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  #231  
Old 07-06-2008, 08:50 AM
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Another source of cytokinins is coconut milk. You can find some recipes for tissue culture on google.

coconut milk tissue culture - Google Search
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  #232  
Old 07-06-2008, 09:51 AM
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these are good links you're supplying

my one concern with methods such as this is the unknown amount of the hormones in them. I cant seem to find any amounts (as in mg/l) or any molar concentrations. when i've done this before it was with known concentrations, so I was able to supply exactly 2mg/l cytokinins to 0.1mg/l auxin, the magical shoot regeneration formula. I suppose at worst it would take some experimenting with amounts to figure out what is needed.

anyways, good research tech, you planning on trying this yourself?
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  #233  
Old 07-06-2008, 10:39 AM
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good work impressive
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  #234  
Old 07-06-2008, 12:45 PM
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"anyways, good research tech, you planning on trying this yourself?"

Nah, I was going to get into years back and I went out and bought a bunch of books on the subject but the more I understood the more I realized that, for pot at least, it just wasn't worth the hassle considering how easy cloning is. Experimenting with cytokinins, auxins and gibberellins is interesting though.

I do, however, encourage people to try tissue culture; it could be a fun way to expand a hobby.
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  #235  
Old 07-07-2008, 12:12 AM
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Quote:
Originally Posted by MsMILFweed View Post
Is that you who has?? What happened after it rooted.. nothing. You just had a rooted leaf?


that is some Dank ass bud!
LOL
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  #236  
Old 07-07-2008, 01:29 AM
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TMB77:

What's your opinion on the methods from the original post? Do you think it's possible to propogate with the techniques that Tet and Milf tried? Or was Woo full of shit?


I just spent an hour reading this thread
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  #237  
Old 07-07-2008, 05:00 AM
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It was incomplete and overly optimistic



Fanleaf clone protocol: homegrown science
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  #238  
Old 07-07-2008, 10:06 AM
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ya know i have been watching this post and all i can say is in the time we have spend with it we could have harvested about a metric ton of sticky each from conventional cloning.

if it aint broke dont fix it.

by the way i am still waiting for the op to show us one example of this actually working as he claims he does constantly.

i suspect he is blowing smoke up our asses and finding all of this amusing.
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  #239  
Old 07-07-2008, 03:11 PM
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i just stumbled on something that may clear up some stuff

Super Starts Plant Tissue Culture - Home
it seems that a company makes a tissue culture kit. it aint cheap
the trick is to figure out what it is and reproduce it and bypass the 250 pricetag
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  #240  
Old 07-07-2008, 03:25 PM
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Quote:
Originally Posted by mrbuzzsaw View Post
i just stumbled on something that may clear up some stuff

Super Starts Plant Tissue Culture - Home
it seems that a company makes a tissue culture kit. it aint cheap
the trick is to figure out what it is and reproduce it and bypass the 250 pricetag
Includes:
30 Baby food Jars and PP tray
60 Tissue Culture plastic jars lids

4 Packs MS Media Mix - *soluble powdered nutrients*
4 Packs Gelling Agent - *agar, 'agar agar' can be found in many health stores.
4ml BA Shoot Multiplying Hormone - *cytokinins*
4 ml NAA Root Inducing Hormone - *Auxin*
4 ml PPM Preservative Mixture - *Probably just thiamine, but they might have stuck some other stuff in there*

Stainless Steel Forceps and Scalpel
Sealing Tape, Droppers and Measuring Spoons
pH Testing and Correction Kit
DVD or VHS video 50-page Illustrated Instructions with Recipes




hope any of that helped.
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