,and many are already patented, for marijuana. I didnt invent this. I do not have photos of it. you do not have to believe it either. I am here to talk to professional cultivators about cutting edge growing science, not to convince you that genetic manipulation is real, and real old news. I do this, have done this, will continue to do this in my garden. I have also learned hundreds of different mixes to not use, hundreds of ratios that dont work, vegging techniques that dont work, and hardening off techniques that dont work. I use four hormones, and a preservative. If sterile technique is used, there is no reason to have "mold" in or on your plant. I lose 10% of my explants within the first five days,as bleaching/or otherwise sterilizing the pieces is a critical time for the sample. after that I have never seen mold in a dish, or a vegging plant from a dish. My cuts are bleached, for 15-20 minutes, stirred with a machine, then sterile rinsed,agitated, then rinsed in 70%alcohol for a few minutes,sterile water then transferred to a sterile home,under sterile positive hepa air conditions, that stays sterile. whenever I've failed, I keep trying until I've reached my goal, to bad for your buddies, they were so close..

i use 30-50 jars/tubes. one makes 4 more jars worth in 3 weeks, then they do the same, and so on, adding to as many as desired. We have no idea what hormone/nutrient mixes/sterile techniques etc your culturing friends used, but we do know they are not sterile, each and every time they transfered, I wonder why? because these molds and bacteria will affect your plant in adverse ways, and most likely kill the weak ones. If they make it that far, hell, they'll even look funny and take forever to establish, making one think again, about your friends' techniques. But at least they admit, and embrace the new technology, and commit to trying it out for themselves. A+ to them warriors. my plants do not look much different that yours my friend.



for you serious ones though, maybe keep using private messages. it's great to hear from you and hope I can help.
I know it's risky here, but if anyone else has done this, you dont have to show your face, but please pm me?

 

[Gastanker]

Yeah... the guys I knew were using a sterile university lab... But you are saying you have 90% success in a home made lab? And you are suggesting others can have the same 90% success using a home made lab? Or do you suggest most growers invest big bucks on a professional grade lab? I see pictures of cloned/seed grown plants and bud, where are the pictures of the developing tissue cultures? If you have personally seen glow in the dark MJ why can't you get pictures?

Mind posting a whole grow?

 

[pharmacoping]

imagining my pictures will be stolen or something, right? not sure how things can be proved. I'm not a filmographer, I do not have thousands of shots to show off at a internet forum, I dont have film, elapsed or otherwise, I'm not on ebay. no wild claims here, except this beats the plant count, thats all. successfully . and that I am not the only one doing this, even amongst your readership. backoff with the proof thing, I cant win. no scam artist here, not wanting money, or a prize, only to meet others, that make these miracles happen within their home. I am immediately made aware of the reason they overlook this forum when considering sharing with others, or learning more themselves. I sure hope my photo skills suffice, albeit not proof of anything really. all pics you see are simple tissue culture, in a few different stages, even the end results, which are not much different the the great weed you all grow, but I got my max 72 in flower and veg, and none in clone, except monthly, for a couple days. it works well for me, and a few fortunate persistant people, it does not have to work for you, nor do you have to give it a second thought, but when you do hear about it, send me a posi vibe please. I do have garden pics too, and even some stellar photos of Riot seeds in flower, as promised some time ago. if anyone cares, pm me. all pics at this thread, thusfar, were taken today or recently, and are all from a jar or test tube, originally grown from callus. whats it gonna take?

 

[connoisseurde420]

Im with you ^^^ herd about this years ago anyone with netflixs should know about this big breeders are already on it!

 

[Gastanker]

By all means tissue cultures are possible but very very few people have success with cannabis.

You are making wild claims and you are trying to sell things - reread your first post; you both state you make kits for sale and go on to say you have seen glow in the dark cannabis as well as THC laden roses. Those are WILD claims. To say that you can go from a tissue culture to a rooted regular plant in days is also a WILD claim.

I would love to hear more about your method but please post about your method with substantial photo documentation to back it up - not wild claims and begging for pms (this sounds like you are trying to sell something). I would love to see a day by day progression from tissue culture to plant. You have 70 on their way? Please show us these photos! But please don't just post 70 plants as those of us who understand the process of tissue culture know that they look drastically different than a regular plant. Absolutely no need to show bud shots as they are really inconsequential.

I do this very well, and assemble kits for sale You can easily have roses with thc, or mj without leaves, or even glow in the dark, really, I've seen it ! It's not the future, it's now !
I'll answer some q's here, but private message me for instructions for above.
​

 

[pharmacoping]

let me rephrase, I have seen hundreds of clones/plants come from a dish, but never seen mold on or around them. by the time they are this mature, they seem much more resistant, even able to harden off with the lid off.

gastanker,

I do often get 90% success, during the first transfer of material, but typically it is 100% thereafter. I am not suggesting that anybody can do this, as we know by visiting the newbie forums, some cannot even cultivate, let alone market/plan a grow. this is what i do. i came to meet others that do it. I have made starter kits, with instructions for people, for money, thought I'd cover that . I recalled that offer in a previous post, after some pm's with requests, for my own reasons. I will share links that I come across though. I'm non combative, and really not into the whole "defend every post" game, as evidenced in many many areas in this forum. I really want to meet some class act cultivars, email chat only. but this might do it eventually.
also, again, each pic was started in a dish, regardless of your fears, or misinformation, or even experiments you may be privy to. growers, most wold not invest in an expensive lab, thats silly to suggest, but on par with the peer majority. I've explained how, and where I do this. it is not expensive. the tent was less than 500$pressure cooker80. no secrets here, i saw the same results you did, alot, but not now, thats all, for years. most commercial foods produced by monsanto seed corp are done this way, its not voodoo, dont be scared. these same people are the ones that are responsible for demonizing this plant in the first place. it worked too. "they're not so big you know, you're just on your knees." dont spend your money unless you are sure you can do it.........if you think you can do it, or not...either way, you are right !




#1 is callus, dozens of callus, made to root immediatly are cut from this every few days, or as needed to refill the rooting jars, which are refilling the sprouting jars. which are refilling the veg trays, which refill the flowering trays. these stages are shown in the same pic, old cam, but visible. rooting area is light sensitive, so pics will be sporatic. veg pics comin soon

I sure wish I had the skills to recognize a plants origins by its stature, whether cloned or manufactured, or??? I try, but all the banana tree look the same to me, grown in a test tube, or the dirt. in FL, or online is proof.you can buy tissue culture bana trees, orchids, venus fly traps, some are ony sold this way. some cannot be shiipped unless in a sterile medium sealed vessel.

nitenite, some of us work all day, not sit in class. but i ove ya man, you'll see soon enough whats going on
http://buymarijuanaseeds.com/community/threads/glow-in-the-dark-marijuana.16304/

http://www.washingtonpost.com/blogs/blogpost/post/glow-in-the-dark-cats-jellyfish-and-monkeys-may-prevent-aids/2011/09/12/gIQAdq89MK_blog.html
http://blogs.scientificamerican.com/observations/2011/09/12/jellyfish-genes-make-glow-in-the-dark-cats/
http://www.time.com/time/magazine/article/0,9171,962873,00.html

now, can we move forward. of course the reporters did not talk with anyone but the large labs. but this should put most at ease. we can only hope

 

[Gastanker]

You've convinced me! Major labs made glow in the dark cats so they much be working on marijuana and I'm sure you were there to witness it! I want a kit! How much! How much!

Seriously, how much are you charging for your tissue culture kits? I know of several competitors - can you beat them?

 

[PixiDustr]

I grew rootless tobacco plants from such tissue cultures in Erlenmeyer flasks in the late 70s when I was in college. Least differentiated form looked like apple sauce, the next form looked like corn pops and finally the actual plant. All were grown in different agar solutions based on the type needed. I grew thousands for radioactive electrophoresis experiments for biochemist Professor Chong Maw Chen at the University of Wisconsin-Parkside. It would be easy to have such lab at home. Just need an autoclave and UV-lighting booth for transfers...and there was ALWAYS some contamination. I used to keep a zoo of different infections that occurred. The coolest looked like a putting green with little stalks that had red balls on top. There is no reason the same couldn't be done with marijuana.

Yeah... the guys I knew were using a sterile university lab... But you are saying you have 90% success in a home made lab? And you are suggesting others can have the same 90% success using a home made lab? Or do you suggest most growers invest big bucks on a professional grade lab? I see pictures of cloned/seed grown plants and bud, where are the pictures of the developing tissue cultures? If you have personally seen glow in the dark MJ why can't you get pictures?

Mind posting a whole grow?

 

[pharmacoping]

hortipharm is doing very much, and even patenting marijuana genetic mutations right now, created in the same manner. suicide gene-done-thc-cbd ratios-done look into it..you'll see


re read the thread, I immediately retracted the offer to assemble or sell anything. sorry. I really just tried to cover all the shit first, for the nay sayers and kids to ponder, while trying to connect with advanced growers who are doing this, or has already educated themselves in the subject. nothing is for sale, there is no competitor, as I sell nothing to the public. buy up a kit , cross your fingers that carrots and orchids are the same mix ratios, post some pics, we can share notes of your experiments, supplier lists, links even. most components in a school kit are in your cupboard. for the cost of the kit, you can have a year of hormones, or better genetics to start with, just a thought.

piss on your pic demands, until others speak up. I'm not gonna get crucified here, just to pave the way for defendants to confess. this works, maybe its better that you dont accept that, chances are, you'd be the one to fuck up the genetics in a permanent way. it is an incredible responsibility to alter life in some of these manners, and most of you have been weighed, and found wanting.

good luck

 

[jcdws602]

I believe it works......I just want to see how it works....all I hear is all this talk but no proof and no directions on how to do this...if you are only sharing info........ well by all means share.......

 

[pharmacoping]

all the learning one needs to get started. after you've acquired the necessary equipment, and ready to mix, let me know, and I'll guide you through the mix process/ratios. I cannot cover years of research in a forum that attacks new technology like this, but these links should catch up the smart ones among us. as you see this information is very costly, and took me years of research to locate, before they were at your fingertips, and shared in forums. don't be scared, it is real, it is happening, even while you disbelieve, right here, and in home labs nationwide.

peace



http://www.sigmaaldrich.com/life-science/molecular-biology/plant-biotechnology/tissue-culture-protocols.html
http://gmocannabiswatch.blogspot.com/2010_12_01_archive.html
http://diybio.org/
http://www.springer.com/life+sciences/plant+sciences/journal/11240
http://books.google.com/books/about/Plant_tissue_culture_manual.html?id=Pw9FmOyNbbAC
http://www.pgec.usda.gov/mccormick/mccormick/publications/tomatotransformation.pdf
http://www.propagatingplants.org/TCHowWhy01.html

 

[pharmacoping]

http://www.biotech.iastate.edu/lab_protocols/AV_Micropropagation.html

 

[Guile]

Dude, I want to apologize.. I hate being harsh and reading back over my posts from yesterday it seems I had been, Sorry for that.
When you stated that you were producing/selling kits, it caused me to worry that you were selling something..... The kind of thing that might be expensive, complicated and not necessarily provide the sort of results that the end user might hope for biased on their investment.

I find plant tissue culture to be interesting stuff and one of these days I am probably going to make an honest effort to explore it first hand (I kinda like the idea of creating my own test-tube babies, and I think one day it might become practical).

I see the application, infact my first exposure to this sort of thing dealt with an enormous state of the art greenhouse where they were cloning a particularly unique award winning flower (honestly don't remember what it was, wasn't big into flowers at the time). I want to say that they carved off a small piece of the node material dipped it in a solution to clean (maybe an initial hormone treatment too) and placed into a test tube containing about 1/2" of gel in the bottom. They went into some kind of climate controlled cabinet and the next thing I saw was enormous tables of identical flowers that were to be auctioned off at a premium to florists and such. (obviously there is quite a bit that I didn't see)

The stuff I have been seeing lately while reading about hormones looks a little different like the plant matter is run through a blender or something (indistinguishable as to its origin though collected from arias that establish new growth). I have no idea what processes are used to get things to that point, I can only assume its a bit involved. The plants they establish appear to spring from a lump of plant tissue and gel with no discernible roots (atleast from the images I've seen) and I am under the impression that they need to undergo a complete change in hormone treatment and environment to establish roots (Ive seen mention if it, just never looked further into it). I can only speculate that the predominant hormone action needs to be focused either on plant or root growth at any given time (as though trying to force the cells to do both at the same time might lead to conflicts or other issues).

I would love to eliminate the need for mother plants in my garden, everyone in this country knows to keep total plant count under 100 so it would be nice to obtain all the genetic material needed from currently flowering or freshly harvested plants (especially if you are growing multiple strains).

I grow sea of green with multiple strains per table so I tend to top/trim side branches to get everything behaving as well as possible under their shared light source (and minimize popcorn buds). I have been successful rooting many of these cuttings and believe that if I could get down to 8 strains or less I could probably maintain my perpetual harvest utilizing no mother plants at all (it would suck if anything went wrong though).

Keep experimenting and exploring... I would love to see you start a guide thread here listing everything required to successfully repeat your results in my home (not an overpriced fancy kit, but what is actually necessary) might be just the thing to get more of us working with this sort of thing..

By the way, thanks for the links.. Some of them look like they will make for good reading

 

[pharmacoping]

jcdws602 - I will help you culture, if thats your intention. I cannot convince you of successes, or even teach you how to do this. I probably will not start a journal or a how to list thread..reason. I am a really positive dude, and the negativity is way too much for me to handle. I understand curiosity, and wonder, but the attitude of most of the responses(many whom have not even considered culture before this, is beyond my comprehension. Somebody suggested I chat with some pros in this forum, and I ended up with argumentative thumb sucking brats, who would argue the earths shape, as long as someone would take a stance. I have nothing to prove, or gain by sharing my experiences. I dont want a prize, or a whoa dude!, or really anything else but to share experiences with likeminded cultivars. If awareness of this technology is missing from the cultivars mind, then we are not like minded. I provided many links, enough to teach you every single thing needed to perform this, with nothing but ridicule, until the most recent post, and the "zoo of bacteria guy" who knows the possibilities. He asked for pics from one who says they have friends who do this, and fail, and I see none, nor is he suspect, possibly because of plausibility orsecond hand failures being disproved. Read the books, spend a couple hundies on literature, then come back here, and lets culture. If I laid out a how to I can only imagine that every step alo. ng the way will need to be broken down,proven, photographed, into hundreds of more threads. whos got the fuggin time, except you all, evidently. Please, after finishing your education, write us up a how to guide, with this extra time, but dont waste mine.

" An error does not become truth by reason of multiplied propagation, nor does truth become error because nobody sees it. Truth stands, even if there be no public support. It is self sustained."
“First they ignore you, then they laugh at you, then they fight you, then you win.” ....I'm almost there, but it is not worth the fight afterall, sorry.

 

[pharmacoping]

Guile,

thank you, and respectfully accepted. There are 3(I think) such lists in the links I've provided. Your Laminar Flow Hood can be mocked up by you.

The two main factors here are sterilizing the explant you've chosen, and settling on the proper mix ratios of agar,hormones, acids,foods. My (10%, obviously a rough estimate that could be as high as 40%, or as low as 1% even) contamination issues happen because the explant(node,leaf,flower,seed,etc) has either been A(sterilized so much that it retains useless dna, does not flourish, and/or B) has not been sterilized enough to remove unwanted materials. Both are visible within a day or two, and neither are a bummer when hundreds are at play. A liter or two will fill dozens of containers properly, and standby sterlized incubators are standing by, previously mixed for-rooting/dividing/shooting. Once my initial transfer is complete, I have had no issues with subsequent transfers, including mold. My sterile techniques are acceptable, to the level of propagation necessary for me to continue, and failure costs are negligible. For someone with intimate knowledge to say that "my buddies all went to mold, and the process does not work", is very very foolish, and couterproductive, however very interesting that I would have many friends here if I was attacking an emerging technology that I know I could never fathom myself, and challenging the reporter every step along the way, rather than encouraging a brother------to these men, with child like minds, move aside please, and let intelligence guide your way, until your blindness subsides.
ground up roots will perform the same, with some luck, but I do not do this. I have used mash years and years ago, because that was the only damn info available. It is very hard to sterilize a spoon full of mashed potato like plant material, and also difficult to keep it sterile while "mashing" it up. although for other species maybe, but for mj it is not necessary.
If inspired to try, dont do it on my behalf, or because of all the misinformation I may be spreading, but because you know you can do it, and even learn something along the way. If you think you can, or can't, you're right, either way.
Had I located a forum with a person claiming they found a way to beat the plant count,at a time when I was trying to learn new things in an emerging industry, I surely would not spew ridicule and spread doubts amongst the forum readership, and I say shame on all of you, for following your egotistical, misaligned instincts. I do thank you for identifying yourselves quickly though, and hope anybody else that figures out how to sex a seed before planting, move out of their moms basement, pay their rent, or even cure a disease, does not have to be exposed to the same.

 

[pharmacoping]

For those too lazy to read the whole thread, I DO NOT HAVE ANYTHING FOR SALE. I am not selling anything to you. i WILL NOT SELL TO YOU, OR YOU EITHER. i HAVE NEVER MADE ROSES WITH THC, OR TENDRILS. I HAVE NEVER INSERTED A BIOLUMINESCENCE GENE INTO MARIJUANA. i HAVE SEEN IT DONE THOUGH. i WAS NOT ALLOWED TO PHOTOGRAPH THAT. I AM NOT EXPERIMENTING WITH TISSUE CULTURE ANYMORE. I AM NOT TRYING NEW THINGS WITH TISSUE CULTURE. I HAVE BEEN PERFORMING THE SAME PROCEDURE, FOR THE SAME PURPOSES, FOR MANY YEARS. i AM SUCCESSFUL, AT TISSUE CULTURE,MARIJUANA CULTIVATION,MARRIAGE, FATHERHOOD, AND HAVE A REALLY BIG DICK TOO, IN CASE YOU WONDERED....i MIGHT EVEN HAVE A PICTURE OF IT SOMEWHERE...YOU KNOW, FOR PROOF AND ALL.... LETS SEE.... OH YEAH, MY AVATAR IS THE FLOWERED STRAIN OF PLUSHBERRY, AND DID SPEND HER EARLY LIFE IN A JAR, SHE ACTUALLY SHARED A JAR WITH 14 OTHER LOVELY LADIES OF THE SAME. THEY ALL GREW NEARLY IDENTICAL, AND THE HIGH IS EXQUISITE. i DID NOT CREATE HER, ONLY NOURISHED AND HOUSED HER. i DO THANK NATURE DAILY, AND SO SHOULD YOU

 

[pharmacoping]

MY level of experimentation has ended a couple years ago, when I was consistantly successful at my goal. I have no reason to manipulate genes in an already perfect plant. Every plushberry I ever grew, and everyone I will ever grow, will be near identical because of my efforts. Imagine if we were respectful from the beginning how far along we would be by now, as a forum, and in humanity.

 

[pharmacoping]

really?

Spam is the use of electronic messaging systems (including most broadcast media, digital delivery systems) to send unsolicited bulk messages indiscriminately.

thats not at all what I've done here. go get a belt, pull your pants up, tuck in your undies, maybe find work too.