Effect of vitamin C and folic acid on seed vigour response and phenolic-linked antioxidant activity.

Burguieres E, McCue P, Kwon YI, Shetty K
Program in Plant Biology, University of Massachusetts, Amherst, MA 01003, USA.

Folic acid and vitamin C were used in the concentration range of 0-500muM as exogenous growth enhancers to stimulate pea (Pisum sativum) seedling vigour. The results suggest that a concentration of 50muM folic acid and 500muM vitamin C were optimum in maximally enhancing seed vigour and potentially seedling performance according to both agronomic and biochemical seed vigour parameters. Results indicated that germination percentage, shoot weight, shoot height, and root length were enhanced in folic acid and vitamin C treated plants compared to control plants. The levels of enhanced phenolic content in response to folic acid and vitamin C treatments were highest on days 8 and 10. Evaluation of critical biochemical parameters indicated that the average glucose-6-phosphate dehydrogenase (G6PDH [?]) activity and proline content in response to treatments were higher than control and correlated to enhanced phenolic content and DPPH-based antioxidant activity. Key enzymes, guaiacol peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) were also higher in response to treatments and correlated to enhanced phenolic content and DPPH-based antioxidant activity. Taken together, these studies support the hypothesis that the proline-linked pentose phosphate pathway stimulates phenolic synthesis and related free-radical scavenging antioxidant activity. Further, this proline-linked pentose phosphate pathway stimulation in response to folic acid and vitamin C was also correlated to antioxidant enzyme response indicated by the stimulation of GPX, SOD, and CAT activities. Therefore, this study indicates the enhancement of seed vigour response by folic acid and vitamin C as reflected in both agronomic and biochemical responses, and this occurred through the stimulation of phenolic-linked antioxidant response that is likely positively modulated through the proline-linked pentose phosphate pathway.

Bioresour. Technol. (2007)

I am a user of Folic Acid. I have had 100 percent success with 20 seeds I received. The plants love.......wether it be inside or out. I buy the 1 mg's and I give one pill disolved in it's ferts and I give it to 'em.

Go to your local Pharmacy, it's on the vitamin shelf. Known also as B9.
Pregnant women take that supplement.................check the label and get the ! mg's. Costs about 3 bucks for 90 pills............dissolve in warm water, then add your regular ferts.
If you don't see the Folic Acid on the shelf ask the Pharmacist.....maybe he has it behind the counter to control how much us growers use don't confuse it with fulvic acid.

Dissolve 1 pill per litre of water, that's what I'm doing.

To dissolve the pills, first take the hammer to em, then put in very warm water. They dissolve quite quickly, once dissolved add to your water that has been sitting for 24 hours+

I'll need to read through the technical data at a later date. I hit the charlie and viagra last night and I haven't had much sleep.

It sounds very interesting, although all my addled mind has been able to gleen so far is that it promtes more rigorous growth. My gf should have some of these lying around.

I'm really interested in the antioxidant scavenging part. Antioxidants are known to promote longer cell-life, even in humans. Tea is full of them by the way. Hmmm, I could have something there.

what does this do?

Further, this proline-linked pentose phosphate pathway stimulation in response to folic acid and vitamin C was also correlated to antioxidant enzyme response indicated by the stimulation of GPX, SOD, and CAT activities. Therefore, this study indicates the enhancement of seed vigour response by folic acid and vitamin C as reflected in both agronomic and biochemical responses, and this occurred through the stimulation of phenolic-linked antioxidant response that is likely positively modulated through the proline-linked pentose phosphate pathway.

ummm... enzymes good. <----- is that simple enough for you

mogie, i tried similar approach a few month back and it failed for me, so i'm not so sure on this anymore. also ASA was suspected to increase the sprucing rate, that failed too for me.

so far the only successful method was the use of cloning hormone, that gave me a 100% rate with all i started till now. but i guess i gone give folic acid another try if it worked that well in your grow. maybe my dose was way to high...

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keep the thumb green and the gun oiled, !

Tell me what you think. Maybe I am thinking I see too many results here.

wop, gone head later to the store, got only mixed pills left from workin out, hopefully they got pure B9 besides the fillers

really, if it been workin that great in your grow it must been me using a to strong dose of folic acid.... we get all smarter one bright day i guess hopefully mine is coming soon

__________________
keep the thumb green and the gun oiled, !