Originally Posted by Cann Don't know how I forgot to mention Mycelium Running - Paul Stamets' book which IMO is a must read for anyone on this planet, especially those concerned with the earth and organics. If you don't own a copy, pick one up right now. Also pick up a copy of Teaming with Microbes by Jeff Lowenfels and Wayne Lewis, and if you're really keen grab a copy of Permaculture: A Designers Manual by Bill Mollison - which is literally my bible. Hope this helps someone out there...
Originally Posted by nugbuckets From there the 4 total axial leaders are left to grow for a spell (all strains will vary in length of time).......
Originally Posted by nugbuckets Main-Lining Process...Up Close...(because you asked)........now pay attention.... ......the young plant is 7 inches tall working on it's 7th node....it has spent the last 5 days in a 12/12 cycle until she (in this case) threw a tiny female pre-calyx....two nodes are chosen, one (upper) for the "hub", and a second (lower) for clones.....everything else above and below the two chosen nodes is removed leaving this, it is then transplanted into a 2 gal pot to veg. ...
Originally Posted by TMB77 Hey all well, didnt have time to read the ten middle pages of this thread, but...I would like to add my two cents. So, i've been working in tissue culture for the past two years, and...this is very possible. the things is, fan leaves are (probably) too old to display the type of totipotency (the ability for a single cell to regenerate into a whole new plant) that is needed. Here is how you would do it: Grow your seed until it is the ...