Hey guys,

Ive been reading a bit about the whole Plant tissue culture phenomenon, and i was wondering if this method would work very well with cannabis. There are more expensive kits out there, but this is the only one i could find that has a cut n dry description of how it works, for the people out there who havent heard of it yet.

http://cgi.ebay.com/Plant-Tissue-Cul...lenotsupported

Im just curious if it would be effective for somebody who's looking to take like 500 clones at once, without having to have the 20 mother plants that would have to come with it in normal circumstances. If i read everything correctly, i wouldnt even really need ONE mother. So, any opinions on this would really help me out, because it would make things worlds more efficient and convenient for me if this were a plauseable method to apply to a SOG setup.

Space is going to get cramped in my growroom, and not having to have a mother tent would really make things WAY easier on me. But please give your honest opinions only, i dont wanna scrap the mothers i already have going, nor will i, until i know that this method works, and works well.

Thanks for your feedback,
K1.

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i am considering trying this myself

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a moderate shelf space in a clean room and modest lighting and you could culture 500 plants easily. however you must and i stress must be Anal about cleanliness. anything gets into the jar and you can scrap it
everything must be clean. now if i can just find the proper mix of agar and nutes to make it fly

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bs. clean isn't the word. you must be STERILE! if you don't have a chem lab in your house, don't even think about it.

i love how micro culture comes up around here each month. everyone rediscovering that gravity does in fact work...

you haved to be sterile, yes, but you dont need a chem lab. If you'd check out the newer kits that are around today, you'd see that alot of them are boasting that very same fact dude. N i never said that i just discovered it brother, ive known about it for a while, i just never considered using it until i had the thought that i could replace the need for mother plants, by using this method, which is totally plauseable, it would just take a little longer for my clones to get up off the ground. If i were to work it into my perpetual schedule though, and push everything back by a month, i'd still be able to go an lb/two weeks if i wanted to. I just wanted to know if there were people out there who agreed with me on the matter.

There's a synic everywhere ya go nowadays, lol....

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keep in mind that tissue propagation can/does take more than 2/4 months for usable starts to develop. It is a myth that mothers can be negated. raw genetic material must be obtained freshly batch to batch. alternatively all that is required can be obtained from a few leaves. Honestly from your description of needs.. 500 just does not warrant this process. Also sterility cannot be over emphasized! tooling the process is expensive unless you have sizable autoclave laying around. the vendors try to make the kits look easy... However, it takes approximately 4-6 months to achieve consistent deliverable results.
Good Luck!

Hey King

I currently work in plant tissue culture/propagation/transformation. I am intimately familiar with all the necessary steps and components, and can pretty much answer any questions you'll have on this subject.

The link you provided gives no details about what they're actually selling you. And trust me, 59.95 aint gonna get you SHIT.
Things you would need to do this correctly:
-A pressure cooker to sterilize containers
-A sterilizable space (I use a laminar flow hood) but you could possibly create a space where the ambient airborn contaminants wont get in the agar. The thing is, you'll not know if your area is sterile until you see whether fungus is growing on the agar, and if it is that will ruin the sterility of that plant and it will have to be tossed.
***Something people dont realize: You need to START with sterile plant matter. WHich means you have to sterilize a seed in 1:2 clorox, sterile water, and a few drops of one of a few types of scientific detergent (at least I assume it's 'scientific' because i've never seen "Tween" anywhere but in the lab). You have to grow the seed sterily, and take cuttings from that.

you'll also need:

-Plant hormones (auxins, cytokinins)
-A gelling agent (agar, gelzan, gelrite, there are a few you can use)
-MS vitamins w/micronutrients
(the above things are the absolute base needed to make a nutrient rich growing medium with the necessary components)
-A magnetic stir plate
-Assorted labware (glass beaker, a few ehrlenmeyer flasks, etc. The hormones have to be added AFTER the steam sterilization of the media, after it's cooled to 50 degrees, so that can be tricky at home.
-Tools that can be sterilized
-enough vessels to do all the cloning you have planned.


Also, there is something called "somaclonal variation". which can/does occur to plant material that is kept in tissue culture for too long. http://en.wikipedia.org/wiki/Somaclonal_variation so keeping a mother in tissue culture too long could start changing your product. (and probably for the worse)

I dont really get what your plan is, you cant keep a mother in those vessels too long (because of what I already mentioned) but also because it is going to keep growing, and VERY quickly outgrow any sort of small vessel you're using, so you'd have to be subculturing (taking some leaf material, putting it in a new jar and growing roots and shoots from it) which would lead to a lot more somaclonal variation. Also cuttings would take a while to form the shoots and roots you want. You'd have to put it in one media formulation to produce shoots, then move it to another one to induce roots. It is probably much more complicated than you might have thought.

anyways, I gotta run. Hopefully i've helped educate without damping your spirit too much. This CAN be done, but it's more complicated than people tend to think.

that is a starter kit. after 30 days you will use the super kit and its around $253.00 for that.
http://www.planttc.com/

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I do PTC in school and at home. Go to http://www.kitchenculturekit.com/ and you can use the free how to using household stuff. Also has links you can go to and buy the couple hormones and plant preservatives you will need, they are super cheap. I get mine from school.
How about 100's of exact replicas in 2 months. ready to plant into what ever medium you want. Start searching craigslist for baby food jars, your going to need hundreds. PTC is very addictive! Think of it like this; you are forcing every cell that makes up that little piece of pot leaf, to grow into a plant. Thousands of cells make up a little 1/4 x 1/2 inch piece of leaf, so find jars as fast as you can and get ready!

p.s. Everyone's a critic and yes you will have a contaminated one or two but for the most part if your slightly competent and can read and follow direction you will have great success. Before I did Plant Tissue culture, I was told the same thing about home mycology and right now I am still growing mushrooms in a home "sterile" environment using Rubbermaid bins as terrariums and one placed on its side as my clean room while I work. Its the same bin I use when I do PTC and I rarely have problems.
http://www.kitchenculturekit.com/
Its what I used in my introductory Horticulture 101 class and had no problems.
Just enjoy what your doing and stay positive.