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Old 10-02-2009, 04:39 PM
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Default germination using colchicine solution
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well guys i usually refrain from posting in this section cause i dont want my noobish nature to be to obvious..
BUT ive been doing some reading recently about germination techniques using a very weak concentration (.04%) of colchicine in solution with distilled water.

has anybody tried this, and if so what type of results did you get...

im also curious where one can find this stuff for botanical use, and if you need a license to purchase it, as I understand that in high concentrations this stuff is extremely toxic...

see i'm the type of guy that doesnt have much space for things, and because of that i need something that has some very polyploidy traits if you know what i mean... and because im a) cheap, dont like spending 100's for seeds, and b) the type of person that likes to experiment and c) has lots of bagseed lying around. so i was wondering what i could do with bagseed and colchicine treatment.

any input is appreciated and thanks in advance!
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Old 10-02-2009, 06:45 PM
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Originally Posted by IAm5toned View Post
well guys i usually refrain from posting in this section cause i dont want my noobish nature to be to obvious..
BUT ive been doing some reading recently about germination techniques using a very weak concentration (.04%) of colchicine in solution with distilled water.

has anybody tried this, and if so what type of results did you get...

im also curious where one can find this stuff for botanical use, and if you need a license to purchase it, as I understand that in high concentrations this stuff is extremely toxic...

see i'm the type of guy that doesnt have much space for things, and because of that i need something that has some very polyploidy traits if you know what i mean... and because im a) cheap, dont like spending 100's for seeds, and b) the type of person that likes to experiment and c) has lots of bagseed lying around. so i was wondering what i could do with bagseed and colchicine treatment.

any input is appreciated and thanks in advance!
no need for it. i just germed over 30 seeds with the paper towel method. just the water. 100% success rate.
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Old 10-02-2009, 07:30 PM
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im not talking about using it too make the seeds germinate man... but thanks for the input.

this is something entirely different.
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Old 10-02-2009, 10:28 PM
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im not talking about using it too make the seeds germinate man... but thanks for the input.

this is something entirely different.
fill me in.
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Old 10-02-2009, 10:42 PM
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fill me in.
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Botanical use

Since chromosome segregation is driven by microtubules, colchicine is also used for inducing polyploidy in plant cells during cellular division by inhibiting chromosome segregation during meiosis; half the resulting gametes therefore contain no chromosomes, while the other half contain double the usual number of chromosomes (i.e., diploid instead of haploid as gametes usually are), and lead to embryos with double the usual number of chromosomes (i.e. tetraploid instead of diploid). While this would be fatal in animal cells, in plant cells it is not only usually well tolerated, but in fact frequently results in plants which are larger, hardier, faster growing, and in general more desirable than the normally diploid parents; for this reason, this type of genetic manipulation is frequently used in breeding plants commercially. In addition, when such a tetraploid plant is crossed with a diploid plant, the triploid offspring will be sterile, which may be commercially useful in itself by requiring growers to buy seed from the supplier, but also can often be induced to create a "seedless" fruit if pollinated (usually the triploid will also not produce pollen, therefore a diploid parent is needed to provide the pollen). This is the method used to create seedless watermelons, for instance. On the other hand, colchicine's ability to induce polyploidy can be exploited to render infertile hybrids fertile, as is done when breeding triticale from wheat and rye. Wheat is typically tetraploid and rye diploid, with the triploid hybrid infertile. Treatment with colchicine of triploid triticale gives fertile hexaploid triticale.
When used to induce polyploidy in plants, colchicine is usually applied to the plant as a cream. It has to be applied to a growth point of the plant, such as an apical tip, shoot or sucker. Seeds can be presoaked in a colchicine solution before planting. As colchicine is so dangerous, it is worth noting that doubling of chromosome numbers can occur spontaneously in nature, and not infrequently. The best place to look is in regenerating tissue. One way to induce it is to chop off the tops of plants and carefully examine the lateral shoots and suckers to see if any look different.[7] If there is no visual difference flow cytometry can be used for analysis.

in plain english it mutates the plant into producing more main branches (colas!) with bigger, more ptent buds... there was a guy back in the 40's that did a bunch of experiments trying to extract thc from plants but what he found out was that colchicine germinated plants were nearly double in potency.

i read another thread on here about someone using colchicine in concentrations of .1% however every serious scientific article ive found on it shows the best results at levels no higher than .04% concentration.
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Old 10-03-2009, 03:30 PM
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biggity bump, anyone?
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Old 10-04-2009, 01:54 PM
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wow i have never heard of this before but it sounds awesome. looking forward to seeing how it turns out.
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Old 10-28-2009, 09:19 PM
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I've done it and the plants do branch like crazy and the final product is potent.
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Old 10-28-2009, 09:23 PM
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where did you find your colchicine?

i cant seem to find a source for the stuff
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Old 10-29-2009, 09:06 AM
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where did you find your colchicine?

i cant seem to find a source for the stuff
A quick search turned up that in the US the stuff is most often perscribed in pill form fort gout and requires an RX, soooo go look up some symptoms and put on a show or buy some offline possibly? Seems like its gonna be pretty tough to get your hands on
 
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