THC producing plant that doesnt look like marijuana

bts420 · #1 04-25-2008, 10:40 PM

Ok. Heres the idea. We would like to create a plant that produces large amounts of THC, but does not look, smell, or have physical properties of the cannabis plant. My thoughts on this: We need to start from a marijuana plant, because thats the only plant we know that produces THC in the amounts we want. This is just something we were wondering about and were not able to find info. on. Maybe start a sub forum or something that goes along with this kind of idea. thx for any info =)

AlphaNoN · #2 04-25-2008, 11:04 PM

Biochem 101: How to design a Cannabis-equivalent citrus plant Step One:
Biochemically isolate all the required enzymes for the production of THC.
Step Two:
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
Step Three:
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
Step Four:
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.

iblazethatkush · #3 04-25-2008, 11:43 PM

Dude, this would be so awesome. You don't know how much money I would pay for one seed of plant like this

. This shit is way over my head, tho. I'm interested in seeing what some smarter ppl on here think.

ganjagoddess · #4 04-25-2008, 11:45 PM

hahaha Like any Stoner is gonna Try to do that too a bunch of plants.

Theoretically it wouldnt be passable down through the seeds would it, you would have to do that to each and every plant you want to make THC.

iblazethatkush · #5 04-25-2008, 11:47 PM

Quote:

Originally Posted by ganjagoddess

hahaha Like any Stoner is gonna Try to do that too a bunch of plants.

Theoretically it wouldnt be passable down through the seeds would it, you would have to do that to each and every plant you want to make THC.

Good point. It has to be possible somehow. I would love to talk to a plant scientist (forgot the name lol) and see if it's theoretically possible. If so, I think I found my life's calling

Thanks for the thread + rep.

AlphaNoN · #6 04-26-2008, 01:49 AM

Quote:

Originally Posted by iblazethatkush

Good point. It has to be possible somehow. I would love to talk to a plant scientist (forgot the name lol) and see if it's theoretically possible. If so, I think I found my life's calling

Thanks for the thread + rep.

LoL "plant scientist" (botanist). But if you did indeed insert the necessary gene to produce THC into another plant via bacterial vector it would pass it on to it's offspring, that's how Monsanto created it's RoundUp Ready crops. An interesting side effect would be that once released into the wild your hybrid would "infect" other plants of the same species via cross pollination causing a percentage of their offspring to produce THC as well.

ganjagoddess · #7 04-26-2008, 02:02 AM

Alpha you seem well versed in Bio Chemistry.

But Why has this not happened yet...

I would think someone somewhere would have done it...

Think of it every fruit you eat from now on gets you stoned...

The medical Patients would be all over it...

I do not see how you could affect its reproductive traits, since they were set at the "birth" of the plant. You are only imposing a gentic mutation essentialy right?

The only way I thought it would be possible to actually procreate a "Marijuana 2" was to map its entire Gene Structure and let Genetisists go to town.

Much like they did the strawberry/Fish Blend (Cold weather Fruit)...ect..

You would have to buy the genetic blueprint before even thinking about altering it.

I dont know spell it out for me, I am rather slow, but very interested.

Thanks and cheers...

AlphaNoN · #8 04-26-2008, 02:20 AM

It's very doable, but the investment needed to undertake such an operation is mind boggling. Finding, isolating, and extracting the genes that control THC production, then finding a suitable agro-bacteria to carry it into an equally suitable host plant would cost into the hundreds of millions. Only companies (such as monsanto) would be in a position to do such a thing, and they, of course, have no interest in a plant that would screw up the profit margins of their other products, or possibly infect a staple food product with an intoxicating substance.

If you need proof of it's reproductive capabilities just google "RoundUp Ready cross pollination" or a variation of those terms. People are afraid of it because Monsanto owns the patent on the very genetic makeup of their seeds, meaning that crops that have been cross pollinated and grown with "their genes" belong to the monsanto corporation.

PolyploidyPrince · #9 04-26-2008, 03:57 AM

"crops that have been cross pollinated and grown with "their genes" belong to the monsanto corporation."

Wow, thats pretty crazy.
And yeah, most people that have the resources for something like that aren't the least bit interested in Cannabis. I think the real the innovation in pot is going to be when someone stabilizes a polyploidy strain. I've been messing around with this idea for awhile now and it shouldn't be too long before you all start seeing polyploidy strains on the market.

storm22 · #10 04-26-2008, 12:04 PM

ive eaten a graple a apple that tastes like grapes