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Old 10-23-2008, 11:03 AM
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After seeing what you guys think about LEDs, I don't know if anyone would be interested in this, but here goes...

I've started doing tissue cultures for micro propagation and I had some really good results. So I did some research in the advances in DNA manipulation and was surprised at how far things have come.

See the attached pics. One is the manufacture 'cheat sheet' for preparing DNA samples and the other is the directions for my vaporizer. The vaporizer has 3 more steps than the DNA prep! (okay, it is harder than smoking a vaporizer, but not too much)

I've used this (http://www.jbc.org/cgi/content/full/279/38/39767) as a guide, but they only isolated the DNA to make THCA and had to 'feed' it CBNs to get it to make THCA... I'm hoping for the whole package...

Anybody else doing anything like this?

-Loki
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Last edited by Loki7; 10-23-2008 at 05:06 PM..
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Old 10-23-2008, 04:33 PM
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hm.

well, that miniprep procedure is just one tiny little part in a much larger procedure, depending on what it is you're trying to do with the DNA. you'll also need a PCR machine, gel electrophoresis, correct primers, a whole mess of stuff really that i'm not going to list here. I really dont get what you are attempting to do.

also that link doesnt work.
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Old 10-23-2008, 04:35 PM
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here is the link he was trying to use: The Gene Controlling Marijuana Psychoactivity: MOLECULAR CLONING AND HETEROLOGOUS EXPRESSION OF {Delta}1-TETRAHYDROCANNABINOLIC ACID SYNTHASE FROM CANNABIS SATIVA L. -- Sirikantaramas et al. 279 (3: 39767 -- Journal of Biological Chemistry
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Old 10-23-2008, 04:42 PM
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ok, made the link work. hm, interesting paper, i'm going to give it a read.

It wont be long until someone genetically modifies herb, it would probably be as simply as stably upregulating that gene to boost THC production. Stack that with some yeild enhancing genes and you could make some insane plants.
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Old 10-23-2008, 04:45 PM
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What did I do wrong with the link? I used the link button and pasted in the URL... What else do I need to do?

Edit: nvm.. double http://s... oops

-Loki
Last edited by Loki7; 10-23-2008 at 04:56 PM..
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Old 10-23-2008, 04:54 PM
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hm.

well, that miniprep procedure is just one tiny little part in a much larger procedure, depending on what it is you're trying to do with the DNA. you'll also need a PCR machine, gel electrophoresis, correct primers, a whole mess of stuff really that i'm not going to list here. I really dont get what you are attempting to do.

also that link doesnt work.
I know its only one step. I was just using it as an example of how far things have come. And you don't need a PCR any more (well, I don't.. not ~need~).

I've got the restrictive enzymes and pre-cultured transfer bacterium. And I picked up one of these...

and 2 sets of gel casting trays. Along with a centifruge, magnetic stirs, tons of petri dishes, pipettes and test tubes... Custom built a clean box/incubation chamber with cold storage... I'll post some pics of it. The only pic I have right now is it in the pre fab stage...



This is what I was using for tissue cultures... Its was too small to do too much more in, hence the new model...




-Loki
Last edited by Loki7; 10-23-2008 at 05:16 PM..
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Old 10-23-2008, 05:00 PM
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ok, made the link work. hm, interesting paper, i'm going to give it a read.

It wont be long until someone genetically modifies herb, it would probably be as simply as stably upregulating that gene to boost THC production. Stack that with some yeild enhancing genes and you could make some insane plants.
I'm actually hoping to transfer pot DNA into cells from another type of plant and have that plant grow something smokable with THC in it that doesn't look like a pot plant.

I want to grow outdoors. I'd have over 60 acres to use, if it didn't look like pot.

-Loki
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Old 10-23-2008, 06:02 PM
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That would be awesome! Good luck. That would be a nice day job in a decked out lab.
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Old 10-23-2008, 06:17 PM
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That would be awesome! Good luck. That would be a nice day job in a decked out lab.
Sad part about that is the guy that gets that day job, probably won't smoke... Life and the cruel games she plays...

-Loki
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Old 10-24-2008, 05:04 PM
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Sad part about that is the guy that gets that day job, probably won't smoke...

-Loki
hehe, don't be so sure about that...
 
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