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Old 09-14-2009, 10:44 PM
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Default once again...cloning from fan leaf
was thinking if u take a fan leaf ,slice it at the end of the petiole where u want shoots,put a mixture of cytokinin and auxins on the slice for shoot formation(2mg BAP,Kinetin,any cytokinin and.01mg IAA,NAA,any auxin).....then like usual put ur root gel on the other end and stick it in a dome.......
i dont see y it woulnt work....its more or less the same thing as tissue culture just minus all the bs.....
i already found where to get the hormones....i think im gonna try it
some chinese (kelong)guy is selling this method....called ternpc....google it
he doesnt say how he does it this is all i could think of

opinions???????
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Old 09-14-2009, 11:09 PM
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Is the cytokinin and auxins in the compound this guy has itself or do you get it elsewhere?

It sounds to me like it basically turns a small piece of 0.3-1cm length plant material into a seed. You will be able to get all your clones from a single mother, but instead of having a rooted 4 inch plant in a week, you have a rooted "seed".

As for most plants, an entire regenerated plant with developed roots will be got within 4-11 days. After 15-50 days training, it can be moved out of the garden for transplanting. Rapid propagation is factually realized.

Not sure what that '15-50 days training' is supposed to mean either.

Can't imagine the number vs time spent is efficient enough to beat out having several mothers and just cloning normally, since you can fit more then 1 mother under a small veg light easily anyway.
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Old 09-14-2009, 11:15 PM
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Originally Posted by Darkuser View Post
Is the cytokinin and auxins in the compound this guy has itself or do you get it elsewhere?

It sounds to me like it basically turns a small piece of 0.3-1cm length plant material into a seed. You will be able to get all your clones from a single mother, but instead of having a rooted 4 inch plant in a week, you have a rooted "seed".

As for most plants, an entire regenerated plant with developed roots will be got within 4-11 days. After 15-50 days training, it can be moved out of the garden for transplanting. Rapid propagation is factually realized.

Not sure what that '15-50 days training' is supposed to mean either.

Can't imagine the number vs time spent is efficient enough to beat out having several mothers and just cloning normally, since you can fit more then 1 mother under a small veg light easily anyway.
the guy doesnt say what he uses....but that would be my assumtion...
u can buy the hormones and mix them urself....
the idea i have is if u can get multiple clones from 1 leaf then u will have more bud sites on ur plant thus increasing yeild
i think the 15-50 days is shoot formation or humidity control....i know its a long time but some people dont have 2 rooms to grow so it would be more convenient
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Old 09-15-2009, 02:22 AM
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i would love to learn how to do this..plz keep updating...i have a few mother of different kinds....but when i move babies in i always have to mess with the light so if i could find away to get around that....it would be sweet...keep me updated..plz..
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Old 09-15-2009, 02:58 AM
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i would love to learn how to do this..plz keep updating...i have a few mother of different kinds....but when i move babies in i always have to mess with the light so if i could find away to get around that....it would be sweet...keep me updated..plz..
definately!!!!!
i really do plan on trying
just gotta get the hormones,but times r rough right now
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Old 09-15-2009, 03:30 AM
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I googled it and can't find any thing about it but the things you stated already.......sounds pretty cool if it was real
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Old 09-15-2009, 03:43 AM
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I googled it and can't find any thing about it but the things you stated already.......sounds pretty cool if it was real
well if u think about it...all tissue culture is,is plant material and hormones with sterelity......my thoughts r if u cut the stem and expose those cells to the cytokinins,then stick the other end in the medium with rooting hormone ..it will produce shoots after or at the same of the roots
im hoping someone (with experience in this) can comment and let me know whats up
google---ternpc
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Old 09-15-2009, 05:01 PM
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It's highly doubtful you can clone from a leaf. It doesn't have the right type of cells and it lacks any nodes. Where would the shoots come from?
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Old 09-15-2009, 09:06 PM
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It's highly doubtful you can clone from a leaf. It doesn't have the right type of cells and it lacks any nodes. Where would the shoots come from?

That's a rational question just as i had.....i read and their is lot of literature of how successful this technique with many species of plant but nothing on how to do it......I would also like to know more if anybody has some knowledge on this
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Old 09-15-2009, 09:15 PM
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It's highly doubtful you can clone from a leaf. It doesn't have the right type of cells and it lacks any nodes. Where would the shoots come from?
if it doesnt have the right cells then how is tissue culture possible?
nodes come when the shoots form from the hormones that its treated with....and the shoots would form where the callus forms,from the slice made....i.e.tissue culture
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