So no pictures?
I purchase lab cultured hemianthus callitichoides for my aquarium but lab cultured pot plants just sounds like silliness. Perhaps a good way to illicitly transport clone only strains but a bit of a joke when it comes to everything else. I know several people experimenting with cannabis cultures and although it is possible it just isn't practical. If you do manage to have a culture both root and create leaves without molding the likelihood of successfully transferring the plant from a sterile growth medium to a regular pot is damn near zero. When successful the plants are extremely weird looking and take months and months to develop to the point that you can take a successful clone. Yes it is possible but seeds > tissue cultures on way too many levels to list.
And yes, proof matters when making crazy claims. No pictures = no proof makes a ton of sense to me.
Especially with shit like this:
Picture of glow in the dark MJ please.Originally Posted by pharmacoping
Last edited by Gastanker; 01-17-2012 at 06:00 PM.
I'll second. You don't need macro for glow in the dark marijuana.
Optimistically, you have it nailed. Honestly, you come across as a scam artist. Definately do some work with a PR group if your wild claims are factually based.
Nothing to see here...Move along
tissue culture
Once again for the illiterate among us..I DO NOT MANIPULATE THIS WAY. I HAVE NEVER MADE A GLOW IN THE DARK marijuana PLANT. I have seen this though, done with using the dna of a jelly fish that illuminates.You can buy these genes for manipulation. They are available, as well as hundreds of amino acids to change/manage/cause genetic changes in your mix, they are available,and many are already patented, for marijuana. I didnt invent this. I do not have photos of it. you do not have to believe it either. I am here to talk to professional cultivators about cutting edge growing science, not to convince you that genetic manipulation is real, and real old news. I do this, have done this, will continue to do this in my garden. I have also learned hundreds of different mixes to not use, hundreds of ratios that dont work, vegging techniques that dont work, and hardening off techniques that dont work. I use four hormones, and a preservative. If sterile technique is used, there is no reason to have "mold" in or on your plant. I lose 10% of my explants within the first five days,as bleaching/or otherwise sterilizing the pieces is a critical time for the sample. after that I have never seen mold in a dish, or a vegging plant from a dish. My cuts are bleached, for 15-20 minutes, stirred with a machine, then sterile rinsed,agitated, then rinsed in 70%alcohol for a few minutes,sterile water then transferred to a sterile home,under sterile positive hepa air conditions, that stays sterile. whenever I've failed, I keep trying until I've reached my goal, to bad for your buddies, they were so close..
i use 30-50 jars/tubes. one makes 4 more jars worth in 3 weeks, then they do the same, and so on, adding to as many as desired. We have no idea what hormone/nutrient mixes/sterile techniques etc your culturing friends used, but we do know they are not sterile, each and every time they transfered, I wonder why? because these molds and bacteria will affect your plant in adverse ways, and most likely kill the weak ones. If they make it that far, hell, they'll even look funny and take forever to establish, making one think again, about your friends' techniques. But at least they admit, and embrace the new technology, and commit to trying it out for themselves. A+ to them warriors. my plants do not look much different that yours my friend.
for you serious ones though, maybe keep using private messages. it's great to hear from you and hope I can help.
I know it's risky here, but if anyone else has done this, you dont have to show your face, but please pm me?
Yeah... the guys I knew were using a sterile university lab... But you are saying you have 90% success in a home made lab? And you are suggesting others can have the same 90% success using a home made lab? Or do you suggest most growers invest big bucks on a professional grade lab? I see pictures of cloned/seed grown plants and bud, where are the pictures of the developing tissue cultures? If you have personally seen glow in the dark MJ why can't you get pictures?
Mind posting a whole grow?
Last edited by Gastanker; 01-17-2012 at 08:11 PM.
imagining my pictures will be stolen or something, right? not sure how things can be proved. I'm not a filmographer, I do not have thousands of shots to show off at a internet forum, I dont have film, elapsed or otherwise, I'm not on ebay. no wild claims here, except this beats the plant count, thats all. successfully . and that I am not the only one doing this, even amongst your readership. backoff with the proof thing, I cant win. no scam artist here, not wanting money, or a prize, only to meet others, that make these miracles happen within their home. I am immediately made aware of the reason they overlook this forum when considering sharing with others, or learning more themselves. I sure hope my photo skills suffice, albeit not proof of anything really. all pics you see are simple tissue culture, in a few different stages, even the end results, which are not much different the the great weed you all grow, but I got my max 72 in flower and veg, and none in clone, except monthly, for a couple days. it works well for me, and a few fortunate persistant people, it does not have to work for you, nor do you have to give it a second thought, but when you do hear about it, send me a posi vibe please. I do have garden pics too, and even some stellar photos of Riot seeds in flower, as promised some time ago. if anyone cares, pm me. all pics at this thread, thusfar, were taken today or recently, and are all from a jar or test tube, originally grown from callus. whats it gonna take?
Im with you ^^^ herd about this years ago anyone with netflixs should know about this big breeders are already on it!
800 watt perpetual grow here!
By all means tissue cultures are possible but very very few people have success with cannabis.
You are making wild claims and you are trying to sell things - reread your first post; you both state you make kits for sale and go on to say you have seen glow in the dark cannabis as well as THC laden roses. Those are WILD claims. To say that you can go from a tissue culture to a rooted regular plant in days is also a WILD claim.
I would love to hear more about your method but please post about your method with substantial photo documentation to back it up - not wild claims and begging for pms (this sounds like you are trying to sell something). I would love to see a day by day progression from tissue culture to plant. You have 70 on their way? Please show us these photos! But please don't just post 70 plants as those of us who understand the process of tissue culture know that they look drastically different than a regular plant. Absolutely no need to show bud shots as they are really inconsequential.
Last edited by Gastanker; 01-17-2012 at 08:40 PM.
let me rephrase, I have seen hundreds of clones/plants come from a dish, but never seen mold on or around them. by the time they are this mature, they seem much more resistant, even able to harden off with the lid off.
gastanker,
I do often get 90% success, during the first transfer of material, but typically it is 100% thereafter. I am not suggesting that anybody can do this, as we know by visiting the newbie forums, some cannot even cultivate, let alone market/plan a grow. this is what i do. i came to meet others that do it. I have made starter kits, with instructions for people, for money, thought I'd cover that . I recalled that offer in a previous post, after some pm's with requests, for my own reasons. I will share links that I come across though. I'm non combative, and really not into the whole "defend every post" game, as evidenced in many many areas in this forum. I really want to meet some class act cultivars, email chat only. but this might do it eventually.
also, again, each pic was started in a dish, regardless of your fears, or misinformation, or even experiments you may be privy to. growers, most wold not invest in an expensive lab, thats silly to suggest, but on par with the peer majority. I've explained how, and where I do this. it is not expensive. the tent was less than 500$pressure cooker80. no secrets here, i saw the same results you did, alot, but not now, thats all, for years. most commercial foods produced by monsanto seed corp are done this way, its not voodoo, dont be scared. these same people are the ones that are responsible for demonizing this plant in the first place. it worked too. "they're not so big you know, you're just on your knees." dont spend your money unless you are sure you can do it.........if you think you can do it, or not...either way, you are right !
#1 is callus, dozens of callus, made to root immediatly are cut from this every few days, or as needed to refill the rooting jars, which are refilling the sprouting jars. which are refilling the veg trays, which refill the flowering trays. these stages are shown in the same pic, old cam, but visible. rooting area is light sensitive, so pics will be sporatic. veg pics comin soon
I sure wish I had the skills to recognize a plants origins by its stature, whether cloned or manufactured, or??? I try, but all the banana tree look the same to me, grown in a test tube, or the dirt. in FL, or online is proof.you can buy tissue culture bana trees, orchids, venus fly traps, some are ony sold this way. some cannot be shiipped unless in a sterile medium sealed vessel.
nitenite, some of us work all day, not sit in class. but i ove ya man, you'll see soon enough whats going on
http://buymarijuanaseeds.com/communi...rijuana.16304/
http://www.washingtonpost.com/blogs/...89MK_blog.html
http://blogs.scientificamerican.com/...the-dark-cats/
http://www.time.com/time/magazine/ar...962873,00.html
now, can we move forward. of course the reporters did not talk with anyone but the large labs. but this should put most at ease. we can only hope
You've convinced me! Major labs made glow in the dark cats so they much be working on marijuana and I'm sure you were there to witness it! I want a kit! How much! How much!
Seriously, how much are you charging for your tissue culture kits? I know of several competitors - can you beat them?
I grew rootless tobacco plants from such tissue cultures in Erlenmeyer flasks in the late 70s when I was in college. Least differentiated form looked like apple sauce, the next form looked like corn pops and finally the actual plant. All were grown in different agar solutions based on the type needed. I grew thousands for radioactive electrophoresis experiments for biochemist Professor Chong Maw Chen at the University of Wisconsin-Parkside. It would be easy to have such lab at home. Just need an autoclave and UV-lighting booth for transfers...and there was ALWAYS some contamination. I used to keep a zoo of different infections that occurred. The coolest looked like a putting green with little stalks that had red balls on top. There is no reason the same couldn't be done with marijuana.
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