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  #1    
Old 09-06-2009, 02:09 PM
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Default clone of a clone of a clone of a clone.....
I have an advanced cloning question. Is it ok to be taking clones of clones going on for many generations?

Usually, right before going to flower, I'll take some lower branches from a clone to start the next crop. When these clones are ready to flower, I'll take some more and repeat the process again. This means having no mother.

How many generations can this process go on and will the deeper generation clones start to have any problems?

Thanks, YGB
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Old 09-06-2009, 02:20 PM
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Quote:
Originally Posted by YouGrowBoy View Post
I have an advanced cloning question. Is it ok to be taking clones of clones going on for many generations?

Usually, right before going to flower, I'll take some lower branches from a clone to start the next crop. When these clones are ready to flower, I'll take some more and repeat the process again. This means having no mother.

How many generations can this process go on and will the deeper generation clones start to have any problems?

Thanks, YGB

Ive read this question sooo many times... but im still gonna answer it...
Clones will never have problems that the original plant didnt have. its a clone... that means its the same plant, just growing on its own now. same genetics, unchanged forever.
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Old 09-06-2009, 02:30 PM
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more then fine to repeat cloning on clones. the most important thing to remember is to choose your original mother plant wisely.. look for characteristics that u want ..high yeilder short height etc.... but no matter how times u clone a clone it is still the same genetic copy of the original plant
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Old 09-06-2009, 02:30 PM
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I've been cloning the same re-veg clone for goin' on 3 years now and haven't noticed any degradation of the species. I'm probably on generation 25 with very healthy vegged plants.
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Old 09-06-2009, 02:31 PM
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Hmm.. as far as I know plants have the same problems as humans.. but im not sure.. though a plant may have the same genetics, the DNA is always being damaged (failure when copying amino acids into DNA), but whilst still repairing itself it cant maintain the ratio of damage to "heal".. so the the longer the same DNA has been copied (cells) the less chance the plant will have of continuing its abilities such as fighting off mold, insects. etc.. its something that is studied right now on living organism were they try to stress a organism (mostly bacteria) to make it "heal" faster then its being damaged.. however im not sure how or if it applies to plants.. but still.. worth mentioning i think

EDIT: Oh.. and btw., i don't know how long time it takes before a vital change is made!
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Old 09-06-2009, 03:41 PM
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So we have 3 people saying it's not a problem and 1 that says it could be.
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Old 09-06-2009, 03:46 PM
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I'm with the one.. Its is unlikely however I have personally had them become depressed. Lack of vigor or potency or both. It kinda comes down to about every factor u can imagine. I am still doing this and it is working great with my strain but I can tell u sensi star (at least mine) only took 6 gens to depress. So to answer ur question. YES u can clone a clone and its not likely ull see any adverse effects BUT it IS POSSIBLE for things to go south. Something about a dna alteration or mutation or something I'm not botanist just speakin of personal experience
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Old 09-07-2009, 09:04 AM
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Quote:
Originally Posted by whutever View Post
Hmm.. as far as I know plants have the same problems as humans.. but im not sure.. though a plant may have the same genetics, the DNA is always being damaged (failure when copying amino acids into DNA), but whilst still repairing itself it cant maintain the ratio of damage to "heal".. so the the longer the same DNA has been copied (cells) the less chance the plant will have of continuing its abilities such as fighting off mold, insects. etc.. its something that is studied right now on living organism were they try to stress a organism (mostly bacteria) to make it "heal" faster then its being damaged.. however im not sure how or if it applies to plants.. but still.. worth mentioning i think

EDIT: Oh.. and btw., i don't know how long time it takes before a vital change is made!
What you saying makes sense but it appears that plants don't have this problem. Understand however that you are referring to somatic DNA (that responsible for meiosis). Mitosis uses a perfect copy to produce offspring.

What seems to be the case with clones is that the plant regenerates from the original DNA just as in mitosis and produces a new born baby clone just as if it produced a seedling.

I think the technique of using somatic DNA caused this issue but it doesn't seem this applies to plant clones. Also, I don't think they even do that anymore. I could be wrong because it's been a long time since I last looked into it.

Anyway, I know a guy with a strain that is 5 years old and each cutting is like a brand new plant.
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Old 09-08-2009, 02:01 AM
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I've kept clones of clones for years, IMO the main factor to keeping the copies as close as possible to the original is lack of environmental stress to keep the major mutations away. Stressed one mother out pretty badly and never had another well-behaved clone generation after. Other 2-4 year old strains which didn't feel the squeeze are still rock solid.
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Old 09-08-2009, 04:04 PM
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Quote:
Originally Posted by freddiemoney View Post
I've kept clones of clones for years, IMO the main factor to keeping the copies as close as possible to the original is lack of environmental stress to keep the major mutations away. Stressed one mother out pretty badly and never had another well-behaved clone generation after. Other 2-4 year old strains which didn't feel the squeeze are still rock solid.
You may have carried over a disease of some kind. Cloning is about as much stress as you can put on a plant.
 

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