jriggs nice avatar "watershipdown" i havent seen that for 20 years brings back memories of childhood i use to watch that all the time. interesting work with those sprouts

How long does this process take?? I still don't think its any easier than pruning
a branch and tossing it in the cloner.

well, it took me a year to finally come up with this working protocol, now that i've figured all that stuff out it would take roughly 2 or 3 months to go from seed to rooted clone.

Still a lot easier to have a good mother plant and take clones from her.

I've never done a tissue culture (never saw the reason to) but this website has lots of info on the subject:

Kitchen Culture Kits - Intro Page

It seems like a pain in the butt compared to clones.

hey tmb77 thanks for the quick tutorial.I have had many leaves take root accidentally over the years.This prompted me to try quite a few different ways to clone them.I have to say in all the rooted leaves I've seen none have produced a plant. Previous to the culture post I was wondering if maybe the selected test leaves were already in the dieing phase of a leafs life.Your experience with the culture method makes me think this even more.Maybe the newest smallest leaflets should be used. Or perhaps forcing a flowering plant back into veg and using the first single blade leaves.Fun to think about these things , but overall I concur with most that unless you are going to tissue culture 1000's in the lab it's probably not worthwhile.

well ouizer, I really believe that people are not far off. if you can form roots you're halfway there...all you need to do after the roots have formed is supply a different hormone ratio to encourage fast shoot formation.

basically when the roots are forming you have a high Auxin:cytokinin ratio, if you can switch it to a high cytokinin:auxin ratio shoots should form. I'm currently trying to find cheap sources of cytokinins instead of buying the stuff my lab usually uses...cuz it's mad expensive.

I've not forgotten about all this...I'm hoping to start a new thread (including....PICTURES!) in the next few months where I actually make this happy.

Nitrozyme is a product that contains cytokinins.


Nitrozyme - Organic Fertilizer, Hydroponics, Diatomaceous Earth, Hydrogen Peroxide, Agricultural Fertilizers, Gardening Products

ah, nice find man...I might have to check that out.

although, it will have to wait till I feel like spending 30 bucks or so on an experiment for this crowd, I really dont have much use for the stuff myself. I was going to crack some old texts tonight to see if there is a local plant I can grab and process in some way to get the hormones I need, but it's nice to know I could just order some if i wanted. (kicking myself once again that I didnt grab some hormones from the lab before leaving for my new job)

tmb77 please help me you know your stuff so i figured id ask
ive been looking up micropropagation and every time i try to create sterile conditions the water ends up slightly yellow and the leaves die when i open up the test tubes they kind of smell bad
ive done dozens of cultures and kind of have limited materials(no access to rooting hormones or agar) but have done my best to provide nutrients for the cuttings

im open to anything please help

I need a lot more details than that, what exactly are you trying to do?

I gather you're using test tubes filled with water to try and propagate herb?

where are you getting the water from...the tap?

what nutrients?

what sterilization method?

what tissue from the plant, at what age of growth?