So i find genetics very interesting. I'm more interested taking genes from one plant and inserting them into another one (not via breeding). Not sure how to word this correctly. Anyone out there doing this? Would love to hear and just know a little more. I plan on starting to take some classes at my local college, but other than that don't know much. Any help would be appreciated.
i want to learn genetics
- Thread starter patient76
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SCARHOLE
Well-Known Member
I assume you read "the biotechnology of cannabis sativa" primer I posted under "how to make your own genetically modified cannabis (in the advanced cultivation section)
I'm dreaming of school again to learn this also.
Check out the "Kahn academies " biology classes
http://www.khanacademy.org/video/dna?playlist=Biology
The process isn't to hard, is mainly about sterile environment an tissue cultures.
I'd like to streamline the process of genetic modification.
There has to be a way to make it easier.
Shrooms required lab equipment tell the Pf tech figured out how to make em easier.
I'm dreaming of school again to learn this also.
Check out the "Kahn academies " biology classes
http://www.khanacademy.org/video/dna?playlist=Biology
The process isn't to hard, is mainly about sterile environment an tissue cultures.
I'd like to streamline the process of genetic modification.
There has to be a way to make it easier.
Shrooms required lab equipment tell the Pf tech figured out how to make em easier.
SCARHOLE
Well-Known Member
The "kitchen culture kit"
Seems like my starting Point.
http://www.kitchenculturekit.com/index.htm
Now to find sources of Agrobacterium an the genetic material to introduce to the plant an the auxiums for callus an root growth...
Seems like my starting Point.
http://www.kitchenculturekit.com/index.htm
Now to find sources of Agrobacterium an the genetic material to introduce to the plant an the auxiums for callus an root growth...
SCARHOLE
Well-Known Member
In the middle of tissue callus formation Agrobacterium with added genes is mixed in the tissue culture.
The bacteria mixes it's self an the additional genes into the plants DNA.
Then the bacterium is sterilized out.
An root formation is triggered with auxioms.
Continue with normal tissue culture.
The bacteria mixes it's self an the additional genes into the plants DNA.
Then the bacterium is sterilized out.
An root formation is triggered with auxioms.
Continue with normal tissue culture.
The Chemist Brothers
Active Member
We need more scientific research and openness to this kind of critical experimentation with cannabis, otherwise i think things will stagnate themselves and people will just be renaming strains. i wanna see thc producing watermellons, fuck i want SOME GMO's!!!!!!!!!!! give me chronic grapes or give me some chronic... im looking to work in biochemical engineering, and it would be incredible to find a way to give plants a few genes to produce cannabanoids effectively and without the need for difficult extraction. some thc orange juice would be an incredibly effective way to move past the stigmatic bs Marijuana has to deal with.
hazey grapes
Well-Known Member
you're talking about
gene splicing & genetic engineering
that's some pretty freakin' AMBITIOUS stuff! if you DO get into that, fuckin' A, decode the marijuana genome and figure out how to get rid of all of the indica CBD, CBN and other stoney cannabinoids without having to grow huge IBL sativas! a no odor trippy 15 inch autoflowering trippy cambodian would be AWESOME. to this day, no one's been able to retain a true sativa buzz in a compact plant
i'm kinda inclinded to think it's just god's way of saying "good things come to those that wait". LOL
gene splicing & genetic engineering
that's some pretty freakin' AMBITIOUS stuff! if you DO get into that, fuckin' A, decode the marijuana genome and figure out how to get rid of all of the indica CBD, CBN and other stoney cannabinoids without having to grow huge IBL sativas! a no odor trippy 15 inch autoflowering trippy cambodian would be AWESOME. to this day, no one's been able to retain a true sativa buzz in a compact plant
i'm kinda inclinded to think it's just god's way of saying "good things come to those that wait". LOL
fucking HYSTERICAL! but watermellons? really? yeah man... i'd like to score some watermellon sinsemilla man! i can think of better fruit. i'd go for trippy naval oranges myself. really, it wouldn't work though. you still need heat to activate THC. i learned that the hard way when i ate an entire gram of bud once.that's why people make hash brownies. i really want to try that some time when i have a head stash.
jewgrow
Well-Known Member
As much as I enjoy the idea of cannabinoids in fruits via genetically modification, I don't think it would be all that feasible with edible crops. Even if you successfully got the cannabinoid producing gene(s) in the plant, the cannabinoids are in the trichomes on the outside of the plant tissue. Wouldn't it be great to bake a nice apple pie and get the whole family high though? I suppose the only way to do it is figure out how to get the cannabinoids to grow in specialized structures IN the fruit of a plant (or maybe tuber).
ismokealotofpot
New Member
God is perfect man is not. Man made beer God made pot. Why dont you just find two plants you like and crossbreed.
Help me out with this..... so i did read the info you put up about manipulating the genes and i get the part of how agrobacterium transfers the dna to the callus... but tell me this...how does one figure out how to pull the gene from one plant? Or is it a matter of turning ON a gene within the cannabis plant? It's like why does the white widow have so many more trichs then say one that doesn't?
This shit can't be that complicated. Sure it sounds like it right now because we just don't understand it. I truely believe anyone can do anything they want if you really want it. So my fat ass is heading back to school to make the most amazing cash crop us stoners could ever want!! Trichs up the ass, huge buds, short, and the damn thing smells like a lemon tree!!! Anything is possible just gotta put in the time.
Thanks for the videos as well i'll check em out later.
SCARHOLE
Well-Known Member
The genes are simply purchased .
Already mixed with the Agrobacterium when purchased from Roche / genteck ect.
Like Buying spore syringes
But I'd like make my own , Wich is where college comes in.
All genes have controllers, if you could turn the THC genes all the way up ,imagine how white it could get.
Already mixed with the Agrobacterium when purchased from Roche / genteck ect.
Like Buying spore syringes
But I'd like make my own , Wich is where college comes in.
All genes have controllers, if you could turn the THC genes all the way up ,imagine how white it could get.
The Chemist Brothers
Active Member
god is imaginary, like the idea of perfection, and yea.... thats all i gotta say about that.
SCARHOLE
Well-Known Member
An improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation.
http://www.plantmethods.com/content/2/1/16
To quote Ian Malcolm from Jurassic Park "You create new life forms about which you know nothing at all... You create many of them in a very short time; you never learn anything about them. Yet you expect them to do your bidding, because you made them and you therefore think you own them; you forget that they are alive..."
http://www.plantmethods.com/content/2/1/16
To quote Ian Malcolm from Jurassic Park "You create new life forms about which you know nothing at all... You create many of them in a very short time; you never learn anything about them. Yet you expect them to do your bidding, because you made them and you therefore think you own them; you forget that they are alive..."
do you happen to know if the full genome of the cannabis plant has been published? I know parts of it was but i don't think the whole thing was.
i'm gonna try to contact that company and see if they could help me get started. I'm down to start with something small and get things right before i go full bore.
Was thinking of inserting the GFP gene into the plant so that when the metal halide lights are on (blue) the leaves will put off a green light
i'm gonna try to contact that company and see if they could help me get started. I'm down to start with something small and get things right before i go full bore.
Was thinking of inserting the GFP gene into the plant so that when the metal halide lights are on (blue) the leaves will put off a green light
SCARHOLE
Well-Known Member
I believe it requires black light to make em glow.
That would me my first cross also.
Just a way to visually see if it had transfered genes.
This site has alof of required growth hormones, antimicrobials, an more in small quantities.
http://www.phytotechlab.com/
I'm currently having trouble getting a grant from my wife to begin my studies.
Maybe after x mas.
I believe the full genome of cannabis is on the net.
I'll have to look.
Got a friend whose brother has a biotech masters, an may be around for thanksgiving.
Love to get is input on home gmo feasibility.
That would me my first cross also.
Just a way to visually see if it had transfered genes.
This site has alof of required growth hormones, antimicrobials, an more in small quantities.
http://www.phytotechlab.com/
I'm currently having trouble getting a grant from my wife to begin my studies.
Maybe after x mas.
I believe the full genome of cannabis is on the net.
I'll have to look.
Got a friend whose brother has a biotech masters, an may be around for thanksgiving.
Love to get is input on home gmo feasibility.
SCARHOLE
Well-Known Member
rollinronan
Well-Known Member
biotech pays well and is a very new field so that industry will be hiring for the forseeable future
as for the GMO plants, hmm well the question i have as a qualified person is; how much THC production can a plant (any plant) handle without wrecking the it's metabolisim? producing too much of anything can lead to death. the process of gene targeting has been around afew years and the genes relevent for THC production (i say production and not expression because THC is not a protien and only protiens are expressed) have no doubt been sequenced, targeted, isolated and stored in a vector somewhere, im no expert on plant biotechnology,in all i have learned, i have used either microbial or mammalian cell lines where plasmids, cosmids and YACs ect. are used not agrobacterium, however im sure the principle of transformation/transfection in plants is quite similar. ill do my best to answer some questions if ye have any?